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Taste responses in mice lacking taste receptor subunit T1R1

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Taste responses in mice lacking taste receptor subunit T1R1. / Kusuhara, Yoko; Yoshida, Ryusuke; Ohkuri, Tadahiro; Yasumatsu, Keiko; Voigt, Anja; Hübner, Sandra; Maeda, Katsumasa; Boehm, Ulrich; Meyerhof, Wolfgang; Ninomiya, Yuzo.

in: J PHYSIOL-LONDON, Jahrgang 591, Nr. Pt 7, 01.04.2013, S. 1967-85.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kusuhara, Y, Yoshida, R, Ohkuri, T, Yasumatsu, K, Voigt, A, Hübner, S, Maeda, K, Boehm, U, Meyerhof, W & Ninomiya, Y 2013, 'Taste responses in mice lacking taste receptor subunit T1R1', J PHYSIOL-LONDON, Jg. 591, Nr. Pt 7, S. 1967-85. https://doi.org/10.1113/jphysiol.2012.236604

APA

Kusuhara, Y., Yoshida, R., Ohkuri, T., Yasumatsu, K., Voigt, A., Hübner, S., Maeda, K., Boehm, U., Meyerhof, W., & Ninomiya, Y. (2013). Taste responses in mice lacking taste receptor subunit T1R1. J PHYSIOL-LONDON, 591(Pt 7), 1967-85. https://doi.org/10.1113/jphysiol.2012.236604

Vancouver

Kusuhara Y, Yoshida R, Ohkuri T, Yasumatsu K, Voigt A, Hübner S et al. Taste responses in mice lacking taste receptor subunit T1R1. J PHYSIOL-LONDON. 2013 Apr 1;591(Pt 7):1967-85. https://doi.org/10.1113/jphysiol.2012.236604

Bibtex

@article{fa1bfab72a9d4aa1949c53db58bdbcd9,
title = "Taste responses in mice lacking taste receptor subunit T1R1",
abstract = "The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1(-/-)) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1(-/-) mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1(-/-) mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1(+/-) mice responded to sweet and umami compounds, whereas those in T1R1(-/-) mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1(-/-) than in T1R1(+/-) mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1(-/-) and T1R1(+/-) mice. Conditioned taste aversion tests demonstrated that both T1R1(-/-) and T1R1(+/-) mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.",
keywords = "Animals, Behavior, Animal, Chorda Tympani Nerve, Female, Glossopharyngeal Nerve, Glutamic Acid, Male, Mice, Mice, Transgenic, Protein Subunits, Receptors, G-Protein-Coupled, Receptors, Metabotropic Glutamate, Taste, Taste Buds",
author = "Yoko Kusuhara and Ryusuke Yoshida and Tadahiro Ohkuri and Keiko Yasumatsu and Anja Voigt and Sandra H{\"u}bner and Katsumasa Maeda and Ulrich Boehm and Wolfgang Meyerhof and Yuzo Ninomiya",
year = "2013",
month = apr,
day = "1",
doi = "10.1113/jphysiol.2012.236604",
language = "English",
volume = "591",
pages = "1967--85",
journal = "J PHYSIOL-LONDON",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "Pt 7",

}

RIS

TY - JOUR

T1 - Taste responses in mice lacking taste receptor subunit T1R1

AU - Kusuhara, Yoko

AU - Yoshida, Ryusuke

AU - Ohkuri, Tadahiro

AU - Yasumatsu, Keiko

AU - Voigt, Anja

AU - Hübner, Sandra

AU - Maeda, Katsumasa

AU - Boehm, Ulrich

AU - Meyerhof, Wolfgang

AU - Ninomiya, Yuzo

PY - 2013/4/1

Y1 - 2013/4/1

N2 - The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1(-/-)) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1(-/-) mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1(-/-) mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1(+/-) mice responded to sweet and umami compounds, whereas those in T1R1(-/-) mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1(-/-) than in T1R1(+/-) mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1(-/-) and T1R1(+/-) mice. Conditioned taste aversion tests demonstrated that both T1R1(-/-) and T1R1(+/-) mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.

AB - The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1(-/-)) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1(-/-) mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1(-/-) mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1(+/-) mice responded to sweet and umami compounds, whereas those in T1R1(-/-) mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1(-/-) than in T1R1(+/-) mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1(-/-) and T1R1(+/-) mice. Conditioned taste aversion tests demonstrated that both T1R1(-/-) and T1R1(+/-) mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.

KW - Animals

KW - Behavior, Animal

KW - Chorda Tympani Nerve

KW - Female

KW - Glossopharyngeal Nerve

KW - Glutamic Acid

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - Protein Subunits

KW - Receptors, G-Protein-Coupled

KW - Receptors, Metabotropic Glutamate

KW - Taste

KW - Taste Buds

U2 - 10.1113/jphysiol.2012.236604

DO - 10.1113/jphysiol.2012.236604

M3 - SCORING: Journal article

C2 - 23339178

VL - 591

SP - 1967

EP - 1985

JO - J PHYSIOL-LONDON

JF - J PHYSIOL-LONDON

SN - 0022-3751

IS - Pt 7

ER -