Serum Shiga toxin 2 values in patients during acute phase of diarrhoea-associated haemolytic uraemic syndrome
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Serum Shiga toxin 2 values in patients during acute phase of diarrhoea-associated haemolytic uraemic syndrome. / He, Xiaohua; Quiñones, Beatriz; Loo, Maroeska Te; Loos, Sebastian; Scavia, Gaia; Brigotti, Maurizio; Levtchenko, Elena; Monnens, Leo.
in: ACTA PAEDIATR, Jahrgang 104, Nr. 12, 12.2015, S. e564-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Serum Shiga toxin 2 values in patients during acute phase of diarrhoea-associated haemolytic uraemic syndrome
AU - He, Xiaohua
AU - Quiñones, Beatriz
AU - Loo, Maroeska Te
AU - Loos, Sebastian
AU - Scavia, Gaia
AU - Brigotti, Maurizio
AU - Levtchenko, Elena
AU - Monnens, Leo
N1 - ©2015 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.
PY - 2015/12
Y1 - 2015/12
N2 - AIM: Shiga toxins are delivered via systemic circulation and are considered to be the cause of diarrhoea-associated haemolytic uraemic syndrome (HUS), as they injure endothelial cells, particularly in the glomeruli. This study measured Shiga toxin 2 (Stx2) in the serum of children affected in by HUS due to Stx2 producing Escherichia coli.METHODS: The concentration of free Stx2 was measured in the serum of 16 children, collected immediately after admission to the clinic in the acute phase of HUS, using a sandwich enzyme-linked immunosorbent assay. The family members of two children were also investigated, with the relative toxicity of Stx2 assessed by a Vero cell-based fluorescent assay.RESULTS: Stx2 was found in the serum of eight of the 16 children who were investigated. It was also detected in four of the six family members not showing symptomatic HUS, with an extremely high level in two.CONCLUSION: An absent or rather low concentration of Stx2 was found in the serum of children admitted to the clinic with diarrhoea-associated HUS. The high concentration of Stx2 in family members without HUS, but mostly with watery diarrhoea and raised functional activity, was in line with the concept of early injury by Stx2.
AB - AIM: Shiga toxins are delivered via systemic circulation and are considered to be the cause of diarrhoea-associated haemolytic uraemic syndrome (HUS), as they injure endothelial cells, particularly in the glomeruli. This study measured Shiga toxin 2 (Stx2) in the serum of children affected in by HUS due to Stx2 producing Escherichia coli.METHODS: The concentration of free Stx2 was measured in the serum of 16 children, collected immediately after admission to the clinic in the acute phase of HUS, using a sandwich enzyme-linked immunosorbent assay. The family members of two children were also investigated, with the relative toxicity of Stx2 assessed by a Vero cell-based fluorescent assay.RESULTS: Stx2 was found in the serum of eight of the 16 children who were investigated. It was also detected in four of the six family members not showing symptomatic HUS, with an extremely high level in two.CONCLUSION: An absent or rather low concentration of Stx2 was found in the serum of children admitted to the clinic with diarrhoea-associated HUS. The high concentration of Stx2 in family members without HUS, but mostly with watery diarrhoea and raised functional activity, was in line with the concept of early injury by Stx2.
KW - Adolescent
KW - Animals
KW - Blood Chemical Analysis
KW - Cercopithecus aethiops
KW - Child
KW - Child, Preschool
KW - Cohort Studies
KW - Female
KW - Hemolytic-Uremic Syndrome
KW - Humans
KW - Infant
KW - Male
KW - Shiga Toxin 2
KW - Vero Cells
KW - Evaluation Studies
KW - Journal Article
U2 - 10.1111/apa.13211
DO - 10.1111/apa.13211
M3 - SCORING: Journal article
C2 - 26361237
VL - 104
SP - e564-8
JO - ACTA PAEDIATR
JF - ACTA PAEDIATR
SN - 0803-5253
IS - 12
ER -
