Shear-Induced Unfolding and Enzymatic Cleavage of Full-Length VWF Multimers
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Shear-Induced Unfolding and Enzymatic Cleavage of Full-Length VWF Multimers. / Lippok, Svenja; Radtke, Matthias; Obser, Tobias; Kleemeier, Lars; Schneppenheim, Reinhard; Budde, Ulrich; Netz, Roland R; Rädler, Joachim O.
in: BIOPHYS J, Jahrgang 110, Nr. 3, 02.02.2016, S. 545-54.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Shear-Induced Unfolding and Enzymatic Cleavage of Full-Length VWF Multimers
AU - Lippok, Svenja
AU - Radtke, Matthias
AU - Obser, Tobias
AU - Kleemeier, Lars
AU - Schneppenheim, Reinhard
AU - Budde, Ulrich
AU - Netz, Roland R
AU - Rädler, Joachim O
N1 - Copyright © 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved.
PY - 2016/2/2
Y1 - 2016/2/2
N2 - Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy (FCS) and a microfluidic shear cell to monitor real-time kinetics of full-length VWF proteolysis as a function of shear stress. For comparison, we also measure the Michaelis-Menten kinetics of ADAMTS13 cleavage of wild-type VWF in the absence of shear but partially denaturing conditions. Under shear, ADAMTS13 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multimer analysis. In agreement with Brownian hydrodynamics simulations, we find a sigmoidal increase of the enzymatic rate as a function of shear at a threshold shear rate γ˙1/2 = 5522/s. The same flow-rate dependence of ADAMTS13 activity we also observe in blood plasma, which is relevant to predict hemostatic dysfunction.
AB - Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy (FCS) and a microfluidic shear cell to monitor real-time kinetics of full-length VWF proteolysis as a function of shear stress. For comparison, we also measure the Michaelis-Menten kinetics of ADAMTS13 cleavage of wild-type VWF in the absence of shear but partially denaturing conditions. Under shear, ADAMTS13 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multimer analysis. In agreement with Brownian hydrodynamics simulations, we find a sigmoidal increase of the enzymatic rate as a function of shear at a threshold shear rate γ˙1/2 = 5522/s. The same flow-rate dependence of ADAMTS13 activity we also observe in blood plasma, which is relevant to predict hemostatic dysfunction.
U2 - 10.1016/j.bpj.2015.12.023
DO - 10.1016/j.bpj.2015.12.023
M3 - SCORING: Journal article
C2 - 26840720
VL - 110
SP - 545
EP - 554
JO - BIOPHYS J
JF - BIOPHYS J
SN - 0006-3495
IS - 3
ER -