PortalPublikationenSocial exclusion changes histone modifications H3K4me3 and H...

Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice

Standard

Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice. / Krause, Linda; Haubold, Bernhard; Börsch-Haubold, Angelika G.

in: PLOS ONE, Jahrgang 10, Nr. 8, 2015, S. e0133988.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Krause, L, Haubold, B & Börsch-Haubold, AG 2015, 'Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice', PLOS ONE, Jg. 10, Nr. 8, S. e0133988. https://doi.org/10.1371/journal.pone.0133988

APA

Krause, L., Haubold, B., & Börsch-Haubold, A. G. (2015). Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice. PLOS ONE, 10(8), e0133988. https://doi.org/10.1371/journal.pone.0133988

Vancouver

Krause L, Haubold B, Börsch-Haubold AG. Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice. PLOS ONE. 2015;10(8):e0133988. https://doi.org/10.1371/journal.pone.0133988

Bibtex

@article{5e3434ac77e94e7dae3444660714686a,
title = "Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice",
abstract = "Wild house mice form social hierarchies with aggressive males defending territories, in which females, young mice and submissive adult males share nests. In contrast, socially excluded males are barred from breeding groups, have numerous bite wounds and patches of thinning fur. Since their feeding times are often disrupted, we investigated whether social exclusion leads to changes in epigenetic marks of metabolic genes in liver tissue. We used chromatin immunoprecipitation and quantitative PCR to measure enrichment of two activating histone marks at 15 candidate loci. The epigenetic profiles of healthy males sampled from nest boxes differed significantly from the profiles of ostracized males caught outside of nests and showing bite wounds indicative of social exclusion. Enrichment of histone-3 lysine-4 trimethylation (H3K4me3) changed significantly at genes Cyp4a14, Gapdh, Nr3c1, Pck1, Ppara, and Sqle. Changes at histone-3 lysine-27 acetylation (H3K27ac) marks were detected at genes Fasn, Nr3c1, and Plin5. A principal components analysis separated the socialized from the ostracized mice. This was independent of body weight for the H3K4me3 mark, and partially dependent for H3K27ac. There was no separation, however, between healthy males that had been sampled from two different nests. A hierarchical cluster analysis also separated the two phenotypes, which was independent of body weight for both markers. Our study shows that a period of social exclusion during adult life leads to quantitative changes in histone modification patterns in mouse liver tissue. Similar epigenetic changes might occur during the development of stress-induced metabolic disorders in humans. ",
keywords = "Animals, Chromatin Immunoprecipitation, DNA Methylation, Epigenesis, Genetic, Feeding Behavior, Female, Hierarchy, Social, Histones, Humans, Liver, Lysine, Male, Metabolic Diseases, Mice, Promoter Regions, Genetic, Journal Article, Research Support, Non-U.S. Gov't",
author = "Linda Krause and Bernhard Haubold and B{\"o}rsch-Haubold, {Angelika G}",
year = "2015",
doi = "10.1371/journal.pone.0133988",
language = "English",
volume = "10",
pages = "e0133988",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Social exclusion changes histone modifications H3K4me3 and H3K27ac in liver tissue of wild house mice

AU - Krause, Linda

AU - Haubold, Bernhard

AU - Börsch-Haubold, Angelika G

PY - 2015

Y1 - 2015

N2 - Wild house mice form social hierarchies with aggressive males defending territories, in which females, young mice and submissive adult males share nests. In contrast, socially excluded males are barred from breeding groups, have numerous bite wounds and patches of thinning fur. Since their feeding times are often disrupted, we investigated whether social exclusion leads to changes in epigenetic marks of metabolic genes in liver tissue. We used chromatin immunoprecipitation and quantitative PCR to measure enrichment of two activating histone marks at 15 candidate loci. The epigenetic profiles of healthy males sampled from nest boxes differed significantly from the profiles of ostracized males caught outside of nests and showing bite wounds indicative of social exclusion. Enrichment of histone-3 lysine-4 trimethylation (H3K4me3) changed significantly at genes Cyp4a14, Gapdh, Nr3c1, Pck1, Ppara, and Sqle. Changes at histone-3 lysine-27 acetylation (H3K27ac) marks were detected at genes Fasn, Nr3c1, and Plin5. A principal components analysis separated the socialized from the ostracized mice. This was independent of body weight for the H3K4me3 mark, and partially dependent for H3K27ac. There was no separation, however, between healthy males that had been sampled from two different nests. A hierarchical cluster analysis also separated the two phenotypes, which was independent of body weight for both markers. Our study shows that a period of social exclusion during adult life leads to quantitative changes in histone modification patterns in mouse liver tissue. Similar epigenetic changes might occur during the development of stress-induced metabolic disorders in humans.

AB - Wild house mice form social hierarchies with aggressive males defending territories, in which females, young mice and submissive adult males share nests. In contrast, socially excluded males are barred from breeding groups, have numerous bite wounds and patches of thinning fur. Since their feeding times are often disrupted, we investigated whether social exclusion leads to changes in epigenetic marks of metabolic genes in liver tissue. We used chromatin immunoprecipitation and quantitative PCR to measure enrichment of two activating histone marks at 15 candidate loci. The epigenetic profiles of healthy males sampled from nest boxes differed significantly from the profiles of ostracized males caught outside of nests and showing bite wounds indicative of social exclusion. Enrichment of histone-3 lysine-4 trimethylation (H3K4me3) changed significantly at genes Cyp4a14, Gapdh, Nr3c1, Pck1, Ppara, and Sqle. Changes at histone-3 lysine-27 acetylation (H3K27ac) marks were detected at genes Fasn, Nr3c1, and Plin5. A principal components analysis separated the socialized from the ostracized mice. This was independent of body weight for the H3K4me3 mark, and partially dependent for H3K27ac. There was no separation, however, between healthy males that had been sampled from two different nests. A hierarchical cluster analysis also separated the two phenotypes, which was independent of body weight for both markers. Our study shows that a period of social exclusion during adult life leads to quantitative changes in histone modification patterns in mouse liver tissue. Similar epigenetic changes might occur during the development of stress-induced metabolic disorders in humans.

KW - Animals

KW - Chromatin Immunoprecipitation

KW - DNA Methylation

KW - Epigenesis, Genetic

KW - Feeding Behavior

KW - Female

KW - Hierarchy, Social

KW - Histones

KW - Humans

KW - Liver

KW - Lysine

KW - Male

KW - Metabolic Diseases

KW - Mice

KW - Promoter Regions, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0133988

DO - 10.1371/journal.pone.0133988

M3 - SCORING: Journal article

C2 - 26267652

VL - 10

SP - e0133988

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 8

ER -