Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium
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Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium. / Wiech, Thorsten; Stein, Stefan; Lachenmaier, Victoria; Schmitt, Eberhard; Schwarz-Finsterle, Jutta; Wiech, Elisabeth; Hildenbrand, Georg; Werner, Martin; Hausmann, Michael.
in: EUR BIOPHYS J BIOPHY, Jahrgang 38, Nr. 6, 01.07.2009, S. 793-806.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium
AU - Wiech, Thorsten
AU - Stein, Stefan
AU - Lachenmaier, Victoria
AU - Schmitt, Eberhard
AU - Schwarz-Finsterle, Jutta
AU - Wiech, Elisabeth
AU - Hildenbrand, Georg
AU - Werner, Martin
AU - Hausmann, Michael
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Several studies suggest a correlation between genome architecture and gene function. To elucidate mechanisms of gene positioning during cell differentiation and malignant transformation we investigated the nuclear positions of the BCL2 alleles and chromosome 18 territories in different layers of nonneoplastic cervical squamous epithelium and cervical squamous carcinomas in relation to gene expression. Fluorescence in situ hybridization and three-dimensional (3D) image analysis using tissue sections revealed that one BCL2 allele was located more peripherally than the other one in nuclei of the basal layer of nonneoplastic epithelium. During terminal cell differentiation the outer BCL2 allele showed a shift towards the nuclear center. In BCL2-expressing carcinomas the inner BCL2 allele was located more peripherally compared with the basal layer of nonneoplastic epithelium. Our results suggest a functional relevance of unequal allelic BCL2 gene positioning and support the hypothesis that transcriptional BCL2 activation is associated with BCL2 relocation towards the nuclear periphery.
AB - Several studies suggest a correlation between genome architecture and gene function. To elucidate mechanisms of gene positioning during cell differentiation and malignant transformation we investigated the nuclear positions of the BCL2 alleles and chromosome 18 territories in different layers of nonneoplastic cervical squamous epithelium and cervical squamous carcinomas in relation to gene expression. Fluorescence in situ hybridization and three-dimensional (3D) image analysis using tissue sections revealed that one BCL2 allele was located more peripherally than the other one in nuclei of the basal layer of nonneoplastic epithelium. During terminal cell differentiation the outer BCL2 allele showed a shift towards the nuclear center. In BCL2-expressing carcinomas the inner BCL2 allele was located more peripherally compared with the basal layer of nonneoplastic epithelium. Our results suggest a functional relevance of unequal allelic BCL2 gene positioning and support the hypothesis that transcriptional BCL2 activation is associated with BCL2 relocation towards the nuclear periphery.
KW - Alleles
KW - Cell Differentiation
KW - Cell Nucleus
KW - Cervix Uteri
KW - Chromosomes, Human, Pair 18
KW - Epithelium
KW - Female
KW - Gene Expression
KW - Genes, bcl-2
KW - Humans
KW - Imaging, Three-Dimensional
KW - Immunohistochemistry
KW - In Situ Hybridization, Fluorescence
KW - Microarray Analysis
KW - Microscopy, Fluorescence
KW - Neoplasms, Squamous Cell
KW - Uterine Cervical Neoplasms
U2 - 10.1007/s00249-009-0474-5
DO - 10.1007/s00249-009-0474-5
M3 - SCORING: Journal article
C2 - 19495739
VL - 38
SP - 793
EP - 806
JO - EUR BIOPHYS J BIOPHY
JF - EUR BIOPHYS J BIOPHY
SN - 0175-7571
IS - 6
ER -
