The impact of non-toxic blue light (453 nm) on cellular antioxidative capacity, TGF-β1 signaling, and myofibrogenesis of human skin fibroblasts
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The impact of non-toxic blue light (453 nm) on cellular antioxidative capacity, TGF-β1 signaling, and myofibrogenesis of human skin fibroblasts. / Krassovka, Julia M; Suschek, Christoph V; Prost, Max; Grotheer, Vera; Schiefer, Jennifer L; Demir, Erhan; Fuchs, Paul C; Windolf, Joachim; Stürmer, Ewa K; Opländer, Christian.
in: J PHOTOCH PHOTOBIO B, Jahrgang 209, 111952, 08.2020.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - The impact of non-toxic blue light (453 nm) on cellular antioxidative capacity, TGF-β1 signaling, and myofibrogenesis of human skin fibroblasts
AU - Krassovka, Julia M
AU - Suschek, Christoph V
AU - Prost, Max
AU - Grotheer, Vera
AU - Schiefer, Jennifer L
AU - Demir, Erhan
AU - Fuchs, Paul C
AU - Windolf, Joachim
AU - Stürmer, Ewa K
AU - Opländer, Christian
N1 - Copyright © 2020 Elsevier B.V. All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - Studies have demonstrated that blue light induces biological effects, such as cell death, and inhibition of proliferation and differentiation. Since blue light at longer wavelength (>440 nm) exerts less injurious effects on cells than at shorter wavelengths, (400-440 nm), we have investigated the impact of non-toxic (LED) blue light at 453 nm wavelength on human skin fibroblasts (hsFBs). We found that besides its decreasing effects on the proliferation rate, repeated blue light irradiations (80 J/cm2) also significantly reduced TGF-β1-induced myofibrogenesis as shown by diminished α-SMA and EDA-FN expression accompanied by reduced protein expression and phosphorylation of ERK 1/2, SMAD 2/3, and p38-key players of TGF-β1-induced myofibrogenesis. In parallel, catalase protein expression, intracellular FAD concentrations as well as NADP+/NADPH ratio were reduced, whereas intracellular reactive oxygen species (ROS) were increased. We postulate that as a molecular mechanism downregulation of catalase and photoreduction of FAD induce intracellular oxidative stress which, in turn, affects the signaling factors of myofibrogenesis leading to a lower rate of α-SMA and EDA-FN expression and, therefore, myofibroblast formation. In conclusion, blue light even at longer wavelengths shows antifibrotic activity and may represent a suitable and safe approach in the treatment of fibrotic skin diseases including hypertrophic scarring and scleroderma.
AB - Studies have demonstrated that blue light induces biological effects, such as cell death, and inhibition of proliferation and differentiation. Since blue light at longer wavelength (>440 nm) exerts less injurious effects on cells than at shorter wavelengths, (400-440 nm), we have investigated the impact of non-toxic (LED) blue light at 453 nm wavelength on human skin fibroblasts (hsFBs). We found that besides its decreasing effects on the proliferation rate, repeated blue light irradiations (80 J/cm2) also significantly reduced TGF-β1-induced myofibrogenesis as shown by diminished α-SMA and EDA-FN expression accompanied by reduced protein expression and phosphorylation of ERK 1/2, SMAD 2/3, and p38-key players of TGF-β1-induced myofibrogenesis. In parallel, catalase protein expression, intracellular FAD concentrations as well as NADP+/NADPH ratio were reduced, whereas intracellular reactive oxygen species (ROS) were increased. We postulate that as a molecular mechanism downregulation of catalase and photoreduction of FAD induce intracellular oxidative stress which, in turn, affects the signaling factors of myofibrogenesis leading to a lower rate of α-SMA and EDA-FN expression and, therefore, myofibroblast formation. In conclusion, blue light even at longer wavelengths shows antifibrotic activity and may represent a suitable and safe approach in the treatment of fibrotic skin diseases including hypertrophic scarring and scleroderma.
KW - Antioxidants/metabolism
KW - Cell Proliferation/radiation effects
KW - Humans
KW - Light
KW - Myofibroblasts/cytology
KW - Oxidative Stress
KW - Signal Transduction/radiation effects
KW - Transforming Growth Factor beta1/metabolism
U2 - 10.1016/j.jphotobiol.2020.111952
DO - 10.1016/j.jphotobiol.2020.111952
M3 - SCORING: Journal article
C2 - 32659647
VL - 209
JO - J PHOTOCH PHOTOBIO B
JF - J PHOTOCH PHOTOBIO B
SN - 1011-1344
M1 - 111952
ER -