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Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium

Standard

Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium. / Boecker, Werner; van Horn, Laura; Stenman, Göran; Stürken, Christine; Schumacher, Udo; Loening, Thomas; Liesenfeld, Lukas; Korsching, Eberhard; Gläser, Doreen; Tiemann, Katharina; Buchwalow, Igor.

in: LAB INVEST, Jahrgang 98, Nr. 8, 08.2018, S. 1065-1075.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Boecker, W, van Horn, L, Stenman, G, Stürken, C, Schumacher, U, Loening, T, Liesenfeld, L, Korsching, E, Gläser, D, Tiemann, K & Buchwalow, I 2018, 'Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium', LAB INVEST, Jg. 98, Nr. 8, S. 1065-1075. https://doi.org/10.1038/s41374-018-0054-3

APA

Boecker, W., van Horn, L., Stenman, G., Stürken, C., Schumacher, U., Loening, T., Liesenfeld, L., Korsching, E., Gläser, D., Tiemann, K., & Buchwalow, I. (2018). Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium. LAB INVEST, 98(8), 1065-1075. https://doi.org/10.1038/s41374-018-0054-3

Vancouver

Boecker W, van Horn L, Stenman G, Stürken C, Schumacher U, Loening T et al. Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium. LAB INVEST. 2018 Aug;98(8):1065-1075. https://doi.org/10.1038/s41374-018-0054-3

Bibtex

@article{1e6e9a93134e4727ab6dadc6a9e426a2,
title = "Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium",
abstract = "Understanding the mechanisms regulating human mammary epithelium requires knowledge of the cellular constituents of this tissue. Different and partially contradictory definitions and concepts describing the cellular hierarchy of mammary epithelium have been proposed, including our studies of keratins K5 and/or K14 as markers of progenitor cells. Furthermore, we and others have suggested that the p53 homolog p63 is a marker of human breast epithelial stem cells. In this investigation, we expand our previous studies by testing whether immunohistochemical staining with monospecific anti-keratin antibodies in combination with an antibody against the stem cell marker p63 might help refine the different morphologic phenotypes in normal breast epithelium. We used in situ multilabel staining for p63, different keratins, the myoepithelial marker smooth muscle actin (SMA), the estrogen receptor (ER), and Ki67 to dissect and quantify the cellular components of 16 normal pre- and postmenopausal human breast epithelial tissue samples at the single-cell level. Importantly, we confirm the existence of K5+ only cells and suggest that they, in contrast to the current view, are key luminal precursor cells from which K8/18+ progeny cells evolve. These cells are further modified by the expression of ER and Ki67. We have also identified a population of p63+K5+ cells that are only found in nipple ducts. Based on our findings, we propose a new concept of the cellular hierarchy of human breast epithelium, including K5 luminal lineage progenitors throughout the ductal-lobular axis and p63+K5+ progenitors confined to the nipple ducts.",
keywords = "Journal Article",
author = "Werner Boecker and {van Horn}, Laura and G{\"o}ran Stenman and Christine St{\"u}rken and Udo Schumacher and Thomas Loening and Lukas Liesenfeld and Eberhard Korsching and Doreen Gl{\"a}ser and Katharina Tiemann and Igor Buchwalow",
year = "2018",
month = aug,
doi = "10.1038/s41374-018-0054-3",
language = "English",
volume = "98",
pages = "1065--1075",
journal = "LAB INVEST",
issn = "0023-6837",
publisher = "NATURE PUBLISHING GROUP",
number = "8",

}

RIS

TY - JOUR

T1 - Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium

AU - Boecker, Werner

AU - van Horn, Laura

AU - Stenman, Göran

AU - Stürken, Christine

AU - Schumacher, Udo

AU - Loening, Thomas

AU - Liesenfeld, Lukas

AU - Korsching, Eberhard

AU - Gläser, Doreen

AU - Tiemann, Katharina

AU - Buchwalow, Igor

PY - 2018/8

Y1 - 2018/8

N2 - Understanding the mechanisms regulating human mammary epithelium requires knowledge of the cellular constituents of this tissue. Different and partially contradictory definitions and concepts describing the cellular hierarchy of mammary epithelium have been proposed, including our studies of keratins K5 and/or K14 as markers of progenitor cells. Furthermore, we and others have suggested that the p53 homolog p63 is a marker of human breast epithelial stem cells. In this investigation, we expand our previous studies by testing whether immunohistochemical staining with monospecific anti-keratin antibodies in combination with an antibody against the stem cell marker p63 might help refine the different morphologic phenotypes in normal breast epithelium. We used in situ multilabel staining for p63, different keratins, the myoepithelial marker smooth muscle actin (SMA), the estrogen receptor (ER), and Ki67 to dissect and quantify the cellular components of 16 normal pre- and postmenopausal human breast epithelial tissue samples at the single-cell level. Importantly, we confirm the existence of K5+ only cells and suggest that they, in contrast to the current view, are key luminal precursor cells from which K8/18+ progeny cells evolve. These cells are further modified by the expression of ER and Ki67. We have also identified a population of p63+K5+ cells that are only found in nipple ducts. Based on our findings, we propose a new concept of the cellular hierarchy of human breast epithelium, including K5 luminal lineage progenitors throughout the ductal-lobular axis and p63+K5+ progenitors confined to the nipple ducts.

AB - Understanding the mechanisms regulating human mammary epithelium requires knowledge of the cellular constituents of this tissue. Different and partially contradictory definitions and concepts describing the cellular hierarchy of mammary epithelium have been proposed, including our studies of keratins K5 and/or K14 as markers of progenitor cells. Furthermore, we and others have suggested that the p53 homolog p63 is a marker of human breast epithelial stem cells. In this investigation, we expand our previous studies by testing whether immunohistochemical staining with monospecific anti-keratin antibodies in combination with an antibody against the stem cell marker p63 might help refine the different morphologic phenotypes in normal breast epithelium. We used in situ multilabel staining for p63, different keratins, the myoepithelial marker smooth muscle actin (SMA), the estrogen receptor (ER), and Ki67 to dissect and quantify the cellular components of 16 normal pre- and postmenopausal human breast epithelial tissue samples at the single-cell level. Importantly, we confirm the existence of K5+ only cells and suggest that they, in contrast to the current view, are key luminal precursor cells from which K8/18+ progeny cells evolve. These cells are further modified by the expression of ER and Ki67. We have also identified a population of p63+K5+ cells that are only found in nipple ducts. Based on our findings, we propose a new concept of the cellular hierarchy of human breast epithelium, including K5 luminal lineage progenitors throughout the ductal-lobular axis and p63+K5+ progenitors confined to the nipple ducts.

KW - Journal Article

U2 - 10.1038/s41374-018-0054-3

DO - 10.1038/s41374-018-0054-3

M3 - SCORING: Journal article

C2 - 29743728

VL - 98

SP - 1065

EP - 1075

JO - LAB INVEST

JF - LAB INVEST

SN - 0023-6837

IS - 8

ER -