Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor.
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Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor. / García-Fernández, Rossana; Pons, Tirso; Perbandt, Markus; Valiente, Pedro A; Talavera, Ariel; González-González, Yamile; Rehders, Dirk; Chávez, María A; Betzel, Christian; Redecke, Lars.
in: J STRUCT BIOL, Jahrgang 180, Nr. 2, 2, 2012, S. 271-279.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor.
AU - García-Fernández, Rossana
AU - Pons, Tirso
AU - Perbandt, Markus
AU - Valiente, Pedro A
AU - Talavera, Ariel
AU - González-González, Yamile
AU - Rehders, Dirk
AU - Chávez, María A
AU - Betzel, Christian
AU - Redecke, Lars
PY - 2012
Y1 - 2012
N2 - Proteins isolated from marine invertebrates are frequently characterized by exceptional structural and functional properties. ShPI-1, a BPTI Kunitz-type inhibitor from the Caribbean Sea anemone Stichodactyla helianthus, displays activity not only against serine-, but also against cysteine-, and aspartate proteases. As an initial step to evaluate the molecular basis of its activities, we describe the crystallographic structure of ShPI-1 in complex with the serine protease bovine pancreatic trypsin at 1.7Å resolution. The overall structure and the important enzyme-inhibitor interactions of this first invertebrate BPTI-like Kunitz-type inhibitor:trypsin complex remained largely conserved compared to mammalian BPTI-Kunitz inhibitor complexes. However, a prominent stabilizing role within the interface was attributed to arginine at position P3. Binding free-energy calculations indicated a 10-fold decrease for the inhibitor affinity against trypsin, if the P3 residue of ShPI-1 is mutated to alanine. Together with the increased role of Arg(11) at P3 position, slightly reduced interactions at the prime side (Pn') of the primary binding loop and at the secondary binding loop of ShPI-1 were detected. In addition, the structure provides important information for site directed mutagenesis to further optimize the activity of rShPI-1A for biotechnological applications.
AB - Proteins isolated from marine invertebrates are frequently characterized by exceptional structural and functional properties. ShPI-1, a BPTI Kunitz-type inhibitor from the Caribbean Sea anemone Stichodactyla helianthus, displays activity not only against serine-, but also against cysteine-, and aspartate proteases. As an initial step to evaluate the molecular basis of its activities, we describe the crystallographic structure of ShPI-1 in complex with the serine protease bovine pancreatic trypsin at 1.7Å resolution. The overall structure and the important enzyme-inhibitor interactions of this first invertebrate BPTI-like Kunitz-type inhibitor:trypsin complex remained largely conserved compared to mammalian BPTI-Kunitz inhibitor complexes. However, a prominent stabilizing role within the interface was attributed to arginine at position P3. Binding free-energy calculations indicated a 10-fold decrease for the inhibitor affinity against trypsin, if the P3 residue of ShPI-1 is mutated to alanine. Together with the increased role of Arg(11) at P3 position, slightly reduced interactions at the prime side (Pn') of the primary binding loop and at the secondary binding loop of ShPI-1 were detected. In addition, the structure provides important information for site directed mutagenesis to further optimize the activity of rShPI-1A for biotechnological applications.
KW - Animals
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Cattle
KW - Mutagenesis, Site-Directed
KW - Crystallography, X-Ray
KW - Sea Anemones
KW - Serine Endopeptidases/chemistry/metabolism
KW - Serine Proteinase Inhibitors/chemistry/pharmacology
KW - Trypsin/metabolism
KW - Animals
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Cattle
KW - Mutagenesis, Site-Directed
KW - Crystallography, X-Ray
KW - Sea Anemones
KW - Serine Endopeptidases/chemistry/metabolism
KW - Serine Proteinase Inhibitors/chemistry/pharmacology
KW - Trypsin/metabolism
M3 - SCORING: Journal article
VL - 180
SP - 271
EP - 279
JO - J STRUCT BIOL
JF - J STRUCT BIOL
SN - 1047-8477
IS - 2
M1 - 2
ER -