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SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells.

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SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells. / Smith-Hicks, Constance; Xiao, Bo; Deng, Rongkang; Ji, Yifei; Zhao, Xia; Shepherd, Jason D; Posern, Guido; Kuhl, Dietmar; Huganir, Richard L; Ginty, David D; Worley, Paul F; Linden, David J.

in: NAT NEUROSCI, Jahrgang 13, Nr. 9, 9, 2010, S. 1082-1089.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Smith-Hicks, C, Xiao, B, Deng, R, Ji, Y, Zhao, X, Shepherd, JD, Posern, G, Kuhl, D, Huganir, RL, Ginty, DD, Worley, PF & Linden, DJ 2010, 'SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells.', NAT NEUROSCI, Jg. 13, Nr. 9, 9, S. 1082-1089. <http://www.ncbi.nlm.nih.gov/pubmed/20694003?dopt=Citation>

APA

Smith-Hicks, C., Xiao, B., Deng, R., Ji, Y., Zhao, X., Shepherd, J. D., Posern, G., Kuhl, D., Huganir, R. L., Ginty, D. D., Worley, P. F., & Linden, D. J. (2010). SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells. NAT NEUROSCI, 13(9), 1082-1089. [9]. http://www.ncbi.nlm.nih.gov/pubmed/20694003?dopt=Citation

Vancouver

Smith-Hicks C, Xiao B, Deng R, Ji Y, Zhao X, Shepherd JD et al. SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells. NAT NEUROSCI. 2010;13(9):1082-1089. 9.

Bibtex

@article{cf2cea7397bc454f97cbdf2fe2b43a1f,
title = "SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells.",
abstract = "It has been suggested that gene expression and protein synthesis are required for both long-term memory consolidation and late phases of long-term potentiation and long-term depression (LTD). The necessary genes and the specific transcription factor binding sites in their promoters remain unknown. We found that inhibition of the transcription factor SRF or its cofactor MAL blocked the late phase of LTD in mouse cultured cerebellar Purkinje cells, as did deletion of the immediate early gene Arc. Using neuronal bacterial artificial chromosome (BAC) transfection, we found that, in Arc-/- cells transfected with a wild-type Arc BAC, late-phase LTD was rescued. However, mutation of one SRF-binding site in the Arc promoter (SRE 6.9) blocked this rescue. Co-transfection of wild-type Arc and SRF engineered to bind mutated SRE 6.9 restored late-phase LTD in Arc-/-, SRE 6.9 mutant BAC cells. Thus, SRF binding to SRE 6.9 in the Arc promoter is required for the late phase of cerebellar LTD.",
author = "Constance Smith-Hicks and Bo Xiao and Rongkang Deng and Yifei Ji and Xia Zhao and Shepherd, {Jason D} and Guido Posern and Dietmar Kuhl and Huganir, {Richard L} and Ginty, {David D} and Worley, {Paul F} and Linden, {David J}",
year = "2010",
language = "Deutsch",
volume = "13",
pages = "1082--1089",
journal = "NAT NEUROSCI",
issn = "1097-6256",
publisher = "NATURE PUBLISHING GROUP",
number = "9",

}

RIS

TY - JOUR

T1 - SRF binding to SRE 6.9 in the Arc promoter is essential for LTD in cultured Purkinje cells.

AU - Smith-Hicks, Constance

AU - Xiao, Bo

AU - Deng, Rongkang

AU - Ji, Yifei

AU - Zhao, Xia

AU - Shepherd, Jason D

AU - Posern, Guido

AU - Kuhl, Dietmar

AU - Huganir, Richard L

AU - Ginty, David D

AU - Worley, Paul F

AU - Linden, David J

PY - 2010

Y1 - 2010

N2 - It has been suggested that gene expression and protein synthesis are required for both long-term memory consolidation and late phases of long-term potentiation and long-term depression (LTD). The necessary genes and the specific transcription factor binding sites in their promoters remain unknown. We found that inhibition of the transcription factor SRF or its cofactor MAL blocked the late phase of LTD in mouse cultured cerebellar Purkinje cells, as did deletion of the immediate early gene Arc. Using neuronal bacterial artificial chromosome (BAC) transfection, we found that, in Arc-/- cells transfected with a wild-type Arc BAC, late-phase LTD was rescued. However, mutation of one SRF-binding site in the Arc promoter (SRE 6.9) blocked this rescue. Co-transfection of wild-type Arc and SRF engineered to bind mutated SRE 6.9 restored late-phase LTD in Arc-/-, SRE 6.9 mutant BAC cells. Thus, SRF binding to SRE 6.9 in the Arc promoter is required for the late phase of cerebellar LTD.

AB - It has been suggested that gene expression and protein synthesis are required for both long-term memory consolidation and late phases of long-term potentiation and long-term depression (LTD). The necessary genes and the specific transcription factor binding sites in their promoters remain unknown. We found that inhibition of the transcription factor SRF or its cofactor MAL blocked the late phase of LTD in mouse cultured cerebellar Purkinje cells, as did deletion of the immediate early gene Arc. Using neuronal bacterial artificial chromosome (BAC) transfection, we found that, in Arc-/- cells transfected with a wild-type Arc BAC, late-phase LTD was rescued. However, mutation of one SRF-binding site in the Arc promoter (SRE 6.9) blocked this rescue. Co-transfection of wild-type Arc and SRF engineered to bind mutated SRE 6.9 restored late-phase LTD in Arc-/-, SRE 6.9 mutant BAC cells. Thus, SRF binding to SRE 6.9 in the Arc promoter is required for the late phase of cerebellar LTD.

M3 - SCORING: Zeitschriftenaufsatz

VL - 13

SP - 1082

EP - 1089

JO - NAT NEUROSCI

JF - NAT NEUROSCI

SN - 1097-6256

IS - 9

M1 - 9

ER -