Stem/progenitor cells in non-lactating versus lactating equine mammary gland.
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Stem/progenitor cells in non-lactating versus lactating equine mammary gland. / Spaas, Jan H; Chiers, Koen; Bussche van den, Hendrik; Burvenich, Christian; Walle, Van de; Gerlinde, R.
in: STEM CELLS DEV, Jahrgang 21, Nr. 16, 16, 2012, S. 3055-3067.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Stem/progenitor cells in non-lactating versus lactating equine mammary gland.
AU - Spaas, Jan H
AU - Chiers, Koen
AU - Bussche van den, Hendrik
AU - Burvenich, Christian
AU - Walle, Van de
AU - Gerlinde, R
PY - 2012
Y1 - 2012
N2 - The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts that (i) mammary stem/progenitor cells (MaSC) are proposed to be the driving forces behind mammary growth and function and (ii) variation exists between mammalian species with regard to physiological and pathological functioning of this organ, we believe that studying MaSC from different mammals is of great comparative interest. Over the years, important data has been gathered on MaSC of men and mice, although knowledge on MaSC in other mammals remains limited. Therefore, the aim of this work was to isolate and characterize MaSC from the mammary gland of horses. Hereby, our salient findings were that the isolated equine cells met the 2 in vitro hallmark properties of stem cells, namely the ability to self-renew and to differentiate into multiple cell lineages. Moreover, the cells were immunophenotyped using markers for CD29, CD44, CD49f, and Ki67. Finally, we propose the mammosphere assay as a valuable in vitro assay to study MaSC during different physiological phases since it was observed that equine lactating mammary gland contains significantly more mammosphere-initiating cells than the inactive, nonlactating gland (a reflection of MaSC self-renewal) and, moreover, that these spheres were significantly larger in size upon initial cultivation (a reflection of progenitor cell proliferation). Taken together, this study not only extends the current knowledge of mammary gland biology, but also benefits the comparative approach to study and compare MaSC in different mammalian species.
AB - The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts that (i) mammary stem/progenitor cells (MaSC) are proposed to be the driving forces behind mammary growth and function and (ii) variation exists between mammalian species with regard to physiological and pathological functioning of this organ, we believe that studying MaSC from different mammals is of great comparative interest. Over the years, important data has been gathered on MaSC of men and mice, although knowledge on MaSC in other mammals remains limited. Therefore, the aim of this work was to isolate and characterize MaSC from the mammary gland of horses. Hereby, our salient findings were that the isolated equine cells met the 2 in vitro hallmark properties of stem cells, namely the ability to self-renew and to differentiate into multiple cell lineages. Moreover, the cells were immunophenotyped using markers for CD29, CD44, CD49f, and Ki67. Finally, we propose the mammosphere assay as a valuable in vitro assay to study MaSC during different physiological phases since it was observed that equine lactating mammary gland contains significantly more mammosphere-initiating cells than the inactive, nonlactating gland (a reflection of MaSC self-renewal) and, moreover, that these spheres were significantly larger in size upon initial cultivation (a reflection of progenitor cell proliferation). Taken together, this study not only extends the current knowledge of mammary gland biology, but also benefits the comparative approach to study and compare MaSC in different mammalian species.
KW - Animals
KW - Humans
KW - Female
KW - Cells, Cultured
KW - Mice
KW - Immunophenotyping
KW - Cell Proliferation
KW - Cell Differentiation
KW - Cell Cycle
KW - Biological Markers/metabolism
KW - Epithelial Cells/cytology/metabolism
KW - Adipogenesis
KW - Staining and Labeling
KW - Clone Cells
KW - Adipocytes/cytology/metabolism
KW - Colony-Forming Units Assay
KW - Horses/physiology
KW - Lactation/physiology
KW - Mammary Glands, Animal/cytology/physiology
KW - Spheroids, Cellular/cytology/metabolism
KW - Stem Cells/cytology
KW - Animals
KW - Humans
KW - Female
KW - Cells, Cultured
KW - Mice
KW - Immunophenotyping
KW - Cell Proliferation
KW - Cell Differentiation
KW - Cell Cycle
KW - Biological Markers/metabolism
KW - Epithelial Cells/cytology/metabolism
KW - Adipogenesis
KW - Staining and Labeling
KW - Clone Cells
KW - Adipocytes/cytology/metabolism
KW - Colony-Forming Units Assay
KW - Horses/physiology
KW - Lactation/physiology
KW - Mammary Glands, Animal/cytology/physiology
KW - Spheroids, Cellular/cytology/metabolism
KW - Stem Cells/cytology
M3 - SCORING: Journal article
VL - 21
SP - 3055
EP - 3067
JO - STEM CELLS DEV
JF - STEM CELLS DEV
SN - 1547-3287
IS - 16
M1 - 16
ER -
