PortalPublikationenSelective optogenetic control of Gq signaling using human Ne...

Selective optogenetic control of Gq signaling using human Neuropsin

Standard

Selective optogenetic control of Gq signaling using human Neuropsin. / Wagdi, Ahmed; Malan, Daniela; Sathyanarayanan, Udhayabhaskar; Beauchamp, Janosch S; Vogt, Markus; Zipf, David; Beiert, Thomas; Mansuroglu, Berivan; Dusend, Vanessa; Meininghaus, Mark; Schneider, Linn; Kalthof, Bernd; Wiegert, J Simon; König, Gabriele M; Kostenis, Evi; Patejdl, Robert; Sasse, Philipp; Bruegmann, Tobias.

in: NAT COMMUN, Jahrgang 13, Nr. 1, 1765, 01.04.2022.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wagdi, A, Malan, D, Sathyanarayanan, U, Beauchamp, JS, Vogt, M, Zipf, D, Beiert, T, Mansuroglu, B, Dusend, V, Meininghaus, M, Schneider, L, Kalthof, B, Wiegert, JS, König, GM, Kostenis, E, Patejdl, R, Sasse, P & Bruegmann, T 2022, 'Selective optogenetic control of Gq signaling using human Neuropsin', NAT COMMUN, Jg. 13, Nr. 1, 1765. https://doi.org/10.1038/s41467-022-29265-w

APA

Wagdi, A., Malan, D., Sathyanarayanan, U., Beauchamp, J. S., Vogt, M., Zipf, D., Beiert, T., Mansuroglu, B., Dusend, V., Meininghaus, M., Schneider, L., Kalthof, B., Wiegert, J. S., König, G. M., Kostenis, E., Patejdl, R., Sasse, P., & Bruegmann, T. (2022). Selective optogenetic control of Gq signaling using human Neuropsin. NAT COMMUN, 13(1), [1765]. https://doi.org/10.1038/s41467-022-29265-w

Vancouver

Wagdi A, Malan D, Sathyanarayanan U, Beauchamp JS, Vogt M, Zipf D et al. Selective optogenetic control of Gq signaling using human Neuropsin. NAT COMMUN. 2022 Apr 1;13(1). 1765. https://doi.org/10.1038/s41467-022-29265-w

Bibtex

@article{d985ca43a3af4c4b8e8b64e013915c1a,
title = "Selective optogenetic control of Gq signaling using human Neuropsin",
abstract = "Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.",
author = "Ahmed Wagdi and Daniela Malan and Udhayabhaskar Sathyanarayanan and Beauchamp, {Janosch S} and Markus Vogt and David Zipf and Thomas Beiert and Berivan Mansuroglu and Vanessa Dusend and Mark Meininghaus and Linn Schneider and Bernd Kalthof and Wiegert, {J Simon} and K{\"o}nig, {Gabriele M} and Evi Kostenis and Robert Patejdl and Philipp Sasse and Tobias Bruegmann",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
month = apr,
day = "1",
doi = "10.1038/s41467-022-29265-w",
language = "English",
volume = "13",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Selective optogenetic control of Gq signaling using human Neuropsin

AU - Wagdi, Ahmed

AU - Malan, Daniela

AU - Sathyanarayanan, Udhayabhaskar

AU - Beauchamp, Janosch S

AU - Vogt, Markus

AU - Zipf, David

AU - Beiert, Thomas

AU - Mansuroglu, Berivan

AU - Dusend, Vanessa

AU - Meininghaus, Mark

AU - Schneider, Linn

AU - Kalthof, Bernd

AU - Wiegert, J Simon

AU - König, Gabriele M

AU - Kostenis, Evi

AU - Patejdl, Robert

AU - Sasse, Philipp

AU - Bruegmann, Tobias

N1 - © 2022. The Author(s).

PY - 2022/4/1

Y1 - 2022/4/1

N2 - Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.

AB - Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.

U2 - 10.1038/s41467-022-29265-w

DO - 10.1038/s41467-022-29265-w

M3 - SCORING: Journal article

C2 - 35365606

VL - 13

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

M1 - 1765

ER -