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The European antibody network's practical guide to finding and validating suitable antibodies for research

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The European antibody network's practical guide to finding and validating suitable antibodies for research. / Roncador, Giovanna; Engel, Pablo; Maestre, Lorena; Anderson, Amanda P; Cordell, Jacqueline L; Cragg, Mark S; Šerbec, Vladka Č; Jones, Margaret; Lisnic, Vanda J; Kremer, Leonor; Li, Demin; Nolte, Friedrich; Pascual, Núria; Rodríguez-Barbosa, Jose-Ignacio; Torensma, Ruurd; Turley, Helen; Pulford, Karen; Banham, Alison H.

in: MABS-AUSTIN, Jahrgang 8, Nr. 1, 02.01.2016, S. 27-36.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Roncador, G, Engel, P, Maestre, L, Anderson, AP, Cordell, JL, Cragg, MS, Šerbec, VČ, Jones, M, Lisnic, VJ, Kremer, L, Li, D, Nolte, F, Pascual, N, Rodríguez-Barbosa, J-I, Torensma, R, Turley, H, Pulford, K & Banham, AH 2016, 'The European antibody network's practical guide to finding and validating suitable antibodies for research', MABS-AUSTIN, Jg. 8, Nr. 1, S. 27-36. https://doi.org/10.1080/19420862.2015.1100787

APA

Roncador, G., Engel, P., Maestre, L., Anderson, A. P., Cordell, J. L., Cragg, M. S., Šerbec, V. Č., Jones, M., Lisnic, V. J., Kremer, L., Li, D., Nolte, F., Pascual, N., Rodríguez-Barbosa, J-I., Torensma, R., Turley, H., Pulford, K., & Banham, A. H. (2016). The European antibody network's practical guide to finding and validating suitable antibodies for research. MABS-AUSTIN, 8(1), 27-36. https://doi.org/10.1080/19420862.2015.1100787

Vancouver

Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS et al. The European antibody network's practical guide to finding and validating suitable antibodies for research. MABS-AUSTIN. 2016 Jan 2;8(1):27-36. https://doi.org/10.1080/19420862.2015.1100787

Bibtex

@article{f216c5f8158b4ff78373e423f167838f,
title = "The European antibody network's practical guide to finding and validating suitable antibodies for research",
abstract = "Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.",
author = "Giovanna Roncador and Pablo Engel and Lorena Maestre and Anderson, {Amanda P} and Cordell, {Jacqueline L} and Cragg, {Mark S} and {\v S}erbec, {Vladka {\v C}} and Margaret Jones and Lisnic, {Vanda J} and Leonor Kremer and Demin Li and Friedrich Nolte and N{\'u}ria Pascual and Jose-Ignacio Rodr{\'i}guez-Barbosa and Ruurd Torensma and Helen Turley and Karen Pulford and Banham, {Alison H}",
year = "2016",
month = jan,
day = "2",
doi = "10.1080/19420862.2015.1100787",
language = "English",
volume = "8",
pages = "27--36",
journal = "MABS-AUSTIN",
issn = "1942-0862",
publisher = "LANDES BIOSCIENCE",
number = "1",

}

RIS

TY - JOUR

T1 - The European antibody network's practical guide to finding and validating suitable antibodies for research

AU - Roncador, Giovanna

AU - Engel, Pablo

AU - Maestre, Lorena

AU - Anderson, Amanda P

AU - Cordell, Jacqueline L

AU - Cragg, Mark S

AU - Šerbec, Vladka Č

AU - Jones, Margaret

AU - Lisnic, Vanda J

AU - Kremer, Leonor

AU - Li, Demin

AU - Nolte, Friedrich

AU - Pascual, Núria

AU - Rodríguez-Barbosa, Jose-Ignacio

AU - Torensma, Ruurd

AU - Turley, Helen

AU - Pulford, Karen

AU - Banham, Alison H

PY - 2016/1/2

Y1 - 2016/1/2

N2 - Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.

AB - Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.

U2 - 10.1080/19420862.2015.1100787

DO - 10.1080/19420862.2015.1100787

M3 - SCORING: Journal article

C2 - 26418356

VL - 8

SP - 27

EP - 36

JO - MABS-AUSTIN

JF - MABS-AUSTIN

SN - 1942-0862

IS - 1

ER -