The hemicochlea preparation of the guinea pig and other mammalian cochleae.

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The hemicochlea preparation of the guinea pig and other mammalian cochleae. / Teudt, Ingo; Richter, Claus-Peter.

in: J NEUROSCI METH, Jahrgang 162, Nr. 1-2, 1-2, 2007, S. 187-197.

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@article{b6a6531db789448cb1391738a664d1a5,
title = "The hemicochlea preparation of the guinea pig and other mammalian cochleae.",
abstract = "The hemicochlea and its slice preparation is a novel method that allows access to various cochlear structures without the physical distortion that typically occurs from tissue dehydration during the embedding process. Therefore, the hemicochlea preparation provides an excellent model to study (1) cochlear morphology during cochlear development, (2) malformation caused by genetic defects, (3) changes related to diseases, (4) sensory physiology, (5) cochlear micromechanics, and (6) the expression of proteins by immunohistochemistry. This paper describes in detail the method of slicing hemicochleae for different mammalian species, including mice, rats, gerbils, guinea pigs, pigs, and human temporal bones. Furthermore, guinea pig cochleae are used as an example to provide cochlear dimensions of important anatomical structures. The values obtained in eight guinea pig hemicochleae are compared to published values, and upon review, discrepancies do exist. For example, gelatinous structures, such as the tectorial membrane, appear larger in the hemicochlea when compared to traditional embedding. Dimensions obtained for selected cochlear structures at different locations along the guinea pig cochleae provide an improved basis for cochlear models.",
author = "Ingo Teudt and Claus-Peter Richter",
year = "2007",
language = "Deutsch",
volume = "162",
pages = "187--197",
journal = "J NEUROSCI METH",
issn = "0165-0270",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - The hemicochlea preparation of the guinea pig and other mammalian cochleae.

AU - Teudt, Ingo

AU - Richter, Claus-Peter

PY - 2007

Y1 - 2007

N2 - The hemicochlea and its slice preparation is a novel method that allows access to various cochlear structures without the physical distortion that typically occurs from tissue dehydration during the embedding process. Therefore, the hemicochlea preparation provides an excellent model to study (1) cochlear morphology during cochlear development, (2) malformation caused by genetic defects, (3) changes related to diseases, (4) sensory physiology, (5) cochlear micromechanics, and (6) the expression of proteins by immunohistochemistry. This paper describes in detail the method of slicing hemicochleae for different mammalian species, including mice, rats, gerbils, guinea pigs, pigs, and human temporal bones. Furthermore, guinea pig cochleae are used as an example to provide cochlear dimensions of important anatomical structures. The values obtained in eight guinea pig hemicochleae are compared to published values, and upon review, discrepancies do exist. For example, gelatinous structures, such as the tectorial membrane, appear larger in the hemicochlea when compared to traditional embedding. Dimensions obtained for selected cochlear structures at different locations along the guinea pig cochleae provide an improved basis for cochlear models.

AB - The hemicochlea and its slice preparation is a novel method that allows access to various cochlear structures without the physical distortion that typically occurs from tissue dehydration during the embedding process. Therefore, the hemicochlea preparation provides an excellent model to study (1) cochlear morphology during cochlear development, (2) malformation caused by genetic defects, (3) changes related to diseases, (4) sensory physiology, (5) cochlear micromechanics, and (6) the expression of proteins by immunohistochemistry. This paper describes in detail the method of slicing hemicochleae for different mammalian species, including mice, rats, gerbils, guinea pigs, pigs, and human temporal bones. Furthermore, guinea pig cochleae are used as an example to provide cochlear dimensions of important anatomical structures. The values obtained in eight guinea pig hemicochleae are compared to published values, and upon review, discrepancies do exist. For example, gelatinous structures, such as the tectorial membrane, appear larger in the hemicochlea when compared to traditional embedding. Dimensions obtained for selected cochlear structures at different locations along the guinea pig cochleae provide an improved basis for cochlear models.

M3 - SCORING: Zeitschriftenaufsatz

VL - 162

SP - 187

EP - 197

JO - J NEUROSCI METH

JF - J NEUROSCI METH

SN - 0165-0270

IS - 1-2

M1 - 1-2

ER -