Telomerase-deficient mice exhibit bone loss owing to defects in osteoblasts and increased osteoclastogenesis by inflammatory microenvironment.
Standard
Telomerase-deficient mice exhibit bone loss owing to defects in osteoblasts and increased osteoclastogenesis by inflammatory microenvironment. / Saeed, Hamid; Abdallah, Basem M; Ditzel, Nicholas; Catalá-Lehnen, Philip; Qiu, Weimin; Amling, Michael; Kassem, Moustapha.
in: J BONE MINER RES, Jahrgang 26, Nr. 7, 7, 2011, S. 1494-1505.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Telomerase-deficient mice exhibit bone loss owing to defects in osteoblasts and increased osteoclastogenesis by inflammatory microenvironment.
AU - Saeed, Hamid
AU - Abdallah, Basem M
AU - Ditzel, Nicholas
AU - Catalá-Lehnen, Philip
AU - Qiu, Weimin
AU - Amling, Michael
AU - Kassem, Moustapha
PY - 2011
Y1 - 2011
N2 - Telomere shortening owing to telomerase deficiency leads to accelerated senescence of human skeletal (mesenchymal) stem cells (MSCs) in vitro, whereas overexpression leads to telomere elongation, extended life span, and enhanced bone formation. To study the role of telomere shortening in vivo, we studied the phenotype of telomerase-deficient mice (Terc(-/-)). Terc(-/-) mice exhibited accelerated age-related bone loss starting at 3 months of age and during 12 months of follow-up revealed by dual-energy X-ray absorptiometric (DXA) scanning and by micro-computed tomography (µCT). Bone histomorphometry revealed decreased mineralized surface and bone-formation rate as well as increased osteoclast number and size in Terc(-/-) mice. Also, serum total deoxypyridinoline (tDPD) was increased in Terc(-/-) mice. MSCs and osteoprogenitors isolated from Terc(-/-) mice exhibited intrinsic defects with reduced proliferating cell number and impaired osteogenic differentiation capacity. In addition, the Terc(-/-) -MSC cultures accumulated a larger proportion of senescent ?-galactosidase(+) cells and cells exhibiting DNA damage. Microarray analysis of Terc(-/-) bone revealed significant overexpression of a large number of proinflammatory genes involved in osteoclast (OC) differentiation. Consistently, serum obtained from Terc(-/-) mice enhanced OC formation of wild-type bone marrow cultures. Our data demonstrate two mechanisms for age-related bone loss caused by telomerase deficiency: intrinsic osteoblastic defects and creation of a proinflammatory osteoclast-activating microenvironment. Thus telomerization of MSCs may provide a novel approach for abolishing age-related bone loss.
AB - Telomere shortening owing to telomerase deficiency leads to accelerated senescence of human skeletal (mesenchymal) stem cells (MSCs) in vitro, whereas overexpression leads to telomere elongation, extended life span, and enhanced bone formation. To study the role of telomere shortening in vivo, we studied the phenotype of telomerase-deficient mice (Terc(-/-)). Terc(-/-) mice exhibited accelerated age-related bone loss starting at 3 months of age and during 12 months of follow-up revealed by dual-energy X-ray absorptiometric (DXA) scanning and by micro-computed tomography (µCT). Bone histomorphometry revealed decreased mineralized surface and bone-formation rate as well as increased osteoclast number and size in Terc(-/-) mice. Also, serum total deoxypyridinoline (tDPD) was increased in Terc(-/-) mice. MSCs and osteoprogenitors isolated from Terc(-/-) mice exhibited intrinsic defects with reduced proliferating cell number and impaired osteogenic differentiation capacity. In addition, the Terc(-/-) -MSC cultures accumulated a larger proportion of senescent ?-galactosidase(+) cells and cells exhibiting DNA damage. Microarray analysis of Terc(-/-) bone revealed significant overexpression of a large number of proinflammatory genes involved in osteoclast (OC) differentiation. Consistently, serum obtained from Terc(-/-) mice enhanced OC formation of wild-type bone marrow cultures. Our data demonstrate two mechanisms for age-related bone loss caused by telomerase deficiency: intrinsic osteoblastic defects and creation of a proinflammatory osteoclast-activating microenvironment. Thus telomerization of MSCs may provide a novel approach for abolishing age-related bone loss.
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Mice, Inbred C57BL
KW - Models, Biological
KW - Phenotype
KW - Gene Expression Profiling
KW - Cell Proliferation
KW - Cell Differentiation
KW - Cell Aging
KW - Bone Marrow Cells/metabolism
KW - Adipocytes/metabolism/pathology
KW - Bone and Bones/pathology/radiography
KW - Inflammation/complications/genetics/pathology
KW - Mesenchymal Stem Cells/metabolism/pathology
KW - Osteoblasts/metabolism/pathology
KW - Osteoclasts/metabolism/pathology
KW - Osteogenesis
KW - Osteoporosis/complications/pathology/radiography
KW - Telomerase/blood/deficiency/metabolism
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Mice, Inbred C57BL
KW - Models, Biological
KW - Phenotype
KW - Gene Expression Profiling
KW - Cell Proliferation
KW - Cell Differentiation
KW - Cell Aging
KW - Bone Marrow Cells/metabolism
KW - Adipocytes/metabolism/pathology
KW - Bone and Bones/pathology/radiography
KW - Inflammation/complications/genetics/pathology
KW - Mesenchymal Stem Cells/metabolism/pathology
KW - Osteoblasts/metabolism/pathology
KW - Osteoclasts/metabolism/pathology
KW - Osteogenesis
KW - Osteoporosis/complications/pathology/radiography
KW - Telomerase/blood/deficiency/metabolism
M3 - SCORING: Journal article
VL - 26
SP - 1494
EP - 1505
JO - J BONE MINER RES
JF - J BONE MINER RES
SN - 0884-0431
IS - 7
M1 - 7
ER -