The development of the collagen fibre network in tissue-engineered cartilage constructs in vivo. Engineered cartilage reorganises fibre network.
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The development of the collagen fibre network in tissue-engineered cartilage constructs in vivo. Engineered cartilage reorganises fibre network. / Paetzold, H; Goepfert, C; Huber, G; Hoenig, E; Pörtner, R; Schilling, A F; Meenen, Norbert; Morlock, M M.
in: EUR CELLS MATER, Jahrgang 23, 2012, S. 209-221.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The development of the collagen fibre network in tissue-engineered cartilage constructs in vivo. Engineered cartilage reorganises fibre network.
AU - Paetzold, H
AU - Goepfert, C
AU - Huber, G
AU - Hoenig, E
AU - Pörtner, R
AU - Schilling, A F
AU - Meenen, Norbert
AU - Morlock, M M
PY - 2012
Y1 - 2012
N2 - For long term durability of tissue-engineered cartilage implanted in vivo, the development of the collagen fibre network orientation is essential as well as the distribution of collagen, since expanded chondrocytes are known to synthesise collagen type I. Typically, these properties differ strongly between native and tissue-engineered cartilage. Nonetheless, the clinical results of a pilot study with implanted tissue-engineered cartilage in pigs were surprisingly good. The purpose of this study was therefore to analyse if the structure and composition of the artificial cartilage tissue changes in the first 52 weeks after implantation. Thus, collagen network orientation and collagen type distribution in tissue-engineered cartilage-carrier-constructs implanted in the knee joints of Göttinger minipigs for 2, 26 or 52 weeks have been further investigated by processing digitised microscopy images of histological sections. The comparison to native cartilage demonstrated that fibre orientation over the cartilage depth has a clear tendency towards native cartilage with increasing time of implantation. After 2 weeks, the collagen fibres of the superficial zone were oriented parallel to the articular surface with little anisotropy present in the middle and deep zones. Overall, fibre orientation and collagen distribution within the implants were less homogenous than in native cartilage tissue. Despite a relatively low number of specimens, the consistent observation of a continuous approximation to native tissue is very promising and suggests that it may not be necessary to engineer the perfect tissue for implantation but rather to provide an intermediate solution to help the body to heal itself.
AB - For long term durability of tissue-engineered cartilage implanted in vivo, the development of the collagen fibre network orientation is essential as well as the distribution of collagen, since expanded chondrocytes are known to synthesise collagen type I. Typically, these properties differ strongly between native and tissue-engineered cartilage. Nonetheless, the clinical results of a pilot study with implanted tissue-engineered cartilage in pigs were surprisingly good. The purpose of this study was therefore to analyse if the structure and composition of the artificial cartilage tissue changes in the first 52 weeks after implantation. Thus, collagen network orientation and collagen type distribution in tissue-engineered cartilage-carrier-constructs implanted in the knee joints of Göttinger minipigs for 2, 26 or 52 weeks have been further investigated by processing digitised microscopy images of histological sections. The comparison to native cartilage demonstrated that fibre orientation over the cartilage depth has a clear tendency towards native cartilage with increasing time of implantation. After 2 weeks, the collagen fibres of the superficial zone were oriented parallel to the articular surface with little anisotropy present in the middle and deep zones. Overall, fibre orientation and collagen distribution within the implants were less homogenous than in native cartilage tissue. Despite a relatively low number of specimens, the consistent observation of a continuous approximation to native tissue is very promising and suggests that it may not be necessary to engineer the perfect tissue for implantation but rather to provide an intermediate solution to help the body to heal itself.
KW - Animals
KW - Time Factors
KW - Cells, Cultured
KW - Swine
KW - Tissue Engineering/methods
KW - Collagen Type I/metabolism
KW - Cartilage, Articular/cytology/growth & development/metabolism
KW - Chondrocytes/cytology/metabolism/transplantation
KW - Collagen/metabolism
KW - Collagen Type II/metabolism
KW - Femur/cytology/metabolism/surgery
KW - Microscopy, Polarization/methods
KW - Swine, Miniature
KW - Tissue Transplantation/methods
KW - Animals
KW - Time Factors
KW - Cells, Cultured
KW - Swine
KW - Tissue Engineering/methods
KW - Collagen Type I/metabolism
KW - Cartilage, Articular/cytology/growth & development/metabolism
KW - Chondrocytes/cytology/metabolism/transplantation
KW - Collagen/metabolism
KW - Collagen Type II/metabolism
KW - Femur/cytology/metabolism/surgery
KW - Microscopy, Polarization/methods
KW - Swine, Miniature
KW - Tissue Transplantation/methods
M3 - SCORING: Journal article
VL - 23
SP - 209
EP - 221
JO - EUR CELLS MATER
JF - EUR CELLS MATER
SN - 1473-2262
ER -
