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SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication

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SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication. / Halder, Swagata; Torrecilla, Ignacio; Burkhalter, Martin D; Popović, Marta; Fielden, John; Vaz, Bruno; Oehler, Judith; Pilger, Domenic; Lessel, Davor; Wiseman, Katherine; Singh, Abhay Narayan; Vendrell, Iolanda; Fischer, Roman; Philipp, Melanie; Ramadan, Kristijan.

in: NAT COMMUN, Jahrgang 10, Nr. 1, 17.07.2019, S. 3142.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Halder, S, Torrecilla, I, Burkhalter, MD, Popović, M, Fielden, J, Vaz, B, Oehler, J, Pilger, D, Lessel, D, Wiseman, K, Singh, AN, Vendrell, I, Fischer, R, Philipp, M & Ramadan, K 2019, 'SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication', NAT COMMUN, Jg. 10, Nr. 1, S. 3142. https://doi.org/10.1038/s41467-019-11095-y

APA

Halder, S., Torrecilla, I., Burkhalter, M. D., Popović, M., Fielden, J., Vaz, B., Oehler, J., Pilger, D., Lessel, D., Wiseman, K., Singh, A. N., Vendrell, I., Fischer, R., Philipp, M., & Ramadan, K. (2019). SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication. NAT COMMUN, 10(1), 3142. https://doi.org/10.1038/s41467-019-11095-y

Vancouver

Halder S, Torrecilla I, Burkhalter MD, Popović M, Fielden J, Vaz B et al. SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication. NAT COMMUN. 2019 Jul 17;10(1):3142. https://doi.org/10.1038/s41467-019-11095-y

Bibtex

@article{7dc30fa747974b72bd4667123742a782,
title = "SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication",
abstract = "The SPRTN metalloprotease is essential for DNA-protein crosslink (DPC) repair and DNA replication in vertebrate cells. Cells deficient in SPRTN protease exhibit DPC-induced replication stress and genome instability, manifesting as premature ageing and liver cancer. Here, we provide a body of evidence suggesting that SPRTN activates the ATR-CHK1 phosphorylation signalling cascade during physiological DNA replication by proteolysis-dependent eviction of CHK1 from replicative chromatin. During this process, SPRTN proteolyses the C-terminal/inhibitory part of CHK1, liberating N-terminal CHK1 kinase active fragments. Simultaneously, CHK1 full length and its N-terminal fragments phosphorylate SPRTN at the C-terminal regulatory domain, which stimulates SPRTN recruitment to chromatin to promote unperturbed DNA replication fork progression and DPC repair. Our data suggest that a SPRTN-CHK1 cross-activation loop plays a part in DNA replication and protection from DNA replication stress. Finally, our results with purified components of this pathway further support the proposed model of a SPRTN-CHK1 cross-activation loop.",
author = "Swagata Halder and Ignacio Torrecilla and Burkhalter, {Martin D} and Marta Popovi{\'c} and John Fielden and Bruno Vaz and Judith Oehler and Domenic Pilger and Davor Lessel and Katherine Wiseman and Singh, {Abhay Narayan} and Iolanda Vendrell and Roman Fischer and Melanie Philipp and Kristijan Ramadan",
year = "2019",
month = jul,
day = "17",
doi = "10.1038/s41467-019-11095-y",
language = "English",
volume = "10",
pages = "3142",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication

AU - Halder, Swagata

AU - Torrecilla, Ignacio

AU - Burkhalter, Martin D

AU - Popović, Marta

AU - Fielden, John

AU - Vaz, Bruno

AU - Oehler, Judith

AU - Pilger, Domenic

AU - Lessel, Davor

AU - Wiseman, Katherine

AU - Singh, Abhay Narayan

AU - Vendrell, Iolanda

AU - Fischer, Roman

AU - Philipp, Melanie

AU - Ramadan, Kristijan

PY - 2019/7/17

Y1 - 2019/7/17

N2 - The SPRTN metalloprotease is essential for DNA-protein crosslink (DPC) repair and DNA replication in vertebrate cells. Cells deficient in SPRTN protease exhibit DPC-induced replication stress and genome instability, manifesting as premature ageing and liver cancer. Here, we provide a body of evidence suggesting that SPRTN activates the ATR-CHK1 phosphorylation signalling cascade during physiological DNA replication by proteolysis-dependent eviction of CHK1 from replicative chromatin. During this process, SPRTN proteolyses the C-terminal/inhibitory part of CHK1, liberating N-terminal CHK1 kinase active fragments. Simultaneously, CHK1 full length and its N-terminal fragments phosphorylate SPRTN at the C-terminal regulatory domain, which stimulates SPRTN recruitment to chromatin to promote unperturbed DNA replication fork progression and DPC repair. Our data suggest that a SPRTN-CHK1 cross-activation loop plays a part in DNA replication and protection from DNA replication stress. Finally, our results with purified components of this pathway further support the proposed model of a SPRTN-CHK1 cross-activation loop.

AB - The SPRTN metalloprotease is essential for DNA-protein crosslink (DPC) repair and DNA replication in vertebrate cells. Cells deficient in SPRTN protease exhibit DPC-induced replication stress and genome instability, manifesting as premature ageing and liver cancer. Here, we provide a body of evidence suggesting that SPRTN activates the ATR-CHK1 phosphorylation signalling cascade during physiological DNA replication by proteolysis-dependent eviction of CHK1 from replicative chromatin. During this process, SPRTN proteolyses the C-terminal/inhibitory part of CHK1, liberating N-terminal CHK1 kinase active fragments. Simultaneously, CHK1 full length and its N-terminal fragments phosphorylate SPRTN at the C-terminal regulatory domain, which stimulates SPRTN recruitment to chromatin to promote unperturbed DNA replication fork progression and DPC repair. Our data suggest that a SPRTN-CHK1 cross-activation loop plays a part in DNA replication and protection from DNA replication stress. Finally, our results with purified components of this pathway further support the proposed model of a SPRTN-CHK1 cross-activation loop.

U2 - 10.1038/s41467-019-11095-y

DO - 10.1038/s41467-019-11095-y

M3 - SCORING: Journal article

C2 - 31316063

VL - 10

SP - 3142

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -