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The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.

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The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells. / Karpiuk, Oleksandra; Najafova, Zeynab; Kramer, Frank; Hennion, Magali; Galonska, Christina; König, Annekatrin; Snaidero, Nicolas; Vogel, Tanja; Shchebet, Andrei; Begus-Nahrmann, Yvonne; Kassem, Moustapha; Simons, Mikael; Shcherbata, Halyna; Beissbarth, Tim; Johnsen, Steven A.

in: MOL CELL, Jahrgang 46, Nr. 5, 5, 2012, S. 705-713.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Karpiuk, O, Najafova, Z, Kramer, F, Hennion, M, Galonska, C, König, A, Snaidero, N, Vogel, T, Shchebet, A, Begus-Nahrmann, Y, Kassem, M, Simons, M, Shcherbata, H, Beissbarth, T & Johnsen, SA 2012, 'The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.', MOL CELL, Jg. 46, Nr. 5, 5, S. 705-713. <http://www.ncbi.nlm.nih.gov/pubmed/22681891?dopt=Citation>

APA

Karpiuk, O., Najafova, Z., Kramer, F., Hennion, M., Galonska, C., König, A., Snaidero, N., Vogel, T., Shchebet, A., Begus-Nahrmann, Y., Kassem, M., Simons, M., Shcherbata, H., Beissbarth, T., & Johnsen, S. A. (2012). The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells. MOL CELL, 46(5), 705-713. [5]. http://www.ncbi.nlm.nih.gov/pubmed/22681891?dopt=Citation

Vancouver

Karpiuk O, Najafova Z, Kramer F, Hennion M, Galonska C, König A et al. The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells. MOL CELL. 2012;46(5):705-713. 5.

Bibtex

@article{ff21eea773bf45b09d3021dd64dd5dfc,
title = "The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.",
abstract = "Extensive changes in posttranslational histone modifications accompany the rewiring of the transcriptional program during stem cell differentiation. However, the mechanisms controlling the changes in specific chromatin modifications and their function during differentiation remain only poorly understood. We show that histone H2B monoubiquitination (H2Bub1) significantly increases during differentiation of human mesenchymal stem cells (hMSCs) and various lineage-committed precursor cells and in diverse organisms. Furthermore, the H2B ubiquitin ligase RNF40 is required for the induction of differentiation markers and transcriptional reprogramming of hMSCs. This function is dependent upon CDK9 and the WAC adaptor protein, which are required for H2B monoubiquitination. Finally, we show that RNF40 is required for the resolution of the H3K4me3/H3K27me3 bivalent poised state on lineage-specific genes during the transition from an inactive to an active chromatin conformation. Thus, these data indicate that H2Bub1 is required for maintaining multipotency of hMSCs and plays a central role in controlling stem cell differentiation.",
keywords = "Humans, Protein Processing, Post-Translational, Cell Line, Ubiquitination, Histones/*metabolism, Mesenchymal Stromal Cells/*cytology/metabolism, Adaptor Proteins, Signal Transducing/genetics/physiology, Cell Differentiation/*genetics, Chromatin Assembly and Disassembly, Cyclin-Dependent Kinase 9/genetics/physiology, Multipotent Stem Cells/*cytology/metabolism, Ubiquitin-Protein Ligases/genetics/metabolism/physiology, Humans, Protein Processing, Post-Translational, Cell Line, Ubiquitination, Histones/*metabolism, Mesenchymal Stromal Cells/*cytology/metabolism, Adaptor Proteins, Signal Transducing/genetics/physiology, Cell Differentiation/*genetics, Chromatin Assembly and Disassembly, Cyclin-Dependent Kinase 9/genetics/physiology, Multipotent Stem Cells/*cytology/metabolism, Ubiquitin-Protein Ligases/genetics/metabolism/physiology",
author = "Oleksandra Karpiuk and Zeynab Najafova and Frank Kramer and Magali Hennion and Christina Galonska and Annekatrin K{\"o}nig and Nicolas Snaidero and Tanja Vogel and Andrei Shchebet and Yvonne Begus-Nahrmann and Moustapha Kassem and Mikael Simons and Halyna Shcherbata and Tim Beissbarth and Johnsen, {Steven A.}",
year = "2012",
language = "English",
volume = "46",
pages = "705--713",
journal = "MOL CELL",
issn = "1097-2765",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.

AU - Karpiuk, Oleksandra

AU - Najafova, Zeynab

AU - Kramer, Frank

AU - Hennion, Magali

AU - Galonska, Christina

AU - König, Annekatrin

AU - Snaidero, Nicolas

AU - Vogel, Tanja

AU - Shchebet, Andrei

AU - Begus-Nahrmann, Yvonne

AU - Kassem, Moustapha

AU - Simons, Mikael

AU - Shcherbata, Halyna

AU - Beissbarth, Tim

AU - Johnsen, Steven A.

PY - 2012

Y1 - 2012

N2 - Extensive changes in posttranslational histone modifications accompany the rewiring of the transcriptional program during stem cell differentiation. However, the mechanisms controlling the changes in specific chromatin modifications and their function during differentiation remain only poorly understood. We show that histone H2B monoubiquitination (H2Bub1) significantly increases during differentiation of human mesenchymal stem cells (hMSCs) and various lineage-committed precursor cells and in diverse organisms. Furthermore, the H2B ubiquitin ligase RNF40 is required for the induction of differentiation markers and transcriptional reprogramming of hMSCs. This function is dependent upon CDK9 and the WAC adaptor protein, which are required for H2B monoubiquitination. Finally, we show that RNF40 is required for the resolution of the H3K4me3/H3K27me3 bivalent poised state on lineage-specific genes during the transition from an inactive to an active chromatin conformation. Thus, these data indicate that H2Bub1 is required for maintaining multipotency of hMSCs and plays a central role in controlling stem cell differentiation.

AB - Extensive changes in posttranslational histone modifications accompany the rewiring of the transcriptional program during stem cell differentiation. However, the mechanisms controlling the changes in specific chromatin modifications and their function during differentiation remain only poorly understood. We show that histone H2B monoubiquitination (H2Bub1) significantly increases during differentiation of human mesenchymal stem cells (hMSCs) and various lineage-committed precursor cells and in diverse organisms. Furthermore, the H2B ubiquitin ligase RNF40 is required for the induction of differentiation markers and transcriptional reprogramming of hMSCs. This function is dependent upon CDK9 and the WAC adaptor protein, which are required for H2B monoubiquitination. Finally, we show that RNF40 is required for the resolution of the H3K4me3/H3K27me3 bivalent poised state on lineage-specific genes during the transition from an inactive to an active chromatin conformation. Thus, these data indicate that H2Bub1 is required for maintaining multipotency of hMSCs and plays a central role in controlling stem cell differentiation.

KW - Humans

KW - Protein Processing, Post-Translational

KW - Cell Line

KW - Ubiquitination

KW - Histones/metabolism

KW - Mesenchymal Stromal Cells/cytology/metabolism

KW - Adaptor Proteins, Signal Transducing/genetics/physiology

KW - Cell Differentiation/genetics

KW - Chromatin Assembly and Disassembly

KW - Cyclin-Dependent Kinase 9/genetics/physiology

KW - Multipotent Stem Cells/cytology/metabolism

KW - Ubiquitin-Protein Ligases/genetics/metabolism/physiology

KW - Humans

KW - Protein Processing, Post-Translational

KW - Cell Line

KW - Ubiquitination

KW - Histones/metabolism

KW - Mesenchymal Stromal Cells/cytology/metabolism

KW - Adaptor Proteins, Signal Transducing/genetics/physiology

KW - Cell Differentiation/genetics

KW - Chromatin Assembly and Disassembly

KW - Cyclin-Dependent Kinase 9/genetics/physiology

KW - Multipotent Stem Cells/cytology/metabolism

KW - Ubiquitin-Protein Ligases/genetics/metabolism/physiology

M3 - SCORING: Journal article

VL - 46

SP - 705

EP - 713

JO - MOL CELL

JF - MOL CELL

SN - 1097-2765

IS - 5

M1 - 5

ER -