Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation.
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Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation. / Chua, John Jia En; Schob, Claudia; Rehbein, Monika; Gkogkas, Christos G; Richter, Dietmar; Kindler, Stefan.
in: SCI REP-UK, Jahrgang 2, 2012, S. 484.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation.
AU - Chua, John Jia En
AU - Schob, Claudia
AU - Rehbein, Monika
AU - Gkogkas, Christos G
AU - Richter, Dietmar
AU - Kindler, Stefan
PY - 2012
Y1 - 2012
N2 - In mammalian neurons, targeting and translation of specific mRNAs in dendrites contribute to synaptic plasticity. After nuclear export, mRNAs designated for dendritic transport are generally assumed to be translationally dormant and activity of individual synapses may locally trigger their extrasomatic translation. We show that the long, GC-rich 5'-untranslated region of dendritic SAPAP3 mRNA restricts translation initiation via a mechanism that involves an upstream open reading frame (uORF). In addition, the uORF enables the use of an alternative translation start site, permitting synthesis of two SAPAP3 isoforms from a single mRNA. While both isoforms progressively accumulate at postsynaptic densities during early rat brain development, their levels relative to each other vary in different adult rat brain areas. Thus, alternative translation initiation events appear to regulate relative expression of distinct SAPAP3 isoforms in different brain regions, which may function to influence synaptic plasticity.
AB - In mammalian neurons, targeting and translation of specific mRNAs in dendrites contribute to synaptic plasticity. After nuclear export, mRNAs designated for dendritic transport are generally assumed to be translationally dormant and activity of individual synapses may locally trigger their extrasomatic translation. We show that the long, GC-rich 5'-untranslated region of dendritic SAPAP3 mRNA restricts translation initiation via a mechanism that involves an upstream open reading frame (uORF). In addition, the uORF enables the use of an alternative translation start site, permitting synthesis of two SAPAP3 isoforms from a single mRNA. While both isoforms progressively accumulate at postsynaptic densities during early rat brain development, their levels relative to each other vary in different adult rat brain areas. Thus, alternative translation initiation events appear to regulate relative expression of distinct SAPAP3 isoforms in different brain regions, which may function to influence synaptic plasticity.
KW - Animals
KW - Humans
KW - Gene Expression Regulation
KW - Mice
KW - Rats
KW - Cell Line, Tumor
KW - Molecular Sequence Data
KW - Base Sequence
KW - Sequence Alignment
KW - Open Reading Frames
KW - Protein Biosynthesis
KW - Brain/metabolism
KW - 5' Untranslated Regions
KW - Codon, Initiator
KW - Nerve Tissue Proteins/biosynthesis/genetics
KW - Neuronal Plasticity/genetics
KW - Peptide Chain Initiation, Translational
KW - Protein Isoforms/biosynthesis/genetics
KW - RNA Isoforms
KW - Rodentia/genetics
KW - Animals
KW - Humans
KW - Gene Expression Regulation
KW - Mice
KW - Rats
KW - Cell Line, Tumor
KW - Molecular Sequence Data
KW - Base Sequence
KW - Sequence Alignment
KW - Open Reading Frames
KW - Protein Biosynthesis
KW - Brain/metabolism
KW - 5' Untranslated Regions
KW - Codon, Initiator
KW - Nerve Tissue Proteins/biosynthesis/genetics
KW - Neuronal Plasticity/genetics
KW - Peptide Chain Initiation, Translational
KW - Protein Isoforms/biosynthesis/genetics
KW - RNA Isoforms
KW - Rodentia/genetics
U2 - 10.1038/srep00484
DO - 10.1038/srep00484
M3 - SCORING: Journal article
VL - 2
SP - 484
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
ER -
