The AN69 hemofiltration membrane has a decreasing effect on the intracellular diadenosine pentaphosphate concentration of platelets
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The AN69 hemofiltration membrane has a decreasing effect on the intracellular diadenosine pentaphosphate concentration of platelets. / Jankowski, Joachim; Schlüter, Hartmut; Henning, Lars; van der Giet, Markus; Jankowski, Vera; Zidek, Walter; Tepel, Martin.
in: KIDNEY BLOOD PRESS R, Jahrgang 26, Nr. 1, 2003, S. 50-4.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The AN69 hemofiltration membrane has a decreasing effect on the intracellular diadenosine pentaphosphate concentration of platelets
AU - Jankowski, Joachim
AU - Schlüter, Hartmut
AU - Henning, Lars
AU - van der Giet, Markus
AU - Jankowski, Vera
AU - Zidek, Walter
AU - Tepel, Martin
N1 - Copyright 2003 S. Karger AG, Basel
PY - 2003
Y1 - 2003
N2 - BACKGROUND/AIM: The biocompatibility of hemodialysis membranes has a substantial impact on the mortality of patients with end-stage renal failure. In the present study, the effects of hemodialysis on the intracellular amount of diadenosine pentaphosphate (Ap(5)A), a hydrophilic, anionic substance with a low molecular weight, was investigated.METHODS: The intracellular Ap(5)A concentrations were measured before and after hemodialysis using either polyacrylonitrile (AN69; n = 10) or polysulfone (n = 23) membranes. Ap(5)A was isolated from platelets using affinity chromatography and reversed-phase chromatography methods.RESULTS: The Ap(5)A concentrations were quantified by ultraviolet absorption at 254 nm. The Ap(5)A concentrations were significantly higher in platelets from the patients with end-stage renal failure as compared with the 21 healthy control subjects (136 +/- 50 vs. 9 +/- 6 fg/platelet; mean +/- SEM, p < 0.01). Before hemodialysis, the intracellular Ap(5)A concentrations in platelets from 10 patients with end-stage renal failure using an AN69 membrane were not significantly different from those in platelets from 23 patients using a polysulfone membrane (93 +/- 39 vs. 155 +/- 70 fg/platelet). However, after a hemodialysis session, the intracellular Ap(5)A concentrations in platelets from patients with end-stage renal failure using an AN69 membrane were significantly lower as compared with those in platelets before hemodialysis (51 +/- 18 vs. 93 +/- 39 fg/platelet, p < 0.05) as well as compared with those in platelets from patients using a polysulfone membrane (51 +/- 18 vs. 250 +/- 59 fg/platelet, p < 0.05).CONCLUSIONS: It was found that hemofiltration by using an AN69 membrane has a direct effect on the intracellular amount of Ap(5)A and that changes of intracellular hydrophilic substances are dependent on the hemodialysis membrane used.
AB - BACKGROUND/AIM: The biocompatibility of hemodialysis membranes has a substantial impact on the mortality of patients with end-stage renal failure. In the present study, the effects of hemodialysis on the intracellular amount of diadenosine pentaphosphate (Ap(5)A), a hydrophilic, anionic substance with a low molecular weight, was investigated.METHODS: The intracellular Ap(5)A concentrations were measured before and after hemodialysis using either polyacrylonitrile (AN69; n = 10) or polysulfone (n = 23) membranes. Ap(5)A was isolated from platelets using affinity chromatography and reversed-phase chromatography methods.RESULTS: The Ap(5)A concentrations were quantified by ultraviolet absorption at 254 nm. The Ap(5)A concentrations were significantly higher in platelets from the patients with end-stage renal failure as compared with the 21 healthy control subjects (136 +/- 50 vs. 9 +/- 6 fg/platelet; mean +/- SEM, p < 0.01). Before hemodialysis, the intracellular Ap(5)A concentrations in platelets from 10 patients with end-stage renal failure using an AN69 membrane were not significantly different from those in platelets from 23 patients using a polysulfone membrane (93 +/- 39 vs. 155 +/- 70 fg/platelet). However, after a hemodialysis session, the intracellular Ap(5)A concentrations in platelets from patients with end-stage renal failure using an AN69 membrane were significantly lower as compared with those in platelets before hemodialysis (51 +/- 18 vs. 93 +/- 39 fg/platelet, p < 0.05) as well as compared with those in platelets from patients using a polysulfone membrane (51 +/- 18 vs. 250 +/- 59 fg/platelet, p < 0.05).CONCLUSIONS: It was found that hemofiltration by using an AN69 membrane has a direct effect on the intracellular amount of Ap(5)A and that changes of intracellular hydrophilic substances are dependent on the hemodialysis membrane used.
KW - Acrylic Resins
KW - Blood Platelets
KW - Dinucleoside Phosphates
KW - Female
KW - Hemofiltration
KW - Humans
KW - Kidney Failure, Chronic
KW - Male
KW - Membranes, Artificial
KW - Middle Aged
KW - Polymers
KW - Renal Dialysis
KW - Sulfones
KW - Clinical Trial
KW - Comparative Study
KW - Journal Article
U2 - 10.1159/000069765
DO - 10.1159/000069765
M3 - SCORING: Journal article
C2 - 12697977
VL - 26
SP - 50
EP - 54
JO - KIDNEY BLOOD PRESS R
JF - KIDNEY BLOOD PRESS R
SN - 1420-4096
IS - 1
ER -
