Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate
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Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate. / Yuan, Biao; Portaliou, Athina G; Parakra, Rinky; Smit, Jochem H; Wald, Jiri; Li, Yichen; Srinivasu, Bindu; Loos, Maria S; Dhupar, Harveer Singh; Fahrenkamp, Dirk; Kalodimos, Charalampos G; Duong van Hoa, Franck; Cordes, Thorben; Karamanou, Spyridoula; Marlovits, Thomas C; Economou, Anastassios.
in: J MOL BIOL, Jahrgang 433, Nr. 21, 15.10.2021, S. 167188.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate
AU - Yuan, Biao
AU - Portaliou, Athina G
AU - Parakra, Rinky
AU - Smit, Jochem H
AU - Wald, Jiri
AU - Li, Yichen
AU - Srinivasu, Bindu
AU - Loos, Maria S
AU - Dhupar, Harveer Singh
AU - Fahrenkamp, Dirk
AU - Kalodimos, Charalampos G
AU - Duong van Hoa, Franck
AU - Cordes, Thorben
AU - Karamanou, Spyridoula
AU - Marlovits, Thomas C
AU - Economou, Anastassios
N1 - Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.
PY - 2021/10/15
Y1 - 2021/10/15
N2 - Type III protein secretion is widespread in Gram-negative pathogens. It comprises the injectisome with a surface-exposed needle and an inner membrane translocase. The translocase contains the SctRSTU export channel enveloped by the export gate subunit SctV that binds chaperone/exported clients and forms a putative ante-chamber. We probed the assembly, function, structure and dynamics of SctV from enteropathogenic E. coli (EPEC). In both EPEC and E. coli lab strains, SctV forms peripheral oligomeric clusters that are detergent-extracted as homo-nonamers. Membrane-embedded SctV9 is necessary and sufficient to act as a receptor for different chaperone/exported protein pairs with distinct C-domain binding sites that are essential for secretion. Negative staining electron microscopy revealed that peptidisc-reconstituted His-SctV9 forms a tripartite particle of ∼22 nm with a N-terminal domain connected by a short linker to a C-domain ring structure with a ∼5 nm-wide inner opening. The isolated C-domain ring was resolved with cryo-EM at 3.1 Å and structurally compared to other SctV homologues. Its four sub-domains undergo a three-stage "pinching" motion. Hydrogen-deuterium exchange mass spectrometry revealed this to involve dynamic and rigid hinges and a hyper-flexible sub-domain that flips out of the ring periphery and binds chaperones on and between adjacent protomers. These motions are coincident with local conformational changes at the pore surface and ring entry mouth that may also be modulated by the ATPase inner stalk. We propose that the intrinsic dynamics of the SctV protomer are modulated by chaperones and the ATPase and could affect allosterically the other subunits of the nonameric ring during secretion.
AB - Type III protein secretion is widespread in Gram-negative pathogens. It comprises the injectisome with a surface-exposed needle and an inner membrane translocase. The translocase contains the SctRSTU export channel enveloped by the export gate subunit SctV that binds chaperone/exported clients and forms a putative ante-chamber. We probed the assembly, function, structure and dynamics of SctV from enteropathogenic E. coli (EPEC). In both EPEC and E. coli lab strains, SctV forms peripheral oligomeric clusters that are detergent-extracted as homo-nonamers. Membrane-embedded SctV9 is necessary and sufficient to act as a receptor for different chaperone/exported protein pairs with distinct C-domain binding sites that are essential for secretion. Negative staining electron microscopy revealed that peptidisc-reconstituted His-SctV9 forms a tripartite particle of ∼22 nm with a N-terminal domain connected by a short linker to a C-domain ring structure with a ∼5 nm-wide inner opening. The isolated C-domain ring was resolved with cryo-EM at 3.1 Å and structurally compared to other SctV homologues. Its four sub-domains undergo a three-stage "pinching" motion. Hydrogen-deuterium exchange mass spectrometry revealed this to involve dynamic and rigid hinges and a hyper-flexible sub-domain that flips out of the ring periphery and binds chaperones on and between adjacent protomers. These motions are coincident with local conformational changes at the pore surface and ring entry mouth that may also be modulated by the ATPase inner stalk. We propose that the intrinsic dynamics of the SctV protomer are modulated by chaperones and the ATPase and could affect allosterically the other subunits of the nonameric ring during secretion.
U2 - 10.1016/j.jmb.2021.167188
DO - 10.1016/j.jmb.2021.167188
M3 - SCORING: Journal article
C2 - 34454944
VL - 433
SP - 167188
JO - J MOL BIOL
JF - J MOL BIOL
SN - 0022-2836
IS - 21
ER -