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Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation.

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Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation. / Fang, Lubin; Lange, Claudia; Engel, Melanie; Zander, Axel R.; Fehse, Boris.

in: TRANSPLANTATION, Jahrgang 82, Nr. 10, 10, 2006, S. 1370-1373.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fang, L, Lange, C, Engel, M, Zander, AR & Fehse, B 2006, 'Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation.', TRANSPLANTATION, Jg. 82, Nr. 10, 10, S. 1370-1373. <http://www.ncbi.nlm.nih.gov/pubmed/17130787?dopt=Citation>

APA

Fang, L., Lange, C., Engel, M., Zander, A. R., & Fehse, B. (2006). Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation. TRANSPLANTATION, 82(10), 1370-1373. [10]. http://www.ncbi.nlm.nih.gov/pubmed/17130787?dopt=Citation

Vancouver

Fang L, Lange C, Engel M, Zander AR, Fehse B. Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation. TRANSPLANTATION. 2006;82(10):1370-1373. 10.

Bibtex

@article{a9fcd5112035449cb754468b450f47ff,
title = "Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation.",
abstract = "The human leukocyte antigen-independent immune-modulatory potential of human mesenchymal stem cells (hMSC) makes them a promising candidate for clinical cell therapy. A better understanding of their {"}immune-privileged{"} status is therefore of high priority. Here we used Ki-67-antigen staining to estimate T-cell alloreactivity in mixed lymphocyte cultures in the presence of hMSC as second or third party. We found that the allostimulatory activity of mesenchymal stem cells (MSC) leading to an increased T-cell proliferation and interleukin-2 secretion is measurable only at low MSC/effector ratios (<or =0.1:1). Moreover, this stimulating effect could be efficiently suppressed by MSC-conditioned medium. This suggests that the {"}immune-privileged{"} status of MSC exists only when MSC-mediated downregulation of immune cell activation can overrule their own allostimulatory potential. Thus the {"}immune-privileged{"} state of MSC represents a sensitive balance of suppressing and activating effects, which should be considered in a clinical setting with limited cell amounts.",
author = "Lubin Fang and Claudia Lange and Melanie Engel and Zander, {Axel R.} and Boris Fehse",
year = "2006",
language = "Deutsch",
volume = "82",
pages = "1370--1373",
journal = "TRANSPLANTATION",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

RIS

TY - JOUR

T1 - Sensitive balance of suppressing and activating effects of mesenchymal stem cells on T-cell proliferation.

AU - Fang, Lubin

AU - Lange, Claudia

AU - Engel, Melanie

AU - Zander, Axel R.

AU - Fehse, Boris

PY - 2006

Y1 - 2006

N2 - The human leukocyte antigen-independent immune-modulatory potential of human mesenchymal stem cells (hMSC) makes them a promising candidate for clinical cell therapy. A better understanding of their "immune-privileged" status is therefore of high priority. Here we used Ki-67-antigen staining to estimate T-cell alloreactivity in mixed lymphocyte cultures in the presence of hMSC as second or third party. We found that the allostimulatory activity of mesenchymal stem cells (MSC) leading to an increased T-cell proliferation and interleukin-2 secretion is measurable only at low MSC/effector ratios (<or =0.1:1). Moreover, this stimulating effect could be efficiently suppressed by MSC-conditioned medium. This suggests that the "immune-privileged" status of MSC exists only when MSC-mediated downregulation of immune cell activation can overrule their own allostimulatory potential. Thus the "immune-privileged" state of MSC represents a sensitive balance of suppressing and activating effects, which should be considered in a clinical setting with limited cell amounts.

AB - The human leukocyte antigen-independent immune-modulatory potential of human mesenchymal stem cells (hMSC) makes them a promising candidate for clinical cell therapy. A better understanding of their "immune-privileged" status is therefore of high priority. Here we used Ki-67-antigen staining to estimate T-cell alloreactivity in mixed lymphocyte cultures in the presence of hMSC as second or third party. We found that the allostimulatory activity of mesenchymal stem cells (MSC) leading to an increased T-cell proliferation and interleukin-2 secretion is measurable only at low MSC/effector ratios (<or =0.1:1). Moreover, this stimulating effect could be efficiently suppressed by MSC-conditioned medium. This suggests that the "immune-privileged" status of MSC exists only when MSC-mediated downregulation of immune cell activation can overrule their own allostimulatory potential. Thus the "immune-privileged" state of MSC represents a sensitive balance of suppressing and activating effects, which should be considered in a clinical setting with limited cell amounts.

M3 - SCORING: Zeitschriftenaufsatz

VL - 82

SP - 1370

EP - 1373

JO - TRANSPLANTATION

JF - TRANSPLANTATION

SN - 0041-1337

IS - 10

M1 - 10

ER -