The effect of ultra-high molecular weight polyethylene wear debris on MG63 osteosarcoma cells in vitro.
Standard
The effect of ultra-high molecular weight polyethylene wear debris on MG63 osteosarcoma cells in vitro. / Dean, D D; Schwartz, Z; Liu, Y; Blanchard, C R; Agrawal, C M; Mabrey, J D; Sylvia, V L; Lohmann, Christoph; Boyan, B D.
in: J BONE JOINT SURG AM, Jahrgang 81, Nr. 4, 4, 1999, S. 452-461.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - The effect of ultra-high molecular weight polyethylene wear debris on MG63 osteosarcoma cells in vitro.
AU - Dean, D D
AU - Schwartz, Z
AU - Liu, Y
AU - Blanchard, C R
AU - Agrawal, C M
AU - Mabrey, J D
AU - Sylvia, V L
AU - Lohmann, Christoph
AU - Boyan, B D
PY - 1999
Y1 - 1999
N2 - BACKGROUND: Focal osteolysis due to ultra-high molecular weight polyethylene wear debris involves effects on both bone resorption and bone formation. METHODS: The response of MG63 osteoblast-like osteosarcoma cells to ultra-high molecular weight polyethylene wear debris isolated by enzymatic digestion of granulomatous tissue obtained from the sites of failed total hip arthroplasties was examined. Scanning electron microscopy, particle-size analysis, and Fourier transform infrared spectroscopy were used to characterize the number, morphology, size distribution, and chemical composition of the particles. Cell response was assessed by adding particles at varying dilutions to confluent cultures and measuring changes in cell proliferation (number of cells and [3H]-thymidine incorporation), osteoblast function (alkaline-phosphatase-specific activity and osteocalcin production), matrix production (collagen production and proteoglycan sulfation), and local cytokine production (prostaglandin-E2 production). RESULTS: The mean size of the particles was 0.60 micrometer, and 95 percent of the particles had a size of less than 1.5 micrometers. The number of particles per gram of tissue ranged from 1.39 to 3.38x10(9). Three of the four batches of particles were endotoxin-free. Exposure of the cells to particles of wear debris significantly increased the number of cells (p
AB - BACKGROUND: Focal osteolysis due to ultra-high molecular weight polyethylene wear debris involves effects on both bone resorption and bone formation. METHODS: The response of MG63 osteoblast-like osteosarcoma cells to ultra-high molecular weight polyethylene wear debris isolated by enzymatic digestion of granulomatous tissue obtained from the sites of failed total hip arthroplasties was examined. Scanning electron microscopy, particle-size analysis, and Fourier transform infrared spectroscopy were used to characterize the number, morphology, size distribution, and chemical composition of the particles. Cell response was assessed by adding particles at varying dilutions to confluent cultures and measuring changes in cell proliferation (number of cells and [3H]-thymidine incorporation), osteoblast function (alkaline-phosphatase-specific activity and osteocalcin production), matrix production (collagen production and proteoglycan sulfation), and local cytokine production (prostaglandin-E2 production). RESULTS: The mean size of the particles was 0.60 micrometer, and 95 percent of the particles had a size of less than 1.5 micrometers. The number of particles per gram of tissue ranged from 1.39 to 3.38x10(9). Three of the four batches of particles were endotoxin-free. Exposure of the cells to particles of wear debris significantly increased the number of cells (p
M3 - SCORING: Zeitschriftenaufsatz
VL - 81
SP - 452
EP - 461
JO - J BONE JOINT SURG AM
JF - J BONE JOINT SURG AM
SN - 0021-9355
IS - 4
M1 - 4
ER -
