Subclones of C6 rat glioma cells differing in intermediate filament protein expression

K Röser; W Bohn; G Giese; K Mannweiler

Subclones of C6 rat glioma cells differing in intermediate filament protein expression

Standard

Subclones of C6 rat glioma cells differing in intermediate filament protein expression. / Röser, K; Bohn, W; Giese, G; Mannweiler, K.

in: EXP CELL RES, Jahrgang 197, Nr. 2, 12.1991, S. 200-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Röser, K, Bohn, W, Giese, G & Mannweiler, K 1991, 'Subclones of C6 rat glioma cells differing in intermediate filament protein expression', EXP CELL RES, Jg. 197, Nr. 2, S. 200-6.

APA

Röser, K., Bohn, W., Giese, G., & Mannweiler, K. (1991). Subclones of C6 rat glioma cells differing in intermediate filament protein expression. EXP CELL RES, 197(2), 200-6.

Vancouver

Röser K, Bohn W, Giese G, Mannweiler K. Subclones of C6 rat glioma cells differing in intermediate filament protein expression. EXP CELL RES. 1991 Dez;197(2):200-6.

Bibtex

@article{4b509f4954144c01923ea0a401d1bef8,

title = "Subclones of C6 rat glioma cells differing in intermediate filament protein expression",

abstract = "The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.",

keywords = "Animals, Blotting, Northern, Blotting, Western, Cell Division, Clone Cells, Cytoskeleton, Gene Expression, Glioma, Intermediate Filament Proteins, Kinetics, Poly A, Protein Biosynthesis, RNA, RNA, Messenger, RNA, Neoplasm, Rats, Vimentin, Journal Article, Research Support, Non-U.S. Gov't",

author = "K R{\"o}ser and W Bohn and G Giese and K Mannweiler",

year = "1991",

month = dec,

language = "English",

volume = "197",

pages = "200--6",

journal = "EXP CELL RES",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "2",

}

RIS

TY - JOUR

T1 - Subclones of C6 rat glioma cells differing in intermediate filament protein expression

AU - Röser, K

AU - Bohn, W

AU - Giese, G

AU - Mannweiler, K

PY - 1991/12

Y1 - 1991/12

N2 - The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.

AB - The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.

KW - Animals

KW - Blotting, Northern

KW - Blotting, Western

KW - Cell Division

KW - Clone Cells

KW - Cytoskeleton

KW - Gene Expression

KW - Glioma

KW - Intermediate Filament Proteins

KW - Kinetics

KW - Poly A

KW - Protein Biosynthesis

KW - RNA

KW - RNA, Messenger

KW - RNA, Neoplasm

KW - Rats

KW - Vimentin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 1720390

VL - 197

SP - 200

EP - 206

JO - EXP CELL RES

JF - EXP CELL RES

SN - 0014-4827

IS - 2

ER -