The effect of mesenchymal stem cell osteoblastic differentiation on the mechanical properties of engineered bone-like tissue.
Standard
The effect of mesenchymal stem cell osteoblastic differentiation on the mechanical properties of engineered bone-like tissue. / Naito, Hiroshi; Dohi, Yoshiko; Zimmermann, Wolfram-Hubertus; Tojo, Takashi; Takasawa, Shin; Eschenhagen, Thomas; Taniguchi, Shigeki.
in: TISSUE ENG PT A, Jahrgang 17, Nr. 17-18, 17-18, 2011, S. 2321-2329.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - The effect of mesenchymal stem cell osteoblastic differentiation on the mechanical properties of engineered bone-like tissue.
AU - Naito, Hiroshi
AU - Dohi, Yoshiko
AU - Zimmermann, Wolfram-Hubertus
AU - Tojo, Takashi
AU - Takasawa, Shin
AU - Eschenhagen, Thomas
AU - Taniguchi, Shigeki
PY - 2011
Y1 - 2011
N2 - Mesenchymal stem cells (MSCs) can give rise to osteoblasts and have therefore been suggested as a cell source for bone engineering. Here we hypothesized that MSC osteoblastic differentiation and maturation can be supported by three-dimensional cultures in collagen hydrogels (hydrogel culture) to ultimately give rise to mechanically robust bone-like tissue. We first compared the osteoblastic differentiation efficiency of MSCs using osteoinductive supplements (?-glycerophosphate, vitamin C, and dexamethasone) in a hydrogel culture and in a two-dimensional culture (2D culture) by assessing surrogate parameters for osteoblastic differentiation, including osteocalcin (OC) secretion and calcium (Ca) deposition. We next constructed ring-shaped bone-like tissues using MSCs in the hydrogel cultures, and assessed their mechanical (strain-strain analysis), biochemical/molecular (OC secretion, Ca deposition, and Runx2/osterix mRNA levels), and morphological (von Kossa staining) properties. OC secretions and Ca depositions were significantly higher in the hydrogel cultures than those in the 2D cultures, suggesting better osteoblastic differentiation and maturation in the hydrogel cultures. Collagen hydrogel-based ring-shaped bone-like tissues conditioned with osteoinductive supplements developed enhanced biomechanical properties, including high tissue stiffness and ultimate burst strength, superior molecular/biochemical properties, and morphological signs typically found in mineralized bone. These results may be exploited not only to generate bioartificial bone, but also to elucidate the basic mechanisms of bone physiology.
AB - Mesenchymal stem cells (MSCs) can give rise to osteoblasts and have therefore been suggested as a cell source for bone engineering. Here we hypothesized that MSC osteoblastic differentiation and maturation can be supported by three-dimensional cultures in collagen hydrogels (hydrogel culture) to ultimately give rise to mechanically robust bone-like tissue. We first compared the osteoblastic differentiation efficiency of MSCs using osteoinductive supplements (?-glycerophosphate, vitamin C, and dexamethasone) in a hydrogel culture and in a two-dimensional culture (2D culture) by assessing surrogate parameters for osteoblastic differentiation, including osteocalcin (OC) secretion and calcium (Ca) deposition. We next constructed ring-shaped bone-like tissues using MSCs in the hydrogel cultures, and assessed their mechanical (strain-strain analysis), biochemical/molecular (OC secretion, Ca deposition, and Runx2/osterix mRNA levels), and morphological (von Kossa staining) properties. OC secretions and Ca depositions were significantly higher in the hydrogel cultures than those in the 2D cultures, suggesting better osteoblastic differentiation and maturation in the hydrogel cultures. Collagen hydrogel-based ring-shaped bone-like tissues conditioned with osteoinductive supplements developed enhanced biomechanical properties, including high tissue stiffness and ultimate burst strength, superior molecular/biochemical properties, and morphological signs typically found in mineralized bone. These results may be exploited not only to generate bioartificial bone, but also to elucidate the basic mechanisms of bone physiology.
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Biomechanics
KW - Cell Culture Techniques
KW - Tissue Engineering/methods
KW - Cell Differentiation/physiology
KW - Mesenchymal Stem Cells/cytology
KW - Hydrogel/chemistry
KW - Osteoblasts/cytology
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Biomechanics
KW - Cell Culture Techniques
KW - Tissue Engineering/methods
KW - Cell Differentiation/physiology
KW - Mesenchymal Stem Cells/cytology
KW - Hydrogel/chemistry
KW - Osteoblasts/cytology
M3 - SCORING: Journal article
VL - 17
SP - 2321
EP - 2329
JO - TISSUE ENG PT A
JF - TISSUE ENG PT A
SN - 1937-3341
IS - 17-18
M1 - 17-18
ER -
