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Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

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Tempol protects against intravitreous indocyanine green-induced retinal damage in rats. / Thaler, Sebastian; Voykov, Bogomil; Willmann, Gabriel; Fiedorowicz, Michal; Rejdak, Robert; Gekeler, Florian; May, C Albrecht; Schatz, Andreas; Schuettauf, Frank.

in: GRAEF ARCH CLIN EXP, Jahrgang 250, Nr. 11, 11.2012, S. 1597-606.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Thaler, S, Voykov, B, Willmann, G, Fiedorowicz, M, Rejdak, R, Gekeler, F, May, CA, Schatz, A & Schuettauf, F 2012, 'Tempol protects against intravitreous indocyanine green-induced retinal damage in rats', GRAEF ARCH CLIN EXP, Jg. 250, Nr. 11, S. 1597-606. https://doi.org/10.1007/s00417-012-2000-1

APA

Thaler, S., Voykov, B., Willmann, G., Fiedorowicz, M., Rejdak, R., Gekeler, F., May, C. A., Schatz, A., & Schuettauf, F. (2012). Tempol protects against intravitreous indocyanine green-induced retinal damage in rats. GRAEF ARCH CLIN EXP, 250(11), 1597-606. https://doi.org/10.1007/s00417-012-2000-1

Vancouver

Thaler S, Voykov B, Willmann G, Fiedorowicz M, Rejdak R, Gekeler F et al. Tempol protects against intravitreous indocyanine green-induced retinal damage in rats. GRAEF ARCH CLIN EXP. 2012 Nov;250(11):1597-606. https://doi.org/10.1007/s00417-012-2000-1

Bibtex

@article{73825f8a4368495ea561366d377b4e42,
title = "Tempol protects against intravitreous indocyanine green-induced retinal damage in rats",
abstract = "PURPOSE: Indocyanine green (ICG) has been widely used as a vital dye for macular surgery. However, ICG can be toxic to retinal cells. Here we evaluate whether tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a free radical scavenger, can protect against ICG-induced retinal damage in rats.METHODS: Brown Norway rats received intravitreal injections of ICG 0.5 % or BSS as controls. Tempol (20 mg/kg BW) or PBS as a control was administered intraperitoneally 24 h and 30 min before ICG and once daily for 7 consecutive days. Tempol was detected in the retina using electron paramagnetic resonance (EPR) spectroscopy. One week after ICG injections, the effects of tempol on retinal toxicity were assessed by retinal ganglion cell (RGC) back-labeling and by light microscopy. Electroretinography (ERG) was performed after 1 and 2 weeks.RESULTS: ICG administration reduced RGC numbers by 17 % (1,943 ± 45 vs. 2,342 ± 31 RGCs/mm(2)). Tempol treatment rescued RGCs in a significant manner (2,258 ± 36, p < 0.01) and diminished morphological changes detected by light microscopy. ICG-injected eyes showed a significant reduction of ERG potentials only in PBS-treated animals (V(max) 530 ± 145 µV vs. 779 ± 179 µV, p = 0.0052), but not in the tempol-treated group.CONCLUSIONS: Tempol significantly attenuates ICG-induced toxicity in rat retinas and may therefore be considered for further evaluation as accompanying treatment in ICG-assisted chromovitrectomy.",
keywords = "Animals, Cell Count, Cell Survival, Coloring Agents/toxicity, Cyclic N-Oxides/pharmacology, Dark Adaptation, Electron Spin Resonance Spectroscopy, Electroretinography, Female, Free Radical Scavengers/pharmacology, Indocyanine Green/toxicity, Intravitreal Injections, Neuroprotective Agents/pharmacology, Photic Stimulation, Rats, Rats, Inbred BN, Retinal Cone Photoreceptor Cells/physiology, Retinal Diseases/chemically induced, Retinal Ganglion Cells/drug effects, Spin Labels",
author = "Sebastian Thaler and Bogomil Voykov and Gabriel Willmann and Michal Fiedorowicz and Robert Rejdak and Florian Gekeler and May, {C Albrecht} and Andreas Schatz and Frank Schuettauf",
year = "2012",
month = nov,
doi = "10.1007/s00417-012-2000-1",
language = "English",
volume = "250",
pages = "1597--606",
journal = "GRAEF ARCH CLIN EXP",
issn = "0721-832X",
publisher = "Springer",
number = "11",

}

RIS

TY - JOUR

T1 - Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

AU - Thaler, Sebastian

AU - Voykov, Bogomil

AU - Willmann, Gabriel

AU - Fiedorowicz, Michal

AU - Rejdak, Robert

AU - Gekeler, Florian

AU - May, C Albrecht

AU - Schatz, Andreas

AU - Schuettauf, Frank

PY - 2012/11

Y1 - 2012/11

N2 - PURPOSE: Indocyanine green (ICG) has been widely used as a vital dye for macular surgery. However, ICG can be toxic to retinal cells. Here we evaluate whether tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a free radical scavenger, can protect against ICG-induced retinal damage in rats.METHODS: Brown Norway rats received intravitreal injections of ICG 0.5 % or BSS as controls. Tempol (20 mg/kg BW) or PBS as a control was administered intraperitoneally 24 h and 30 min before ICG and once daily for 7 consecutive days. Tempol was detected in the retina using electron paramagnetic resonance (EPR) spectroscopy. One week after ICG injections, the effects of tempol on retinal toxicity were assessed by retinal ganglion cell (RGC) back-labeling and by light microscopy. Electroretinography (ERG) was performed after 1 and 2 weeks.RESULTS: ICG administration reduced RGC numbers by 17 % (1,943 ± 45 vs. 2,342 ± 31 RGCs/mm(2)). Tempol treatment rescued RGCs in a significant manner (2,258 ± 36, p < 0.01) and diminished morphological changes detected by light microscopy. ICG-injected eyes showed a significant reduction of ERG potentials only in PBS-treated animals (V(max) 530 ± 145 µV vs. 779 ± 179 µV, p = 0.0052), but not in the tempol-treated group.CONCLUSIONS: Tempol significantly attenuates ICG-induced toxicity in rat retinas and may therefore be considered for further evaluation as accompanying treatment in ICG-assisted chromovitrectomy.

AB - PURPOSE: Indocyanine green (ICG) has been widely used as a vital dye for macular surgery. However, ICG can be toxic to retinal cells. Here we evaluate whether tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a free radical scavenger, can protect against ICG-induced retinal damage in rats.METHODS: Brown Norway rats received intravitreal injections of ICG 0.5 % or BSS as controls. Tempol (20 mg/kg BW) or PBS as a control was administered intraperitoneally 24 h and 30 min before ICG and once daily for 7 consecutive days. Tempol was detected in the retina using electron paramagnetic resonance (EPR) spectroscopy. One week after ICG injections, the effects of tempol on retinal toxicity were assessed by retinal ganglion cell (RGC) back-labeling and by light microscopy. Electroretinography (ERG) was performed after 1 and 2 weeks.RESULTS: ICG administration reduced RGC numbers by 17 % (1,943 ± 45 vs. 2,342 ± 31 RGCs/mm(2)). Tempol treatment rescued RGCs in a significant manner (2,258 ± 36, p < 0.01) and diminished morphological changes detected by light microscopy. ICG-injected eyes showed a significant reduction of ERG potentials only in PBS-treated animals (V(max) 530 ± 145 µV vs. 779 ± 179 µV, p = 0.0052), but not in the tempol-treated group.CONCLUSIONS: Tempol significantly attenuates ICG-induced toxicity in rat retinas and may therefore be considered for further evaluation as accompanying treatment in ICG-assisted chromovitrectomy.

KW - Animals

KW - Cell Count

KW - Cell Survival

KW - Coloring Agents/toxicity

KW - Cyclic N-Oxides/pharmacology

KW - Dark Adaptation

KW - Electron Spin Resonance Spectroscopy

KW - Electroretinography

KW - Female

KW - Free Radical Scavengers/pharmacology

KW - Indocyanine Green/toxicity

KW - Intravitreal Injections

KW - Neuroprotective Agents/pharmacology

KW - Photic Stimulation

KW - Rats

KW - Rats, Inbred BN

KW - Retinal Cone Photoreceptor Cells/physiology

KW - Retinal Diseases/chemically induced

KW - Retinal Ganglion Cells/drug effects

KW - Spin Labels

U2 - 10.1007/s00417-012-2000-1

DO - 10.1007/s00417-012-2000-1

M3 - SCORING: Journal article

C2 - 22460632

VL - 250

SP - 1597

EP - 1606

JO - GRAEF ARCH CLIN EXP

JF - GRAEF ARCH CLIN EXP

SN - 0721-832X

IS - 11

ER -