Substantial in vivo proliferation of CD4(+) and CD8(+) T lymphocytes during secondary Listeria monocytogenes infection

TY - JOUR

T1 - Substantial in vivo proliferation of CD4(+) and CD8(+) T lymphocytes during secondary Listeria monocytogenes infection

AU - Mittrücker, H W

AU - Köhler, A

AU - Kaufmann, S H

PY - 2000/4/1

Y1 - 2000/4/1

N2 - In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4(+) and CD8(+) T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4(+) and CD8(+) T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4(+) T cells responded to heat-killed listeriae with the production of IFN-gamma. Thus, our results reveal that in addition to the large CD8(+) T cell population an almost equally large population of Listeria-reactive CD4(+) T cells is generated in response to L. monocytogenes infection.

AB - In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4(+) and CD8(+) T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4(+) and CD8(+) T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4(+) T cells responded to heat-killed listeriae with the production of IFN-gamma. Thus, our results reveal that in addition to the large CD8(+) T cell population an almost equally large population of Listeria-reactive CD4(+) T cells is generated in response to L. monocytogenes infection.

KW - Adoptive Transfer

KW - Animals

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Cell Division

KW - Cells, Cultured

KW - Epitopes, T-Lymphocyte

KW - Female

KW - Flow Cytometry

KW - Hot Temperature

KW - Interferon-gamma

KW - Kinetics

KW - Listeria monocytogenes

KW - Listeriosis

KW - Lymphocyte Activation

KW - Lymphocyte Count

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Spleen

U2 - 10.1002/(SICI)1521-4141(200004)30:4<1053::AID-IMMU1053>3.0.CO;2-N

DO - 10.1002/(SICI)1521-4141(200004)30:4<1053::AID-IMMU1053>3.0.CO;2-N

M3 - SCORING: Journal article

C2 - 10760793

VL - 30

SP - 1053

EP - 1059

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 4

ER -