The beta-propensity of Tau determines aggregation and synaptic loss in inducible mouse models of tauopathy.
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The beta-propensity of Tau determines aggregation and synaptic loss in inducible mouse models of tauopathy. / Eckermann, Katrin; Mocanu, Maria-Magdalena; Khlistunova, Inna; Biernat, Jacek; Nissen, Astrid; Hofmann, Anne; Schönig, Kai; Bujard, Hermann; Haemisch, Andreas; Mandelkow, Eckhard; Zhou, Lepu; Rune, Gabriele M.; Mandelkow, Eva-Maria.
in: J BIOL CHEM, Jahrgang 282, Nr. 43, 43, 2007, S. 31755-31765.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The beta-propensity of Tau determines aggregation and synaptic loss in inducible mouse models of tauopathy.
AU - Eckermann, Katrin
AU - Mocanu, Maria-Magdalena
AU - Khlistunova, Inna
AU - Biernat, Jacek
AU - Nissen, Astrid
AU - Hofmann, Anne
AU - Schönig, Kai
AU - Bujard, Hermann
AU - Haemisch, Andreas
AU - Mandelkow, Eckhard
AU - Zhou, Lepu
AU - Rune, Gabriele M.
AU - Mandelkow, Eva-Maria
PY - 2007
Y1 - 2007
N2 - Neurofibrillary lesions are characteristic for a group of human diseases, named tauopathies, which are characterized by prominent intracellular accumulations of abnormal filaments formed by the microtubule-associated protein Tau. The tauopathies are accompanied by abnormal changes in Tau protein, including pathological conformation, somatodendritic mislocalization, hyperphosphorylation, and aggregation, whose interdependence is not well understood. To address these issues we have created transgenic mouse lines in which different variants of full-length Tau are expressed in a regulatable fashion, allowing one to switch the expression on and off at defined time points. The Tau variants differ by small mutations in the hexapeptide motifs that control the ability of Tau to adopt a beta-structure conformation and hence to aggregate. The "pro-aggregation" mutant DeltaK280, derived from one of the mutations observed in frontotemporal dementias, aggregates avidly in vitro, whereas the "anti-aggregation" mutant DeltaK280/PP cannot aggregate because of two beta-breaking prolines. In the transgenic mice, the pro-aggregation Tau induces a pathological conformation and pre-tangle aggregation, even at low expression levels, the anti-aggregation mutant does not. This illustrates that abnormal aggregation is primarily controlled by the molecular structure of Tau in vitro and in the organism. Both variants of Tau become mislocalized and hyperphosphorylated independently of aggregation, suggesting that localization and phosphorylation are mainly a consequence of increased concentration. These pathological changes are reversible when the expression of Tau is switched off. The pro-aggregation Tau causes a strong reduction in spine synapses.
AB - Neurofibrillary lesions are characteristic for a group of human diseases, named tauopathies, which are characterized by prominent intracellular accumulations of abnormal filaments formed by the microtubule-associated protein Tau. The tauopathies are accompanied by abnormal changes in Tau protein, including pathological conformation, somatodendritic mislocalization, hyperphosphorylation, and aggregation, whose interdependence is not well understood. To address these issues we have created transgenic mouse lines in which different variants of full-length Tau are expressed in a regulatable fashion, allowing one to switch the expression on and off at defined time points. The Tau variants differ by small mutations in the hexapeptide motifs that control the ability of Tau to adopt a beta-structure conformation and hence to aggregate. The "pro-aggregation" mutant DeltaK280, derived from one of the mutations observed in frontotemporal dementias, aggregates avidly in vitro, whereas the "anti-aggregation" mutant DeltaK280/PP cannot aggregate because of two beta-breaking prolines. In the transgenic mice, the pro-aggregation Tau induces a pathological conformation and pre-tangle aggregation, even at low expression levels, the anti-aggregation mutant does not. This illustrates that abnormal aggregation is primarily controlled by the molecular structure of Tau in vitro and in the organism. Both variants of Tau become mislocalized and hyperphosphorylated independently of aggregation, suggesting that localization and phosphorylation are mainly a consequence of increased concentration. These pathological changes are reversible when the expression of Tau is switched off. The pro-aggregation Tau causes a strong reduction in spine synapses.
M3 - SCORING: Zeitschriftenaufsatz
VL - 282
SP - 31755
EP - 31765
JO - J BIOL CHEM
JF - J BIOL CHEM
SN - 0021-9258
IS - 43
M1 - 43
ER -