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Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse

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Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse. / Sibbe, Mirjam; Kuner, Emanuel; Althof, Daniel; Frotscher, Michael.

in: PLOS ONE, Jahrgang 10, Nr. 3, 2015, S. e0119643.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Sibbe, M, Kuner, E, Althof, D & Frotscher, M 2015, 'Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse', PLOS ONE, Jg. 10, Nr. 3, S. e0119643. https://doi.org/10.1371/journal.pone.0119643

APA

Sibbe, M., Kuner, E., Althof, D., & Frotscher, M. (2015). Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse. PLOS ONE, 10(3), e0119643. https://doi.org/10.1371/journal.pone.0119643

Vancouver

Sibbe M, Kuner E, Althof D, Frotscher M. Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse. PLOS ONE. 2015;10(3):e0119643. https://doi.org/10.1371/journal.pone.0119643

Bibtex

@article{be3956c34964450c9577e13c34c540d8,
title = "Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse",
abstract = "Adult hippocampal neurogenesis has been implicated in hippocampus-dependent learning and memory. Furthermore, the decline of neurogenesis accompanying aging could be involved in age-related cognitive deficits. It is believed that the neural stem cell niche comprises a specialized microenvironment regulating stem cell activation and maintenance. However, little is known about the significance of the extracellular matrix in controlling adult stem cells. Reelin is a large glycoprotein of the extracelluar matrix known to be of crucial importance for neuronal migration. Here, we examined the local interrelation between Reelin expressing interneurons and putative hippocampal stem cells and investigated the effects of Reelin deficiency on stem cell and progenitor cell proliferation. Reelin-positive cells are found in close vicinity to putative stem cell processes, which would allow for stem cell regulation by Reelin. We investigated the proliferation of stem cells in the Reelin-deficient reeler hippocampus by Ki67 labeling and found a strong reduction of mitotic cells. A detailed analysis of dividing Type 1, type 2 and type 3 cells indicated that once a stem cell is recruited for proliferation, the progression to the next progenitor stage as well as the number of mitotic cycles is not altered in reeler. Our data point to a role for Reelin in either regulating stem cell quiescence or maintenance.",
author = "Mirjam Sibbe and Emanuel Kuner and Daniel Althof and Michael Frotscher",
year = "2015",
doi = "10.1371/journal.pone.0119643",
language = "English",
volume = "10",
pages = "e0119643",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Stem- and progenitor cell proliferation in the dentate gyrus of the reeler mouse

AU - Sibbe, Mirjam

AU - Kuner, Emanuel

AU - Althof, Daniel

AU - Frotscher, Michael

PY - 2015

Y1 - 2015

N2 - Adult hippocampal neurogenesis has been implicated in hippocampus-dependent learning and memory. Furthermore, the decline of neurogenesis accompanying aging could be involved in age-related cognitive deficits. It is believed that the neural stem cell niche comprises a specialized microenvironment regulating stem cell activation and maintenance. However, little is known about the significance of the extracellular matrix in controlling adult stem cells. Reelin is a large glycoprotein of the extracelluar matrix known to be of crucial importance for neuronal migration. Here, we examined the local interrelation between Reelin expressing interneurons and putative hippocampal stem cells and investigated the effects of Reelin deficiency on stem cell and progenitor cell proliferation. Reelin-positive cells are found in close vicinity to putative stem cell processes, which would allow for stem cell regulation by Reelin. We investigated the proliferation of stem cells in the Reelin-deficient reeler hippocampus by Ki67 labeling and found a strong reduction of mitotic cells. A detailed analysis of dividing Type 1, type 2 and type 3 cells indicated that once a stem cell is recruited for proliferation, the progression to the next progenitor stage as well as the number of mitotic cycles is not altered in reeler. Our data point to a role for Reelin in either regulating stem cell quiescence or maintenance.

AB - Adult hippocampal neurogenesis has been implicated in hippocampus-dependent learning and memory. Furthermore, the decline of neurogenesis accompanying aging could be involved in age-related cognitive deficits. It is believed that the neural stem cell niche comprises a specialized microenvironment regulating stem cell activation and maintenance. However, little is known about the significance of the extracellular matrix in controlling adult stem cells. Reelin is a large glycoprotein of the extracelluar matrix known to be of crucial importance for neuronal migration. Here, we examined the local interrelation between Reelin expressing interneurons and putative hippocampal stem cells and investigated the effects of Reelin deficiency on stem cell and progenitor cell proliferation. Reelin-positive cells are found in close vicinity to putative stem cell processes, which would allow for stem cell regulation by Reelin. We investigated the proliferation of stem cells in the Reelin-deficient reeler hippocampus by Ki67 labeling and found a strong reduction of mitotic cells. A detailed analysis of dividing Type 1, type 2 and type 3 cells indicated that once a stem cell is recruited for proliferation, the progression to the next progenitor stage as well as the number of mitotic cycles is not altered in reeler. Our data point to a role for Reelin in either regulating stem cell quiescence or maintenance.

U2 - 10.1371/journal.pone.0119643

DO - 10.1371/journal.pone.0119643

M3 - SCORING: Journal article

C2 - 25760459

VL - 10

SP - e0119643

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 3

ER -