Substantial differences in specificity of HIV-specific cytotoxic T cells in acute and chronic HIV infection
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Substantial differences in specificity of HIV-specific cytotoxic T cells in acute and chronic HIV infection. / Goulder, P J; Altfeld, M A; Rosenberg, E S; Nguyen, T; Tang, Y; Eldridge, R L; Addo, M M; He, S; Mukherjee, J S; Phillips, M N; Bunce, M; Kalams, S A; Sekaly, R P; Walker, B D; Brander, C.
in: J EXP MED, Jahrgang 193, Nr. 2, 15.01.2001, S. 181-94.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Substantial differences in specificity of HIV-specific cytotoxic T cells in acute and chronic HIV infection
AU - Goulder, P J
AU - Altfeld, M A
AU - Rosenberg, E S
AU - Nguyen, T
AU - Tang, Y
AU - Eldridge, R L
AU - Addo, M M
AU - He, S
AU - Mukherjee, J S
AU - Phillips, M N
AU - Bunce, M
AU - Kalams, S A
AU - Sekaly, R P
AU - Walker, B D
AU - Brander, C
PY - 2001/1/15
Y1 - 2001/1/15
N2 - Cytotoxic T lymphocytes (CTLs) play a vital part in controlling viral replication during human viral infections. Most studies in human infections have focused on CTL specificities in chronic infection and few data exist regarding the specificity of the initial CTL response induced in acute infection. In this study, HIV-1 infection in persons expressing human histocompatibility leukocyte antigen (HLA)-A*0201 was used as a means of addressing this issue. In chronic infection, the dominant HLA-A*0201-restricted CTL response is directed towards the epitope SLYNTVATL ("SL9") in p17 Gag (residues 77-85). This epitope is targeted by 75% of HLA-A*0201-positive adults, and the magnitude of this A*0201-SL9 response shows a strong negative association with viral load in progressive infection. Despite using the highly sensitive peptide-major histocompatibility complex tetramer and intracellular cytokine assays, responses to the SL9 epitope were not detectable in any of 11 HLA-A*0201-positive subjects with acute HIV-1 infection (P = 2 x 10(-6)), even when assays were repeated using the SL9 peptide variant that was encoded by their autologous virus. In contrast, multiple responses (median 3) to other epitopes were evident in 7 of the 11 A*0201-positive subjects. Longitudinal study of two subjects confirmed that the A*0201-SL9 response emerged later than other CTL responses, and after viral set point had been reached. Together, these data show that the CTL responses that are present and that even may dominate in chronic infection may differ substantially from those that constitute the initial antiviral CTL response. This finding is an important consideration in vaccine design and in the evaluation of vaccine candidates.
AB - Cytotoxic T lymphocytes (CTLs) play a vital part in controlling viral replication during human viral infections. Most studies in human infections have focused on CTL specificities in chronic infection and few data exist regarding the specificity of the initial CTL response induced in acute infection. In this study, HIV-1 infection in persons expressing human histocompatibility leukocyte antigen (HLA)-A*0201 was used as a means of addressing this issue. In chronic infection, the dominant HLA-A*0201-restricted CTL response is directed towards the epitope SLYNTVATL ("SL9") in p17 Gag (residues 77-85). This epitope is targeted by 75% of HLA-A*0201-positive adults, and the magnitude of this A*0201-SL9 response shows a strong negative association with viral load in progressive infection. Despite using the highly sensitive peptide-major histocompatibility complex tetramer and intracellular cytokine assays, responses to the SL9 epitope were not detectable in any of 11 HLA-A*0201-positive subjects with acute HIV-1 infection (P = 2 x 10(-6)), even when assays were repeated using the SL9 peptide variant that was encoded by their autologous virus. In contrast, multiple responses (median 3) to other epitopes were evident in 7 of the 11 A*0201-positive subjects. Longitudinal study of two subjects confirmed that the A*0201-SL9 response emerged later than other CTL responses, and after viral set point had been reached. Together, these data show that the CTL responses that are present and that even may dominate in chronic infection may differ substantially from those that constitute the initial antiviral CTL response. This finding is an important consideration in vaccine design and in the evaluation of vaccine candidates.
KW - Acute Disease
KW - Adult
KW - Amino Acid Sequence
KW - Chronic Disease
KW - Epitopes/genetics
KW - Female
KW - Gene Products, gag/genetics
KW - Genetic Variation
KW - HIV Antigens/genetics
KW - HIV Infections/immunology
KW - HIV-1/genetics
KW - HLA-A2 Antigen
KW - Humans
KW - Male
KW - Middle Aged
KW - T-Lymphocytes, Cytotoxic/immunology
KW - Time Factors
KW - Viral Proteins
KW - gag Gene Products, Human Immunodeficiency Virus
U2 - 10.1084/jem.193.2.181
DO - 10.1084/jem.193.2.181
M3 - SCORING: Journal article
C2 - 11148222
VL - 193
SP - 181
EP - 194
JO - J EXP MED
JF - J EXP MED
SN - 0022-1007
IS - 2
ER -