The erg-like potassium current in rat lactotrophs

Standard

The erg-like potassium current in rat lactotrophs. / Schäfer, R; Wulfsen, I; Behrens, S; Weinsberg, F; Bauer, Christiane K.; Schwarz, J R.

in: J PHYSIOL-LONDON, Jahrgang 518, Nr. 2, 1999, S. 401-416.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Schäfer, R, Wulfsen, I, Behrens, S, Weinsberg, F, Bauer, CK & Schwarz, JR 1999, 'The erg-like potassium current in rat lactotrophs', J PHYSIOL-LONDON, Jg. 518, Nr. 2, S. 401-416. https://doi.org/10.1111/j.1469-7793.1999.0401p.x

APA

Schäfer, R., Wulfsen, I., Behrens, S., Weinsberg, F., Bauer, C. K., & Schwarz, J. R. (1999). The erg-like potassium current in rat lactotrophs. J PHYSIOL-LONDON, 518(2), 401-416. https://doi.org/10.1111/j.1469-7793.1999.0401p.x

Vancouver

Schäfer R, Wulfsen I, Behrens S, Weinsberg F, Bauer CK, Schwarz JR. The erg-like potassium current in rat lactotrophs. J PHYSIOL-LONDON. 1999;518(2):401-416. https://doi.org/10.1111/j.1469-7793.1999.0401p.x

Bibtex

@article{e323e9109fd34ac29305b8f417bd34f5,
title = "The erg-like potassium current in rat lactotrophs",
abstract = "1. The ether-{\`a}-go-go-related gene (erg)-like K+ current in rat lactotrophs from primary culture was characterized and compared with that in clonal rat pituitary cells (GH3/B6). The class III antiarrhythmic E-4031 known to block specifically erg K+ channels was used to isolate the erg-like current as the E-4031-sensitive current. The experiments were performed in 150 mM K+ external solution using the patch-clamp technique. 2. The erg-like K+ current elicited with hyperpolarizing pulses negative to -100 mV consisted of a fast and a pronounced slowly deactivating current component. The contribution of the slow component to the total current amplitude was potential dependent and varied from cell to cell. At -100 mV it ranged from 50 to 85% and at -140 mV from 21 to 45%. 3. The potential-dependent channel availability curves determined with 2 s prepulses were fitted with the sum of two Boltzmann functions. The function related to the slowly deactivating component of the erg-like current was shifted by more than 40 mV to more negative membrane potentials compared with that of the fast component. 4. In contrast to that of native lactotrophs studied under identical conditions, the erg-like K+ current of GH3/B6 cells was characterized by a predominant fast deactivating current component, with similar kinetic and steady-state properties to the fast deactivating current component of native lactotrophs. 5. Thyrotrophin-releasing hormone reduced the erg-like current in native lactotrophs via an intracellular signal cascade which seemed to involve a pathway independent from protein kinase A and protein kinase C. 6. RT-PCR studies on cytoplasm from single lactotrophs revealed the presence of mRNA of the rat homologue of the human ether-{\`a}-go-go-related gene HERG (r-erg1) as well as mRNA of the two other cloned r-erg cDNAs (r-erg2 and r-erg3) in different combinations. In GH3/B6 cells, only the transcripts of r-erg1 and r-erg2 were found.",
author = "R Sch{\"a}fer and I Wulfsen and S Behrens and F Weinsberg and Bauer, {Christiane K.} and Schwarz, {J R}",
year = "1999",
doi = "10.1111/j.1469-7793.1999.0401p.x",
language = "English",
volume = "518",
pages = "401--416",
journal = "J PHYSIOL-LONDON",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - The erg-like potassium current in rat lactotrophs

AU - Schäfer, R

AU - Wulfsen, I

AU - Behrens, S

AU - Weinsberg, F

AU - Bauer, Christiane K.

AU - Schwarz, J R

PY - 1999

Y1 - 1999

N2 - 1. The ether-à-go-go-related gene (erg)-like K+ current in rat lactotrophs from primary culture was characterized and compared with that in clonal rat pituitary cells (GH3/B6). The class III antiarrhythmic E-4031 known to block specifically erg K+ channels was used to isolate the erg-like current as the E-4031-sensitive current. The experiments were performed in 150 mM K+ external solution using the patch-clamp technique. 2. The erg-like K+ current elicited with hyperpolarizing pulses negative to -100 mV consisted of a fast and a pronounced slowly deactivating current component. The contribution of the slow component to the total current amplitude was potential dependent and varied from cell to cell. At -100 mV it ranged from 50 to 85% and at -140 mV from 21 to 45%. 3. The potential-dependent channel availability curves determined with 2 s prepulses were fitted with the sum of two Boltzmann functions. The function related to the slowly deactivating component of the erg-like current was shifted by more than 40 mV to more negative membrane potentials compared with that of the fast component. 4. In contrast to that of native lactotrophs studied under identical conditions, the erg-like K+ current of GH3/B6 cells was characterized by a predominant fast deactivating current component, with similar kinetic and steady-state properties to the fast deactivating current component of native lactotrophs. 5. Thyrotrophin-releasing hormone reduced the erg-like current in native lactotrophs via an intracellular signal cascade which seemed to involve a pathway independent from protein kinase A and protein kinase C. 6. RT-PCR studies on cytoplasm from single lactotrophs revealed the presence of mRNA of the rat homologue of the human ether-à-go-go-related gene HERG (r-erg1) as well as mRNA of the two other cloned r-erg cDNAs (r-erg2 and r-erg3) in different combinations. In GH3/B6 cells, only the transcripts of r-erg1 and r-erg2 were found.

AB - 1. The ether-à-go-go-related gene (erg)-like K+ current in rat lactotrophs from primary culture was characterized and compared with that in clonal rat pituitary cells (GH3/B6). The class III antiarrhythmic E-4031 known to block specifically erg K+ channels was used to isolate the erg-like current as the E-4031-sensitive current. The experiments were performed in 150 mM K+ external solution using the patch-clamp technique. 2. The erg-like K+ current elicited with hyperpolarizing pulses negative to -100 mV consisted of a fast and a pronounced slowly deactivating current component. The contribution of the slow component to the total current amplitude was potential dependent and varied from cell to cell. At -100 mV it ranged from 50 to 85% and at -140 mV from 21 to 45%. 3. The potential-dependent channel availability curves determined with 2 s prepulses were fitted with the sum of two Boltzmann functions. The function related to the slowly deactivating component of the erg-like current was shifted by more than 40 mV to more negative membrane potentials compared with that of the fast component. 4. In contrast to that of native lactotrophs studied under identical conditions, the erg-like K+ current of GH3/B6 cells was characterized by a predominant fast deactivating current component, with similar kinetic and steady-state properties to the fast deactivating current component of native lactotrophs. 5. Thyrotrophin-releasing hormone reduced the erg-like current in native lactotrophs via an intracellular signal cascade which seemed to involve a pathway independent from protein kinase A and protein kinase C. 6. RT-PCR studies on cytoplasm from single lactotrophs revealed the presence of mRNA of the rat homologue of the human ether-à-go-go-related gene HERG (r-erg1) as well as mRNA of the two other cloned r-erg cDNAs (r-erg2 and r-erg3) in different combinations. In GH3/B6 cells, only the transcripts of r-erg1 and r-erg2 were found.

U2 - 10.1111/j.1469-7793.1999.0401p.x

DO - 10.1111/j.1469-7793.1999.0401p.x

M3 - SCORING: Journal article

VL - 518

SP - 401

EP - 416

JO - J PHYSIOL-LONDON

JF - J PHYSIOL-LONDON

SN - 0022-3751

IS - 2

ER -