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Stem cell transplantation: the lung barrier

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Stem cell transplantation: the lung barrier. / Schrepfer, S; Deuse, T; Reichenspurner, H; Fischbein, M P; Robbins, R C; Pelletier, M P.

in: TRANSPL P, Jahrgang 39, Nr. 2, 03.2007, S. 573-576.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schrepfer, S, Deuse, T, Reichenspurner, H, Fischbein, MP, Robbins, RC & Pelletier, MP 2007, 'Stem cell transplantation: the lung barrier', TRANSPL P, Jg. 39, Nr. 2, S. 573-576. https://doi.org/10.1016/j.transproceed.2006.12.019

APA

Schrepfer, S., Deuse, T., Reichenspurner, H., Fischbein, M. P., Robbins, R. C., & Pelletier, M. P. (2007). Stem cell transplantation: the lung barrier. TRANSPL P, 39(2), 573-576. https://doi.org/10.1016/j.transproceed.2006.12.019

Vancouver

Schrepfer S, Deuse T, Reichenspurner H, Fischbein MP, Robbins RC, Pelletier MP. Stem cell transplantation: the lung barrier. TRANSPL P. 2007 Mär;39(2):573-576. https://doi.org/10.1016/j.transproceed.2006.12.019

Bibtex

@article{dbe93bbe51d548b0aac0f39f2e291659,
title = "Stem cell transplantation: the lung barrier",
abstract = "BACKGROUND: Mesenchymal stem cells (MSCs) show differentiation capacity along mesenchymal lineages and have the potential to aid tissue regeneration. MSC transplantation strategies are therefore currently being assessed following injury to various organs. However, potential MSC migration to these organs after intravenous (IV) MSC injection continues to be impeded by cell trapping within the lung.METHODS: Mouse MSCs were isolated, purified, transfected with firefly luciferase, and labeled with CSFE. Their size was assessed in vitro. To estimate the diameter of mouse pulmonary capillaries, fluorescence-labeled microspheres of different sizes were injected with or without sodium nitroprusside (SN) pretreatment. The lungs were harvested after 30 seconds and mean numbers of trapped microspheres per high-power field (HPF) were calculated. After IV injection of MSC suspensions (with or without SN), their dynamic distribution was monitored by in vivo luciferine imaging as well as by histopathology.RESULTS: The diameter of suspended MSCs in vitro was 15 to 19 microm. Whereas nearly no 4-microm microspheres could be detected in lung sections, the numbers of trapped 10- and 15-microm microspheres could be significantly decreased by prior SN injection from 19.3 +/- 11.1 to 6.0 +/- 1.6 cells/HPF (P = .004) and from 34.9 +/- 11.9 to 25.6 +/- 8.1 cells/HPF (P = .028), respectively. Within seconds after MSC IV injection, the vast majority of cells was found in the lungs. However, cell trapping in the pulmonary microvasculature was significantly reduced by pre-treatment with SN.CONCLUSIONS: We demonstrate that cell trapping in lungs can be reduced with IV SN pretreatment, increasing MSC passage through the lung capillaries, and potentially facilitating cell access to injured organs.",
keywords = "Animals, Infusions, Intravenous, Luciferases/analysis, Lung/physiopathology, Mesenchymal Stem Cell Transplantation/adverse effects, Mice, Mice, Inbred BALB C, Recombinant Proteins/analysis, Transfection",
author = "S Schrepfer and T Deuse and H Reichenspurner and Fischbein, {M P} and Robbins, {R C} and Pelletier, {M P}",
year = "2007",
month = mar,
doi = "10.1016/j.transproceed.2006.12.019",
language = "English",
volume = "39",
pages = "573--576",
journal = "TRANSPL P",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "2",

}

RIS

TY - JOUR

T1 - Stem cell transplantation: the lung barrier

AU - Schrepfer, S

AU - Deuse, T

AU - Reichenspurner, H

AU - Fischbein, M P

AU - Robbins, R C

AU - Pelletier, M P

PY - 2007/3

Y1 - 2007/3

N2 - BACKGROUND: Mesenchymal stem cells (MSCs) show differentiation capacity along mesenchymal lineages and have the potential to aid tissue regeneration. MSC transplantation strategies are therefore currently being assessed following injury to various organs. However, potential MSC migration to these organs after intravenous (IV) MSC injection continues to be impeded by cell trapping within the lung.METHODS: Mouse MSCs were isolated, purified, transfected with firefly luciferase, and labeled with CSFE. Their size was assessed in vitro. To estimate the diameter of mouse pulmonary capillaries, fluorescence-labeled microspheres of different sizes were injected with or without sodium nitroprusside (SN) pretreatment. The lungs were harvested after 30 seconds and mean numbers of trapped microspheres per high-power field (HPF) were calculated. After IV injection of MSC suspensions (with or without SN), their dynamic distribution was monitored by in vivo luciferine imaging as well as by histopathology.RESULTS: The diameter of suspended MSCs in vitro was 15 to 19 microm. Whereas nearly no 4-microm microspheres could be detected in lung sections, the numbers of trapped 10- and 15-microm microspheres could be significantly decreased by prior SN injection from 19.3 +/- 11.1 to 6.0 +/- 1.6 cells/HPF (P = .004) and from 34.9 +/- 11.9 to 25.6 +/- 8.1 cells/HPF (P = .028), respectively. Within seconds after MSC IV injection, the vast majority of cells was found in the lungs. However, cell trapping in the pulmonary microvasculature was significantly reduced by pre-treatment with SN.CONCLUSIONS: We demonstrate that cell trapping in lungs can be reduced with IV SN pretreatment, increasing MSC passage through the lung capillaries, and potentially facilitating cell access to injured organs.

AB - BACKGROUND: Mesenchymal stem cells (MSCs) show differentiation capacity along mesenchymal lineages and have the potential to aid tissue regeneration. MSC transplantation strategies are therefore currently being assessed following injury to various organs. However, potential MSC migration to these organs after intravenous (IV) MSC injection continues to be impeded by cell trapping within the lung.METHODS: Mouse MSCs were isolated, purified, transfected with firefly luciferase, and labeled with CSFE. Their size was assessed in vitro. To estimate the diameter of mouse pulmonary capillaries, fluorescence-labeled microspheres of different sizes were injected with or without sodium nitroprusside (SN) pretreatment. The lungs were harvested after 30 seconds and mean numbers of trapped microspheres per high-power field (HPF) were calculated. After IV injection of MSC suspensions (with or without SN), their dynamic distribution was monitored by in vivo luciferine imaging as well as by histopathology.RESULTS: The diameter of suspended MSCs in vitro was 15 to 19 microm. Whereas nearly no 4-microm microspheres could be detected in lung sections, the numbers of trapped 10- and 15-microm microspheres could be significantly decreased by prior SN injection from 19.3 +/- 11.1 to 6.0 +/- 1.6 cells/HPF (P = .004) and from 34.9 +/- 11.9 to 25.6 +/- 8.1 cells/HPF (P = .028), respectively. Within seconds after MSC IV injection, the vast majority of cells was found in the lungs. However, cell trapping in the pulmonary microvasculature was significantly reduced by pre-treatment with SN.CONCLUSIONS: We demonstrate that cell trapping in lungs can be reduced with IV SN pretreatment, increasing MSC passage through the lung capillaries, and potentially facilitating cell access to injured organs.

KW - Animals

KW - Infusions, Intravenous

KW - Luciferases/analysis

KW - Lung/physiopathology

KW - Mesenchymal Stem Cell Transplantation/adverse effects

KW - Mice

KW - Mice, Inbred BALB C

KW - Recombinant Proteins/analysis

KW - Transfection

U2 - 10.1016/j.transproceed.2006.12.019

DO - 10.1016/j.transproceed.2006.12.019

M3 - SCORING: Journal article

C2 - 17362785

VL - 39

SP - 573

EP - 576

JO - TRANSPL P

JF - TRANSPL P

SN - 0041-1345

IS - 2

ER -