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Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.).

Standard

Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.). / Brecker, Lothar; Wicklein, Daniel; Moll, Hermann; Fuchs, Elmar C; Becker, Wolf-Meinhard; Petersen, Arnd.

in: CARBOHYD RES, Jahrgang 340, Nr. 4, 4, 2005, S. 657-663.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Brecker, L, Wicklein, D, Moll, H, Fuchs, EC, Becker, W-M & Petersen, A 2005, 'Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.).', CARBOHYD RES, Jg. 340, Nr. 4, 4, S. 657-663. <http://www.ncbi.nlm.nih.gov/pubmed/15721337?dopt=Citation>

APA

Brecker, L., Wicklein, D., Moll, H., Fuchs, E. C., Becker, W-M., & Petersen, A. (2005). Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.). CARBOHYD RES, 340(4), 657-663. [4]. http://www.ncbi.nlm.nih.gov/pubmed/15721337?dopt=Citation

Vancouver

Brecker L, Wicklein D, Moll H, Fuchs EC, Becker W-M, Petersen A. Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.). CARBOHYD RES. 2005;340(4):657-663. 4.

Bibtex

@article{30568c91051f4257ab36f7c0559aa579,
title = "Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.).",
abstract = "Extracts from pollen of timothy grass (Phleum pratense L.) contain up to 20% arabinogalactan proteins (AGPs). Separation of the AGP polysaccharide moieties by tryptic digestion, size exclusion chromatography (GPC), and reverse phase HPLC yielded arabinogalactan fractions AG-1 and AG-2 with molecular weights of approximately 15,000 and approximately 60,000Da, respectively. The backbones of both polysaccharides are composed of (1-->6)-linked beta-D-galactopyranosides with beta-D-GlcUAp or 4-O-Me-beta-D-GlcUAp at their terminal ends as revealed by chemical analysis, FT-IR, MALDI-MS, and NMR spectroscopy. AG-1 contains a small number of beta-l-Araf side chains while AG-2 possesses a variety of (1-->3)-linked units, which consist of beta-l-Araf-(1-->, alpha-l-Araf-(1-->3)-beta-l-Araf-(1-->, and alpha-l-Araf-(1-->5)-beta-l-Araf-(1--> as well as a small number of longer arabinogalactan side chains. In contrast to crude pollen extracts, the immunological properties of the arabinogalactan mixture reveal an IgG4 reactivity instead of IgE reactivity. Structural properties of timothy pollen arabinogalactan might thus influence the immune response.",
keywords = "Chromatography, High Pressure Liquid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Molecular Structure, Molecular Weight, Mass Spectrometry, Immunoglobulin G/immunology, Allergens/*immunology, Carbohydrate Sequence, Chromatography, Gel, Galactans/*chemistry/*immunology, Nuclear Magnetic Resonance, Biomolecular, Phleum/*immunology, Pollen/*immunology, Polysaccharides, Bacterial/chemistry/immunology, Spectroscopy, Fourier Transform Infrared, Chromatography, High Pressure Liquid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Molecular Structure, Molecular Weight, Mass Spectrometry, Immunoglobulin G/immunology, Allergens/*immunology, Carbohydrate Sequence, Chromatography, Gel, Galactans/*chemistry/*immunology, Nuclear Magnetic Resonance, Biomolecular, Phleum/*immunology, Pollen/*immunology, Polysaccharides, Bacterial/chemistry/immunology, Spectroscopy, Fourier Transform Infrared",
author = "Lothar Brecker and Daniel Wicklein and Hermann Moll and Fuchs, {Elmar C} and Wolf-Meinhard Becker and Arnd Petersen",
year = "2005",
language = "English",
volume = "340",
pages = "657--663",
journal = "CARBOHYD RES",
issn = "0008-6215",
publisher = "Elsevier BV",
number = "4",

}

RIS

TY - JOUR

T1 - Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.).

AU - Brecker, Lothar

AU - Wicklein, Daniel

AU - Moll, Hermann

AU - Fuchs, Elmar C

AU - Becker, Wolf-Meinhard

AU - Petersen, Arnd

PY - 2005

Y1 - 2005

N2 - Extracts from pollen of timothy grass (Phleum pratense L.) contain up to 20% arabinogalactan proteins (AGPs). Separation of the AGP polysaccharide moieties by tryptic digestion, size exclusion chromatography (GPC), and reverse phase HPLC yielded arabinogalactan fractions AG-1 and AG-2 with molecular weights of approximately 15,000 and approximately 60,000Da, respectively. The backbones of both polysaccharides are composed of (1-->6)-linked beta-D-galactopyranosides with beta-D-GlcUAp or 4-O-Me-beta-D-GlcUAp at their terminal ends as revealed by chemical analysis, FT-IR, MALDI-MS, and NMR spectroscopy. AG-1 contains a small number of beta-l-Araf side chains while AG-2 possesses a variety of (1-->3)-linked units, which consist of beta-l-Araf-(1-->, alpha-l-Araf-(1-->3)-beta-l-Araf-(1-->, and alpha-l-Araf-(1-->5)-beta-l-Araf-(1--> as well as a small number of longer arabinogalactan side chains. In contrast to crude pollen extracts, the immunological properties of the arabinogalactan mixture reveal an IgG4 reactivity instead of IgE reactivity. Structural properties of timothy pollen arabinogalactan might thus influence the immune response.

AB - Extracts from pollen of timothy grass (Phleum pratense L.) contain up to 20% arabinogalactan proteins (AGPs). Separation of the AGP polysaccharide moieties by tryptic digestion, size exclusion chromatography (GPC), and reverse phase HPLC yielded arabinogalactan fractions AG-1 and AG-2 with molecular weights of approximately 15,000 and approximately 60,000Da, respectively. The backbones of both polysaccharides are composed of (1-->6)-linked beta-D-galactopyranosides with beta-D-GlcUAp or 4-O-Me-beta-D-GlcUAp at their terminal ends as revealed by chemical analysis, FT-IR, MALDI-MS, and NMR spectroscopy. AG-1 contains a small number of beta-l-Araf side chains while AG-2 possesses a variety of (1-->3)-linked units, which consist of beta-l-Araf-(1-->, alpha-l-Araf-(1-->3)-beta-l-Araf-(1-->, and alpha-l-Araf-(1-->5)-beta-l-Araf-(1--> as well as a small number of longer arabinogalactan side chains. In contrast to crude pollen extracts, the immunological properties of the arabinogalactan mixture reveal an IgG4 reactivity instead of IgE reactivity. Structural properties of timothy pollen arabinogalactan might thus influence the immune response.

KW - Chromatography, High Pressure Liquid

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Molecular Structure

KW - Molecular Weight

KW - Mass Spectrometry

KW - Immunoglobulin G/immunology

KW - Allergens/immunology

KW - Carbohydrate Sequence

KW - Chromatography, Gel

KW - Galactans/chemistry/immunology

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Phleum/immunology

KW - Pollen/immunology

KW - Polysaccharides, Bacterial/chemistry/immunology

KW - Spectroscopy, Fourier Transform Infrared

KW - Chromatography, High Pressure Liquid

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Molecular Structure

KW - Molecular Weight

KW - Mass Spectrometry

KW - Immunoglobulin G/immunology

KW - Allergens/immunology

KW - Carbohydrate Sequence

KW - Chromatography, Gel

KW - Galactans/chemistry/immunology

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Phleum/immunology

KW - Pollen/immunology

KW - Polysaccharides, Bacterial/chemistry/immunology

KW - Spectroscopy, Fourier Transform Infrared

M3 - SCORING: Journal article

VL - 340

SP - 657

EP - 663

JO - CARBOHYD RES

JF - CARBOHYD RES

SN - 0008-6215

IS - 4

M1 - 4

ER -