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The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43

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The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43. / Keihani, S; Kluever, V; Mandad, S; Bansal, V; Rahman, R; Fritsch, E; Gomes, L Caldi; Gärtner, A; Kügler, S; Urlaub, H; Wren, J D; Bonn, S; Rizzoli, S O; Fornasiero, E F.

in: SCI ADV, Jahrgang 5, Nr. 12, 18.12.2019, S. eaay2670.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Keihani, S, Kluever, V, Mandad, S, Bansal, V, Rahman, R, Fritsch, E, Gomes, LC, Gärtner, A, Kügler, S, Urlaub, H, Wren, JD, Bonn, S, Rizzoli, SO & Fornasiero, EF 2019, 'The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43', SCI ADV, Jg. 5, Nr. 12, S. eaay2670. https://doi.org/10.1126/sciadv.aay2670

APA

Keihani, S., Kluever, V., Mandad, S., Bansal, V., Rahman, R., Fritsch, E., Gomes, L. C., Gärtner, A., Kügler, S., Urlaub, H., Wren, J. D., Bonn, S., Rizzoli, S. O., & Fornasiero, E. F. (2019). The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43. SCI ADV, 5(12), eaay2670. https://doi.org/10.1126/sciadv.aay2670

Vancouver

Keihani S, Kluever V, Mandad S, Bansal V, Rahman R, Fritsch E et al. The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43. SCI ADV. 2019 Dez 18;5(12):eaay2670. https://doi.org/10.1126/sciadv.aay2670

Bibtex

@article{7739a816226441f1b6e0ae65f374e47d,
title = "The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43",
abstract = "The cellular and the molecular mechanisms by which long noncoding RNAs (lncRNAs) may regulate presynaptic function and neuronal activity are largely unexplored. Here, we established an integrated screening strategy to discover lncRNAs implicated in neurotransmitter and synaptic vesicle release. With this approach, we identified neuroLNC, a neuron-specific nuclear lncRNA conserved from rodents to humans. NeuroLNC is tuned by synaptic activity and influences several other essential aspects of neuronal development including calcium influx, neuritogenesis, and neuronal migration in vivo. We defined the molecular interactors of neuroLNC in detail using chromatin isolation by RNA purification, RNA interactome analysis, and protein mass spectrometry. We found that the effects of neuroLNC on synaptic vesicle release require interaction with the RNA-binding protein TDP-43 (TAR DNA binding protein-43) and the selective stabilization of mRNAs encoding for presynaptic proteins. These results provide the first proof of an lncRNA that orchestrates neuronal excitability by influencing presynaptic function.",
keywords = "Animals, Cell Movement/genetics, DNA-Binding Proteins/genetics, HEK293 Cells, Hippocampus/cytology, Humans, Mice, Mice, Transgenic, Neurogenesis/genetics, Neurons/metabolism, Neurotransmitter Agents/metabolism, RNA, Long Noncoding/metabolism, RNA, Messenger/metabolism, Rats, Rats, Wistar, Synaptic Vesicles/metabolism, Transfection",
author = "S Keihani and V Kluever and S Mandad and V Bansal and R Rahman and E Fritsch and Gomes, {L Caldi} and A G{\"a}rtner and S K{\"u}gler and H Urlaub and Wren, {J D} and S Bonn and Rizzoli, {S O} and Fornasiero, {E F}",
note = "Copyright {\textcopyright} 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",
year = "2019",
month = dec,
day = "18",
doi = "10.1126/sciadv.aay2670",
language = "English",
volume = "5",
pages = "eaay2670",
journal = "SCI ADV",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "12",

}

RIS

TY - JOUR

T1 - The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43

AU - Keihani, S

AU - Kluever, V

AU - Mandad, S

AU - Bansal, V

AU - Rahman, R

AU - Fritsch, E

AU - Gomes, L Caldi

AU - Gärtner, A

AU - Kügler, S

AU - Urlaub, H

AU - Wren, J D

AU - Bonn, S

AU - Rizzoli, S O

AU - Fornasiero, E F

N1 - Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

PY - 2019/12/18

Y1 - 2019/12/18

N2 - The cellular and the molecular mechanisms by which long noncoding RNAs (lncRNAs) may regulate presynaptic function and neuronal activity are largely unexplored. Here, we established an integrated screening strategy to discover lncRNAs implicated in neurotransmitter and synaptic vesicle release. With this approach, we identified neuroLNC, a neuron-specific nuclear lncRNA conserved from rodents to humans. NeuroLNC is tuned by synaptic activity and influences several other essential aspects of neuronal development including calcium influx, neuritogenesis, and neuronal migration in vivo. We defined the molecular interactors of neuroLNC in detail using chromatin isolation by RNA purification, RNA interactome analysis, and protein mass spectrometry. We found that the effects of neuroLNC on synaptic vesicle release require interaction with the RNA-binding protein TDP-43 (TAR DNA binding protein-43) and the selective stabilization of mRNAs encoding for presynaptic proteins. These results provide the first proof of an lncRNA that orchestrates neuronal excitability by influencing presynaptic function.

AB - The cellular and the molecular mechanisms by which long noncoding RNAs (lncRNAs) may regulate presynaptic function and neuronal activity are largely unexplored. Here, we established an integrated screening strategy to discover lncRNAs implicated in neurotransmitter and synaptic vesicle release. With this approach, we identified neuroLNC, a neuron-specific nuclear lncRNA conserved from rodents to humans. NeuroLNC is tuned by synaptic activity and influences several other essential aspects of neuronal development including calcium influx, neuritogenesis, and neuronal migration in vivo. We defined the molecular interactors of neuroLNC in detail using chromatin isolation by RNA purification, RNA interactome analysis, and protein mass spectrometry. We found that the effects of neuroLNC on synaptic vesicle release require interaction with the RNA-binding protein TDP-43 (TAR DNA binding protein-43) and the selective stabilization of mRNAs encoding for presynaptic proteins. These results provide the first proof of an lncRNA that orchestrates neuronal excitability by influencing presynaptic function.

KW - Animals

KW - Cell Movement/genetics

KW - DNA-Binding Proteins/genetics

KW - HEK293 Cells

KW - Hippocampus/cytology

KW - Humans

KW - Mice

KW - Mice, Transgenic

KW - Neurogenesis/genetics

KW - Neurons/metabolism

KW - Neurotransmitter Agents/metabolism

KW - RNA, Long Noncoding/metabolism

KW - RNA, Messenger/metabolism

KW - Rats

KW - Rats, Wistar

KW - Synaptic Vesicles/metabolism

KW - Transfection

U2 - 10.1126/sciadv.aay2670

DO - 10.1126/sciadv.aay2670

M3 - SCORING: Journal article

C2 - 31897430

VL - 5

SP - eaay2670

JO - SCI ADV

JF - SCI ADV

SN - 2375-2548

IS - 12

ER -