The germ cell nuclear factor mGCNF is expressed in the developing nervous system
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The germ cell nuclear factor mGCNF is expressed in the developing nervous system. / Süsens, U; Aguiluz, J B; Evans, R M; Borgmeyer, U.
in: DEV NEUROSCI-BASEL, Jahrgang 19, Nr. 5, 1997, S. 410-20.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The germ cell nuclear factor mGCNF is expressed in the developing nervous system
AU - Süsens, U
AU - Aguiluz, J B
AU - Evans, R M
AU - Borgmeyer, U
PY - 1997
Y1 - 1997
N2 - Using a reinoic acid receptor hybridization probe, we have isolated a mouse embryonic cDNA that encodes the germ cell nuclear factor (mGCNF). The in vitro translated protein binds specifically to the direct repeat of the sequence-AGGTCA-which is characteristic of a subclass of nuclear receptors, albeit with a spacing of zero that is unique among the receptors. Northern analysis shows embryonic expression after mouse gastrulation and during early organogenesis, as well as expression in the adult testis. The message level decreases during embryonic development. Whereas there are two transcripts in the testis, only the larger one is found in embryos. In situ analysis of embryos at days 6.5-10.5 of gestation shows that, in the early postimplantation period, high transcript levels are found in ectodermal cells and in the primitive streak. During further development the expression becomes more restricted. Mainly cells of the developing nervous system are expressing the receptor. Our findings imply that GCNF is involved in two apparently disparate developmental events.
AB - Using a reinoic acid receptor hybridization probe, we have isolated a mouse embryonic cDNA that encodes the germ cell nuclear factor (mGCNF). The in vitro translated protein binds specifically to the direct repeat of the sequence-AGGTCA-which is characteristic of a subclass of nuclear receptors, albeit with a spacing of zero that is unique among the receptors. Northern analysis shows embryonic expression after mouse gastrulation and during early organogenesis, as well as expression in the adult testis. The message level decreases during embryonic development. Whereas there are two transcripts in the testis, only the larger one is found in embryos. In situ analysis of embryos at days 6.5-10.5 of gestation shows that, in the early postimplantation period, high transcript levels are found in ectodermal cells and in the primitive streak. During further development the expression becomes more restricted. Mainly cells of the developing nervous system are expressing the receptor. Our findings imply that GCNF is involved in two apparently disparate developmental events.
KW - Amino Acid Sequence
KW - Animals
KW - Binding, Competitive
KW - Blotting, Northern
KW - COS Cells
KW - DNA Probes
KW - DNA, Complementary
KW - DNA-Binding Proteins
KW - Ectoderm
KW - Embryonic and Fetal Development
KW - Gastrula
KW - Gene Expression Regulation, Developmental
KW - Gene Library
KW - In Situ Hybridization
KW - Male
KW - Mice
KW - Nervous System
KW - Nuclear Receptor Subfamily 6, Group A, Member 1
KW - Nucleic Acid Hybridization
KW - Oocytes
KW - Protein Structure, Tertiary
KW - RNA, Messenger
KW - Receptors, Cytoplasmic and Nuclear
KW - Receptors, Retinoic Acid
KW - Recombinant Fusion Proteins
KW - Spermatocytes
KW - Time Factors
M3 - SCORING: Journal article
C2 - 9323461
VL - 19
SP - 410
EP - 420
JO - DEV NEUROSCI-BASEL
JF - DEV NEUROSCI-BASEL
SN - 0378-5866
IS - 5
ER -
