PortalPublikationenStatic and Dynamic Assays of Cell Adhesion Relevant to the V...

Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

Standard

Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature. / Butler, Lynn M; McGettrick, Helen M; Nash, Gerard B.

in: Methods Mol Biol, Jahrgang 1430, 2016, S. 231-48.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Butler, LM, McGettrick, HM & Nash, GB 2016, 'Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature', Methods Mol Biol, Jg. 1430, S. 231-48. https://doi.org/10.1007/978-1-4939-3628-1_16

APA

Butler, L. M., McGettrick, H. M., & Nash, G. B. (2016). Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature. Methods Mol Biol, 1430, 231-48. https://doi.org/10.1007/978-1-4939-3628-1_16

Vancouver

Butler LM, McGettrick HM, Nash GB. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature. Methods Mol Biol. 2016;1430:231-48. https://doi.org/10.1007/978-1-4939-3628-1_16

Bibtex

@article{ed09cea6281c4b4a90647d14a8a9af55,
title = "Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature",
abstract = "Methods are described for analyzing adhesion of isolated cells (such as leukocytes, tumor cells, or precursor cells) to purified adhesion receptors or cultured endothelial cells. {"}Static{"} assays (where cells are allowed to settle on the adhesive substrates) and flow-based assays (where cells are perfused over the substrates) are compared. Direct observations of the time course of adhesion and migration can be made when purified proteins or endothelial cells are cultured in plates, after cells are allowed to settle onto them for a desired period. In the flow-based assay, cells are perfused through coated glass capillaries, flow-channels incorporating coated plates, or commercially available preformed channels. Again, direct video-microscopic observations are made. In this assay various stages of capture, immobilization, and migration can be followed. In general, the static systems have higher throughput and greatest ease of use, but yield less detailed information, while the flow-based assay is most difficult to set up but is most physiologically relevant if one is interested in the dynamics of adhesion in the vasculature.",
keywords = "Journal Article",
author = "Butler, {Lynn M} and McGettrick, {Helen M} and Nash, {Gerard B}",
year = "2016",
doi = "10.1007/978-1-4939-3628-1_16",
language = "English",
volume = "1430",
pages = "231--48",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

AU - Butler, Lynn M

AU - McGettrick, Helen M

AU - Nash, Gerard B

PY - 2016

Y1 - 2016

N2 - Methods are described for analyzing adhesion of isolated cells (such as leukocytes, tumor cells, or precursor cells) to purified adhesion receptors or cultured endothelial cells. "Static" assays (where cells are allowed to settle on the adhesive substrates) and flow-based assays (where cells are perfused over the substrates) are compared. Direct observations of the time course of adhesion and migration can be made when purified proteins or endothelial cells are cultured in plates, after cells are allowed to settle onto them for a desired period. In the flow-based assay, cells are perfused through coated glass capillaries, flow-channels incorporating coated plates, or commercially available preformed channels. Again, direct video-microscopic observations are made. In this assay various stages of capture, immobilization, and migration can be followed. In general, the static systems have higher throughput and greatest ease of use, but yield less detailed information, while the flow-based assay is most difficult to set up but is most physiologically relevant if one is interested in the dynamics of adhesion in the vasculature.

AB - Methods are described for analyzing adhesion of isolated cells (such as leukocytes, tumor cells, or precursor cells) to purified adhesion receptors or cultured endothelial cells. "Static" assays (where cells are allowed to settle on the adhesive substrates) and flow-based assays (where cells are perfused over the substrates) are compared. Direct observations of the time course of adhesion and migration can be made when purified proteins or endothelial cells are cultured in plates, after cells are allowed to settle onto them for a desired period. In the flow-based assay, cells are perfused through coated glass capillaries, flow-channels incorporating coated plates, or commercially available preformed channels. Again, direct video-microscopic observations are made. In this assay various stages of capture, immobilization, and migration can be followed. In general, the static systems have higher throughput and greatest ease of use, but yield less detailed information, while the flow-based assay is most difficult to set up but is most physiologically relevant if one is interested in the dynamics of adhesion in the vasculature.

KW - Journal Article

U2 - 10.1007/978-1-4939-3628-1_16

DO - 10.1007/978-1-4939-3628-1_16

M3 - SCORING: Journal article

C2 - 27172958

VL - 1430

SP - 231

EP - 248

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -