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SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D.

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SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D. / Rexhepaj, Rexhep; Alesutan, Ioana; Gu, Shuchen; Pelzl, Lisann; Eichenmüller, Melanie; Pathare, Ganesh; Föller, Michael; Kuhl, Dietmar; Lang, Florian.

in: PFLUG ARCH EUR J PHY, Jahrgang 462, Nr. 3, 3, 2011, S. 489-494.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rexhepaj, R, Alesutan, I, Gu, S, Pelzl, L, Eichenmüller, M, Pathare, G, Föller, M, Kuhl, D & Lang, F 2011, 'SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D.', PFLUG ARCH EUR J PHY, Jg. 462, Nr. 3, 3, S. 489-494. <http://www.ncbi.nlm.nih.gov/pubmed/21735060?dopt=Citation>

APA

Rexhepaj, R., Alesutan, I., Gu, S., Pelzl, L., Eichenmüller, M., Pathare, G., Föller, M., Kuhl, D., & Lang, F. (2011). SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D. PFLUG ARCH EUR J PHY, 462(3), 489-494. [3]. http://www.ncbi.nlm.nih.gov/pubmed/21735060?dopt=Citation

Vancouver

Rexhepaj R, Alesutan I, Gu S, Pelzl L, Eichenmüller M, Pathare G et al. SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D. PFLUG ARCH EUR J PHY. 2011;462(3):489-494. 3.

Bibtex

@article{1790f1c703944c7aa5e999b31b74a892,
title = "SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D.",
abstract = "The serum- and glucocorticoid-inducible kinase SGK1 has previously been shown to mediate the glucocorticoid-dependent stimulation of several intestinal transport systems including the electrogenic glucose transporter SGLT1. In squamous carcinoma cells, SGK1 expression is stimulated by 1,25(OH)?D?, the biologically active metabolite of vitamin D. The present study explored whether vitamin D influences the intestinal SGLT1 activity. Jejunal SGLT1 activity was determined by Ussing chamber experiments. Under a normal diet, the electrogenic glucose transport was similar in SGK1 knockout (sgk1 ( -/- )) and wild type mice (sgk1 ( +/+ )). Following a vitamin D-rich diet (14 days 10,000 I.U. vitamin D), the SGK1 transcript levels as well as the SGLT1 protein abundance were increased in sgk1(+/+) mice. Moreover, SGLT1 activity was increased in sgk1(+/+) mice but not in sgk1(-/-) mice following a vitamin D-rich diet. Furthermore, an oral glucose load was followed by an increase in the plasma glucose concentration to significantly higher values in sgk1(+/+) mice treated with a vitamin D-rich diet than in untreated sgk1(+/+) mice. In conclusion, vitamin D treatment upregulates the expression of SGK1, which in turn enhances SGLT1 activity.",
keywords = "Animals, Humans, Mice, Mice, Knockout, Diet, Glucose Tolerance Test, Glucose/metabolism, Caco-2 Cells, Immediate-Early Proteins/genetics/*metabolism, Intestines/*metabolism, Protein-Serine-Threonine Kinases/genetics/*metabolism, Sodium-Glucose Transporter 1/genetics/*metabolism, Vitamin D/*administration & dosage, Animals, Humans, Mice, Mice, Knockout, Diet, Glucose Tolerance Test, Glucose/metabolism, Caco-2 Cells, Immediate-Early Proteins/genetics/*metabolism, Intestines/*metabolism, Protein-Serine-Threonine Kinases/genetics/*metabolism, Sodium-Glucose Transporter 1/genetics/*metabolism, Vitamin D/*administration & dosage",
author = "Rexhep Rexhepaj and Ioana Alesutan and Shuchen Gu and Lisann Pelzl and Melanie Eichenm{\"u}ller and Ganesh Pathare and Michael F{\"o}ller and Dietmar Kuhl and Florian Lang",
year = "2011",
language = "English",
volume = "462",
pages = "489--494",
journal = "PFLUG ARCH EUR J PHY",
issn = "0031-6768",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - SGK1-dependent stimulation of intestinal SGLT1 activity by vitamin D.

AU - Rexhepaj, Rexhep

AU - Alesutan, Ioana

AU - Gu, Shuchen

AU - Pelzl, Lisann

AU - Eichenmüller, Melanie

AU - Pathare, Ganesh

AU - Föller, Michael

AU - Kuhl, Dietmar

AU - Lang, Florian

PY - 2011

Y1 - 2011

N2 - The serum- and glucocorticoid-inducible kinase SGK1 has previously been shown to mediate the glucocorticoid-dependent stimulation of several intestinal transport systems including the electrogenic glucose transporter SGLT1. In squamous carcinoma cells, SGK1 expression is stimulated by 1,25(OH)?D?, the biologically active metabolite of vitamin D. The present study explored whether vitamin D influences the intestinal SGLT1 activity. Jejunal SGLT1 activity was determined by Ussing chamber experiments. Under a normal diet, the electrogenic glucose transport was similar in SGK1 knockout (sgk1 ( -/- )) and wild type mice (sgk1 ( +/+ )). Following a vitamin D-rich diet (14 days 10,000 I.U. vitamin D), the SGK1 transcript levels as well as the SGLT1 protein abundance were increased in sgk1(+/+) mice. Moreover, SGLT1 activity was increased in sgk1(+/+) mice but not in sgk1(-/-) mice following a vitamin D-rich diet. Furthermore, an oral glucose load was followed by an increase in the plasma glucose concentration to significantly higher values in sgk1(+/+) mice treated with a vitamin D-rich diet than in untreated sgk1(+/+) mice. In conclusion, vitamin D treatment upregulates the expression of SGK1, which in turn enhances SGLT1 activity.

AB - The serum- and glucocorticoid-inducible kinase SGK1 has previously been shown to mediate the glucocorticoid-dependent stimulation of several intestinal transport systems including the electrogenic glucose transporter SGLT1. In squamous carcinoma cells, SGK1 expression is stimulated by 1,25(OH)?D?, the biologically active metabolite of vitamin D. The present study explored whether vitamin D influences the intestinal SGLT1 activity. Jejunal SGLT1 activity was determined by Ussing chamber experiments. Under a normal diet, the electrogenic glucose transport was similar in SGK1 knockout (sgk1 ( -/- )) and wild type mice (sgk1 ( +/+ )). Following a vitamin D-rich diet (14 days 10,000 I.U. vitamin D), the SGK1 transcript levels as well as the SGLT1 protein abundance were increased in sgk1(+/+) mice. Moreover, SGLT1 activity was increased in sgk1(+/+) mice but not in sgk1(-/-) mice following a vitamin D-rich diet. Furthermore, an oral glucose load was followed by an increase in the plasma glucose concentration to significantly higher values in sgk1(+/+) mice treated with a vitamin D-rich diet than in untreated sgk1(+/+) mice. In conclusion, vitamin D treatment upregulates the expression of SGK1, which in turn enhances SGLT1 activity.

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Knockout

KW - Diet

KW - Glucose Tolerance Test

KW - Glucose/metabolism

KW - Caco-2 Cells

KW - Immediate-Early Proteins/genetics/metabolism

KW - Intestines/metabolism

KW - Protein-Serine-Threonine Kinases/genetics/metabolism

KW - Sodium-Glucose Transporter 1/genetics/metabolism

KW - Vitamin D/administration & dosage

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Knockout

KW - Diet

KW - Glucose Tolerance Test

KW - Glucose/metabolism

KW - Caco-2 Cells

KW - Immediate-Early Proteins/genetics/metabolism

KW - Intestines/metabolism

KW - Protein-Serine-Threonine Kinases/genetics/metabolism

KW - Sodium-Glucose Transporter 1/genetics/metabolism

KW - Vitamin D/administration & dosage

M3 - SCORING: Journal article

VL - 462

SP - 489

EP - 494

JO - PFLUG ARCH EUR J PHY

JF - PFLUG ARCH EUR J PHY

SN - 0031-6768

IS - 3

M1 - 3

ER -