Targeting gene expression during the early bone healing period in the mandible: A base for bone tissue engineering
Standard
Targeting gene expression during the early bone healing period in the mandible: A base for bone tissue engineering. / Beck-Broichsitter, Benedicta E; Werk, Anneke N; Smeets, Ralf; Gröbe, Alexander; Heiland, Max; Cascorbi, Ingolf; Wiltfang, Jörg; Häsler, Robert; Becker, Stephan T.
in: J CRANIO MAXILL SURG, Jahrgang 43, Nr. 8, 10.2015, S. 1452-1460.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Targeting gene expression during the early bone healing period in the mandible: A base for bone tissue engineering
AU - Beck-Broichsitter, Benedicta E
AU - Werk, Anneke N
AU - Smeets, Ralf
AU - Gröbe, Alexander
AU - Heiland, Max
AU - Cascorbi, Ingolf
AU - Wiltfang, Jörg
AU - Häsler, Robert
AU - Becker, Stephan T
N1 - Copyright © 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
PY - 2015/10
Y1 - 2015/10
N2 - PURPOSE: Although bone tissue engineering techniques have become more and more sophisticated than in the past, natural bone healing mechanisms have not been sufficiently considered for further improvement of these techniques so far. We used an established animal model with transcriptome analysis to generate an unbiased picture of early bone healing to support tissue engineering concepts.MATERIAL AND METHODS: In 30 Wistar rats, a 3-mm bone defect was created in the mandibular angle. Tissue was sampled at 5, 10, and 15 days, and the former defect area was excised to undergo transcriptome analysis after RNA extraction. Five differentially expressed genes were further evaluated with reverse transcription-polymerase chain reaction (rt-PCR).RESULTS: Transcriptome analysis revealed 2467 significantly over- and under-expressed transcripts after 5 days and 2265 after 15 days of bone healing, respectively. Validation via rt-PCR confirmed overexpression of osteoactivin, angiopoietin-like factor-4, and metallomatrix proteinase-9 and underexpression of mastcellprotease-10 and proteoglycane-2 in comparison to values in the control group.CONCLUSION: This systematic genome-wide transcriptome analysis helps to decipher the physiological mechanisms behind physiological bone healing. The exemplary depiction of 5 genes demonstrates the great complexity of metabolic processes during early bone healing. Here, BMP-2 signaling pathways and local hypoxia play decisive roles in bone formation.
AB - PURPOSE: Although bone tissue engineering techniques have become more and more sophisticated than in the past, natural bone healing mechanisms have not been sufficiently considered for further improvement of these techniques so far. We used an established animal model with transcriptome analysis to generate an unbiased picture of early bone healing to support tissue engineering concepts.MATERIAL AND METHODS: In 30 Wistar rats, a 3-mm bone defect was created in the mandibular angle. Tissue was sampled at 5, 10, and 15 days, and the former defect area was excised to undergo transcriptome analysis after RNA extraction. Five differentially expressed genes were further evaluated with reverse transcription-polymerase chain reaction (rt-PCR).RESULTS: Transcriptome analysis revealed 2467 significantly over- and under-expressed transcripts after 5 days and 2265 after 15 days of bone healing, respectively. Validation via rt-PCR confirmed overexpression of osteoactivin, angiopoietin-like factor-4, and metallomatrix proteinase-9 and underexpression of mastcellprotease-10 and proteoglycane-2 in comparison to values in the control group.CONCLUSION: This systematic genome-wide transcriptome analysis helps to decipher the physiological mechanisms behind physiological bone healing. The exemplary depiction of 5 genes demonstrates the great complexity of metabolic processes during early bone healing. Here, BMP-2 signaling pathways and local hypoxia play decisive roles in bone formation.
U2 - 10.1016/j.jcms.2015.06.015
DO - 10.1016/j.jcms.2015.06.015
M3 - SCORING: Journal article
C2 - 26189147
VL - 43
SP - 1452
EP - 1460
JO - J CRANIO MAXILL SURG
JF - J CRANIO MAXILL SURG
SN - 1010-5182
IS - 8
ER -