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Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice

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Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice. / Simsekyilmaz, Sakine; Liehn, Elisa A; Weinandy, Stefan; Schreiber, Fabian; Megens, Remco T A; Theelen, Wendy; Smeets, Ralf; Jockenhövel, Stefan; Gries, Thomas; Möller, Martin; Klee, Doris; Weber, Christian; Zernecke, Alma.

in: PLOS ONE, Jahrgang 11, Nr. 5, 18.05.2016, S. e0155829.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Simsekyilmaz, S, Liehn, EA, Weinandy, S, Schreiber, F, Megens, RTA, Theelen, W, Smeets, R, Jockenhövel, S, Gries, T, Möller, M, Klee, D, Weber, C & Zernecke, A 2016, 'Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice', PLOS ONE, Jg. 11, Nr. 5, S. e0155829. https://doi.org/10.1371/journal.pone.0155829

APA

Simsekyilmaz, S., Liehn, E. A., Weinandy, S., Schreiber, F., Megens, R. T. A., Theelen, W., Smeets, R., Jockenhövel, S., Gries, T., Möller, M., Klee, D., Weber, C., & Zernecke, A. (2016). Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice. PLOS ONE, 11(5), e0155829. https://doi.org/10.1371/journal.pone.0155829

Vancouver

Simsekyilmaz S, Liehn EA, Weinandy S, Schreiber F, Megens RTA, Theelen W et al. Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice. PLOS ONE. 2016 Mai 18;11(5):e0155829. https://doi.org/10.1371/journal.pone.0155829

Bibtex

@article{d4e58bab190f495689293a05de787168,
title = "Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice",
abstract = "Atherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, or RGD/CXCL1. Cell adhesion to star-PEG-coated nitinol foils was unaltered or reduced, whereas bio-functionalization with RGD but foremost RGD/CXCL1 increased adhesion of early angiogenic outgrowth cells (EOCs) and endothelial cells but not smooth muscle cells when compared with bare metal foils. Stimulation of cells with RGD/CXCL1 furthermore increased the proliferation of EOCs. In vivo, bio-functionalization with RGD/CXCL1 significantly reduced neointima formation and thrombus formation, and increased re-endothelialization in apoE-/- carotid arteries compared with bare-metal nitinol stents, star-PEG-coated stents, and stents bio-functionalized with RGD only. Bio-functionalization of star-PEG-coated nitinol-stents with RGD/CXCL1 reduced in-stent neointima formation. By supporting the adhesion and proliferation of endothelial progenitor cells, RGD/CXCL1 coating of stents may help to accelerate endothelial repair after stent implantation, and thus may harbor the potential to limit the complication of in-stent restenosis in clinical approaches.",
author = "Sakine Simsekyilmaz and Liehn, {Elisa A} and Stefan Weinandy and Fabian Schreiber and Megens, {Remco T A} and Wendy Theelen and Ralf Smeets and Stefan Jockenh{\"o}vel and Thomas Gries and Martin M{\"o}ller and Doris Klee and Christian Weber and Alma Zernecke",
year = "2016",
month = may,
day = "18",
doi = "10.1371/journal.pone.0155829",
language = "English",
volume = "11",
pages = "e0155829",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice

AU - Simsekyilmaz, Sakine

AU - Liehn, Elisa A

AU - Weinandy, Stefan

AU - Schreiber, Fabian

AU - Megens, Remco T A

AU - Theelen, Wendy

AU - Smeets, Ralf

AU - Jockenhövel, Stefan

AU - Gries, Thomas

AU - Möller, Martin

AU - Klee, Doris

AU - Weber, Christian

AU - Zernecke, Alma

PY - 2016/5/18

Y1 - 2016/5/18

N2 - Atherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, or RGD/CXCL1. Cell adhesion to star-PEG-coated nitinol foils was unaltered or reduced, whereas bio-functionalization with RGD but foremost RGD/CXCL1 increased adhesion of early angiogenic outgrowth cells (EOCs) and endothelial cells but not smooth muscle cells when compared with bare metal foils. Stimulation of cells with RGD/CXCL1 furthermore increased the proliferation of EOCs. In vivo, bio-functionalization with RGD/CXCL1 significantly reduced neointima formation and thrombus formation, and increased re-endothelialization in apoE-/- carotid arteries compared with bare-metal nitinol stents, star-PEG-coated stents, and stents bio-functionalized with RGD only. Bio-functionalization of star-PEG-coated nitinol-stents with RGD/CXCL1 reduced in-stent neointima formation. By supporting the adhesion and proliferation of endothelial progenitor cells, RGD/CXCL1 coating of stents may help to accelerate endothelial repair after stent implantation, and thus may harbor the potential to limit the complication of in-stent restenosis in clinical approaches.

AB - Atherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, or RGD/CXCL1. Cell adhesion to star-PEG-coated nitinol foils was unaltered or reduced, whereas bio-functionalization with RGD but foremost RGD/CXCL1 increased adhesion of early angiogenic outgrowth cells (EOCs) and endothelial cells but not smooth muscle cells when compared with bare metal foils. Stimulation of cells with RGD/CXCL1 furthermore increased the proliferation of EOCs. In vivo, bio-functionalization with RGD/CXCL1 significantly reduced neointima formation and thrombus formation, and increased re-endothelialization in apoE-/- carotid arteries compared with bare-metal nitinol stents, star-PEG-coated stents, and stents bio-functionalized with RGD only. Bio-functionalization of star-PEG-coated nitinol-stents with RGD/CXCL1 reduced in-stent neointima formation. By supporting the adhesion and proliferation of endothelial progenitor cells, RGD/CXCL1 coating of stents may help to accelerate endothelial repair after stent implantation, and thus may harbor the potential to limit the complication of in-stent restenosis in clinical approaches.

U2 - 10.1371/journal.pone.0155829

DO - 10.1371/journal.pone.0155829

M3 - SCORING: Journal article

C2 - 27192172

VL - 11

SP - e0155829

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 5

ER -