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Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection

Standard

Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection. / Yao, Virginia J; Ozawa, Michael G; Trepel, Martin; Arap, Wadih; McDonald, Donald M; Pasqualini, Renata.

in: AM J PATHOL, Jahrgang 166, Nr. 2, 02.2005, S. 625-36.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Yao, VJ, Ozawa, MG, Trepel, M, Arap, W, McDonald, DM & Pasqualini, R 2005, 'Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection', AM J PATHOL, Jg. 166, Nr. 2, S. 625-36. https://doi.org/10.1016/S0002-9440(10)62283-3

APA

Yao, V. J., Ozawa, M. G., Trepel, M., Arap, W., McDonald, D. M., & Pasqualini, R. (2005). Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection. AM J PATHOL, 166(2), 625-36. https://doi.org/10.1016/S0002-9440(10)62283-3

Vancouver

Yao VJ, Ozawa MG, Trepel M, Arap W, McDonald DM, Pasqualini R. Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection. AM J PATHOL. 2005 Feb;166(2):625-36. https://doi.org/10.1016/S0002-9440(10)62283-3

Bibtex

@article{484c3230fed84fc8b6eec36c3abf2038,
title = "Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection",
abstract = "Heterogeneity of the microvasculature in different organs has been well documented by multiple methods including in vivo phage display. However, less is known about the diversity of blood vessels within functionally distinct regions of organs. Here, we combined in vivo phage display with laser pressure catapult microdissection to identify peptide ligands for vascular receptors in the islets of Langerhans in the murine pancreas. Protein database analyses of the peptides, CVSNPRWKC and CHVLWSTRC, showed sequence identity to two ephrin A-type ligand homologues, A2 and A4. Confocal microscopy confirmed that most immunoreactivity of CVSNPRWKC and CHVLWSTRC phage was associated with blood vessels in pancreatic islets. Antibodies recognizing EphA4, a receptor for ephrin-A ligands, were similarly associated with islet blood vessels. Importantly, binding of both islet-homing phage and anti-EphA4 antibody was strikingly increased in blood vessels of pancreatic islet tumors in RIP-Tag2 transgenic mice. These results indicate that endothelial cells of blood vessels in pancreatic islets preferentially express EphA4 receptors, and this expression is increased in tumors. Our findings show in vivo phage display and laser pressure catapult microdissection can be combined to reveal endothelial cell specialization within focal regions of the microvasculature.",
keywords = "Amino Acid Sequence, Animals, Antigens, CD31, Clinical Trials as Topic, Databases as Topic, Dissection, Ephrin-A1, Humans, Immunohistochemistry, Islets of Langerhans, Lasers, Ligands, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microcirculation, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Peptide Library, Peptides, Protein Structure, Secondary, Software, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.",
author = "Yao, {Virginia J} and Ozawa, {Michael G} and Martin Trepel and Wadih Arap and McDonald, {Donald M} and Renata Pasqualini",
year = "2005",
month = feb,
doi = "10.1016/S0002-9440(10)62283-3",
language = "English",
volume = "166",
pages = "625--36",
journal = "AM J PATHOL",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection

AU - Yao, Virginia J

AU - Ozawa, Michael G

AU - Trepel, Martin

AU - Arap, Wadih

AU - McDonald, Donald M

AU - Pasqualini, Renata

PY - 2005/2

Y1 - 2005/2

N2 - Heterogeneity of the microvasculature in different organs has been well documented by multiple methods including in vivo phage display. However, less is known about the diversity of blood vessels within functionally distinct regions of organs. Here, we combined in vivo phage display with laser pressure catapult microdissection to identify peptide ligands for vascular receptors in the islets of Langerhans in the murine pancreas. Protein database analyses of the peptides, CVSNPRWKC and CHVLWSTRC, showed sequence identity to two ephrin A-type ligand homologues, A2 and A4. Confocal microscopy confirmed that most immunoreactivity of CVSNPRWKC and CHVLWSTRC phage was associated with blood vessels in pancreatic islets. Antibodies recognizing EphA4, a receptor for ephrin-A ligands, were similarly associated with islet blood vessels. Importantly, binding of both islet-homing phage and anti-EphA4 antibody was strikingly increased in blood vessels of pancreatic islet tumors in RIP-Tag2 transgenic mice. These results indicate that endothelial cells of blood vessels in pancreatic islets preferentially express EphA4 receptors, and this expression is increased in tumors. Our findings show in vivo phage display and laser pressure catapult microdissection can be combined to reveal endothelial cell specialization within focal regions of the microvasculature.

AB - Heterogeneity of the microvasculature in different organs has been well documented by multiple methods including in vivo phage display. However, less is known about the diversity of blood vessels within functionally distinct regions of organs. Here, we combined in vivo phage display with laser pressure catapult microdissection to identify peptide ligands for vascular receptors in the islets of Langerhans in the murine pancreas. Protein database analyses of the peptides, CVSNPRWKC and CHVLWSTRC, showed sequence identity to two ephrin A-type ligand homologues, A2 and A4. Confocal microscopy confirmed that most immunoreactivity of CVSNPRWKC and CHVLWSTRC phage was associated with blood vessels in pancreatic islets. Antibodies recognizing EphA4, a receptor for ephrin-A ligands, were similarly associated with islet blood vessels. Importantly, binding of both islet-homing phage and anti-EphA4 antibody was strikingly increased in blood vessels of pancreatic islet tumors in RIP-Tag2 transgenic mice. These results indicate that endothelial cells of blood vessels in pancreatic islets preferentially express EphA4 receptors, and this expression is increased in tumors. Our findings show in vivo phage display and laser pressure catapult microdissection can be combined to reveal endothelial cell specialization within focal regions of the microvasculature.

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, CD31

KW - Clinical Trials as Topic

KW - Databases as Topic

KW - Dissection

KW - Ephrin-A1

KW - Humans

KW - Immunohistochemistry

KW - Islets of Langerhans

KW - Lasers

KW - Ligands

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Microcirculation

KW - Microscopy, Confocal

KW - Microscopy, Fluorescence

KW - Molecular Sequence Data

KW - Peptide Library

KW - Peptides

KW - Protein Structure, Secondary

KW - Software

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

U2 - 10.1016/S0002-9440(10)62283-3

DO - 10.1016/S0002-9440(10)62283-3

M3 - SCORING: Journal article

C2 - 15681844

VL - 166

SP - 625

EP - 636

JO - AM J PATHOL

JF - AM J PATHOL

SN - 0002-9440

IS - 2

ER -