PortalPublikationenSurgical procedures and postsurgical tissue processing signi...

Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue

Standard

Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue. / David, Kerstin A; Unger, Florian T; Uhlig, Philipp; Juhl, Hartmut; Moore, Helen M; Compton, Carolyn; Nashan, Björn; Dörner, Arnulf; de Weerth, Andreas; Zornig, Carsten.

in: ONCOTARGET, Jahrgang 5, Nr. 22, 2014, S. 11017-28.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

David, KA, Unger, FT, Uhlig, P, Juhl, H, Moore, HM, Compton, C, Nashan, B, Dörner, A, de Weerth, A & Zornig, C 2014, 'Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue', ONCOTARGET, Jg. 5, Nr. 22, S. 11017-28.

APA

David, K. A., Unger, F. T., Uhlig, P., Juhl, H., Moore, H. M., Compton, C., Nashan, B., Dörner, A., de Weerth, A., & Zornig, C. (2014). Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue. ONCOTARGET, 5(22), 11017-28.

Vancouver

David KA, Unger FT, Uhlig P, Juhl H, Moore HM, Compton C et al. Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue. ONCOTARGET. 2014;5(22):11017-28.

Bibtex

@article{a8a8e2f8422f4745ba787c8f4d148fdb,
title = "Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue",
abstract = "An understanding of tissue data variability in relation to processing techniques during and postsurgery would be desirable when testing surgical specimens for clinical diagnostics, drug development, or identification of predictive biomarkers. Specimens of normal and colorectal cancer (CRC) tissues removed during colon and liver resection surgery were obtained at the beginning of surgery and postsurgically, tissue was fixed at 10, 20, and 45 minutes. Specimens were analyzed from 50 patients with primary CRC and 43 with intrahepatic metastasis of CRC using a whole genome gene expression array. Additionally, we focused on the epidermal growth factor receptor pathway and quantified proteins and their phosphorylation status in relation to tissue processing timepoints. Gene and protein expression data obtained from colorectal and liver specimens were influenced by tissue handling during surgery and by postsurgical processing time. To obtain reliable expression data, tissue processing for research and diagnostic purposes needs to be highly standardized.",
author = "David, {Kerstin A} and Unger, {Florian T} and Philipp Uhlig and Hartmut Juhl and Moore, {Helen M} and Carolyn Compton and Bj{\"o}rn Nashan and Arnulf D{\"o}rner and {de Weerth}, Andreas and Carsten Zornig",
year = "2014",
language = "English",
volume = "5",
pages = "11017--28",
journal = "ONCOTARGET",
issn = "1949-2553",
publisher = "IMPACT JOURNALS LLC",
number = "22",

}

RIS

TY - JOUR

T1 - Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue

AU - David, Kerstin A

AU - Unger, Florian T

AU - Uhlig, Philipp

AU - Juhl, Hartmut

AU - Moore, Helen M

AU - Compton, Carolyn

AU - Nashan, Björn

AU - Dörner, Arnulf

AU - de Weerth, Andreas

AU - Zornig, Carsten

PY - 2014

Y1 - 2014

N2 - An understanding of tissue data variability in relation to processing techniques during and postsurgery would be desirable when testing surgical specimens for clinical diagnostics, drug development, or identification of predictive biomarkers. Specimens of normal and colorectal cancer (CRC) tissues removed during colon and liver resection surgery were obtained at the beginning of surgery and postsurgically, tissue was fixed at 10, 20, and 45 minutes. Specimens were analyzed from 50 patients with primary CRC and 43 with intrahepatic metastasis of CRC using a whole genome gene expression array. Additionally, we focused on the epidermal growth factor receptor pathway and quantified proteins and their phosphorylation status in relation to tissue processing timepoints. Gene and protein expression data obtained from colorectal and liver specimens were influenced by tissue handling during surgery and by postsurgical processing time. To obtain reliable expression data, tissue processing for research and diagnostic purposes needs to be highly standardized.

AB - An understanding of tissue data variability in relation to processing techniques during and postsurgery would be desirable when testing surgical specimens for clinical diagnostics, drug development, or identification of predictive biomarkers. Specimens of normal and colorectal cancer (CRC) tissues removed during colon and liver resection surgery were obtained at the beginning of surgery and postsurgically, tissue was fixed at 10, 20, and 45 minutes. Specimens were analyzed from 50 patients with primary CRC and 43 with intrahepatic metastasis of CRC using a whole genome gene expression array. Additionally, we focused on the epidermal growth factor receptor pathway and quantified proteins and their phosphorylation status in relation to tissue processing timepoints. Gene and protein expression data obtained from colorectal and liver specimens were influenced by tissue handling during surgery and by postsurgical processing time. To obtain reliable expression data, tissue processing for research and diagnostic purposes needs to be highly standardized.

M3 - SCORING: Journal article

C2 - 25526028

VL - 5

SP - 11017

EP - 11028

JO - ONCOTARGET

JF - ONCOTARGET

SN - 1949-2553

IS - 22

ER -