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Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice

Standard

Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. / Kaczmarczyk, Lech; Bansal, Vikas; Rajput, Ashish; Rahman, Raza-Ur; Krzyżak, Wiesław; Degen, Joachim; Poll, Stefanie; Fuhrmann, Martin; Bonn, Stefan; Jackson, Walker Scot.

in: PLOS BIOL, Jahrgang 17, Nr. 8, 08.2019, S. e3000374.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kaczmarczyk, L, Bansal, V, Rajput, A, Rahman, R-U, Krzyżak, W, Degen, J, Poll, S, Fuhrmann, M, Bonn, S & Jackson, WS 2019, 'Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice', PLOS BIOL, Jg. 17, Nr. 8, S. e3000374. https://doi.org/10.1371/journal.pbio.3000374

APA

Kaczmarczyk, L., Bansal, V., Rajput, A., Rahman, R-U., Krzyżak, W., Degen, J., Poll, S., Fuhrmann, M., Bonn, S., & Jackson, W. S. (2019). Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. PLOS BIOL, 17(8), e3000374. https://doi.org/10.1371/journal.pbio.3000374

Vancouver

Kaczmarczyk L, Bansal V, Rajput A, Rahman R-U, Krzyżak W, Degen J et al. Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. PLOS BIOL. 2019 Aug;17(8):e3000374. https://doi.org/10.1371/journal.pbio.3000374

Bibtex

@article{ac3ec71de0fb456cb6e9c1971a09ecab,
title = "Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice",
abstract = "A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.",
author = "Lech Kaczmarczyk and Vikas Bansal and Ashish Rajput and Raza-Ur Rahman and Wies{\l}aw Krzy{\.z}ak and Joachim Degen and Stefanie Poll and Martin Fuhrmann and Stefan Bonn and Jackson, {Walker Scot}",
year = "2019",
month = aug,
doi = "10.1371/journal.pbio.3000374",
language = "English",
volume = "17",
pages = "e3000374",
journal = "PLOS BIOL",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice

AU - Kaczmarczyk, Lech

AU - Bansal, Vikas

AU - Rajput, Ashish

AU - Rahman, Raza-Ur

AU - Krzyżak, Wiesław

AU - Degen, Joachim

AU - Poll, Stefanie

AU - Fuhrmann, Martin

AU - Bonn, Stefan

AU - Jackson, Walker Scot

PY - 2019/8

Y1 - 2019/8

N2 - A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.

AB - A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.

U2 - 10.1371/journal.pbio.3000374

DO - 10.1371/journal.pbio.3000374

M3 - SCORING: Journal article

C2 - 31393866

VL - 17

SP - e3000374

JO - PLOS BIOL

JF - PLOS BIOL

SN - 1544-9173

IS - 8

ER -