Specific de-SUMOylation triggered by acquisition of spatial learning is related to epigenetic changes in the rat hippocampus
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Specific de-SUMOylation triggered by acquisition of spatial learning is related to epigenetic changes in the rat hippocampus. / Castro Gomez, Mario Sergio; Barrera-Ocampo, Alvaro A; Machado-Rodriguez, Gloria; Castro-Alvarez, John F; Glatzel, Markus; Giraldo, Marco; Sepulveda-Falla, Diego.
in: NEUROREPORT, Jahrgang 24, Nr. 17, 04.12.2013, S. 976-81.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Specific de-SUMOylation triggered by acquisition of spatial learning is related to epigenetic changes in the rat hippocampus
AU - Castro Gomez, Mario Sergio
AU - Barrera-Ocampo, Alvaro A
AU - Machado-Rodriguez, Gloria
AU - Castro-Alvarez, John F
AU - Glatzel, Markus
AU - Giraldo, Marco
AU - Sepulveda-Falla, Diego
PY - 2013/12/4
Y1 - 2013/12/4
N2 - Histone acetyltransferase activity by transcriptional cofactors such as CREB-binding protein (CBP) and post-translational modifications by small ubiquitin-like modifier-1 (SUMO-1) have shown to be relevant for synaptic and neuronal activity. Here, we investigate whether SUMOylation of CBP plays a role in spatial learning. We assessed protein levels of CBP/p300, SUMO-1, and CBP SUMOylation in the hippocampi of rats trained on the Morris water maze task. Furthermore, we evaluated the post-translational modifications at Zif268, BDNF, and Arc/Arg3.1 promoters using chromatin immunoprecipitation with anti-Acetyl-Histone H3-Lys14 (H3K14Ac) and SUMO-1. We found that CBP/p300 protein expression is unchanged in animals trained for 7 days. However, H3K14Ac-specific histone acetyltransferase activity showed specific hyperacetylation at promoters of Zif268 and BDNF-pI but not of Arc/Arg3.1 and BDNF-pIV. In naive animals, CBP is selectively SUMOylated and the Arc/Arg3.1 promoter is differentially occupied by SUMO-1, although SUMO-1 levels are unchanged. These results suggest a specific negative regulation by SUMO-1 on CBP function and its effect on epigenetic changes triggered by spatial learning and memory processes.
AB - Histone acetyltransferase activity by transcriptional cofactors such as CREB-binding protein (CBP) and post-translational modifications by small ubiquitin-like modifier-1 (SUMO-1) have shown to be relevant for synaptic and neuronal activity. Here, we investigate whether SUMOylation of CBP plays a role in spatial learning. We assessed protein levels of CBP/p300, SUMO-1, and CBP SUMOylation in the hippocampi of rats trained on the Morris water maze task. Furthermore, we evaluated the post-translational modifications at Zif268, BDNF, and Arc/Arg3.1 promoters using chromatin immunoprecipitation with anti-Acetyl-Histone H3-Lys14 (H3K14Ac) and SUMO-1. We found that CBP/p300 protein expression is unchanged in animals trained for 7 days. However, H3K14Ac-specific histone acetyltransferase activity showed specific hyperacetylation at promoters of Zif268 and BDNF-pI but not of Arc/Arg3.1 and BDNF-pIV. In naive animals, CBP is selectively SUMOylated and the Arc/Arg3.1 promoter is differentially occupied by SUMO-1, although SUMO-1 levels are unchanged. These results suggest a specific negative regulation by SUMO-1 on CBP function and its effect on epigenetic changes triggered by spatial learning and memory processes.
U2 - 10.1097/WNR.0000000000000025
DO - 10.1097/WNR.0000000000000025
M3 - SCORING: Journal article
C2 - 24089016
VL - 24
SP - 976
EP - 981
JO - NEUROREPORT
JF - NEUROREPORT
SN - 0959-4965
IS - 17
ER -
