Screening for circulating nucleic acids and caspase activity in the peripheral blood as potential diagnostic tools in lung cancer.
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Screening for circulating nucleic acids and caspase activity in the peripheral blood as potential diagnostic tools in lung cancer. / Roth, Carina; Kasimir-Bauer, Sabine; Pantel, Klaus; Schwarzenbach, Heidi.
in: MOL ONCOL, Jahrgang 5, Nr. 3, 3, 2011, S. 281-291.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Screening for circulating nucleic acids and caspase activity in the peripheral blood as potential diagnostic tools in lung cancer.
AU - Roth, Carina
AU - Kasimir-Bauer, Sabine
AU - Pantel, Klaus
AU - Schwarzenbach, Heidi
PY - 2011
Y1 - 2011
N2 - The focus of the current investigational study was to examine whether circulating nucleic acids (i.e., DNA and microRNAs) have the potential to become suitable blood-based markers for diagnosis and progression of lung cancer. The concentrations of cell-free DNA and four circulating microRNAs (miR10b, miR34a, miR141 and miR155) as well as the caspase activity were measured in serum of 35 lung cancer patients (19 non-small-cell lung cancer, 8 small cell lung cancer patients and 8 patients with indefinite cancer type), 7 patients with benign lung tumors and 28 healthy individuals by PicoGreen, TaqMan MicroRNA, and Caspase-Glo®3/7 assay, respectively. The data were correlated with the established risk factors for lung cancer progression. The concentrations of cell-free DNA (p = 0.0001), serum microRNAs (p = 0.0001) and caspase activities (p = 0.0001) significantly discriminated cancer patients from healthy individuals. Serum DNA, caspase activities and RNA levels could not distinguish between patients with benign lung disease and cancer patients. However, the levels of miR10b (p = 0.002), miR141 (p = 0.0001) and miR155 (p = 0.007) were significantly higher in lung cancer patients than those in patients with benign disease. As determined by the Spearman-Rho test, high levels of cell-free DNA significantly correlated with elevated circulating caspase activities (p = 0.0001). In lung cancer patients high serum miR10b values associated with lymph node metastasis (p <0.03) and elevated levels of TPA (tissue polypeptide antigen, p = 0.01), whereas high serum miR141 values associated with elevated levels of uPA (urokinase plasminogen activator, p = 0.02). The findings of our pilot study suggest that the assays for circulating DNA, microRNAs and caspase activities in blood might become novel minimally invasive diagnostic tools for detection and risk assessment of lung cancer, provided that their clinical utility can be confirmed in larger prospective trials.
AB - The focus of the current investigational study was to examine whether circulating nucleic acids (i.e., DNA and microRNAs) have the potential to become suitable blood-based markers for diagnosis and progression of lung cancer. The concentrations of cell-free DNA and four circulating microRNAs (miR10b, miR34a, miR141 and miR155) as well as the caspase activity were measured in serum of 35 lung cancer patients (19 non-small-cell lung cancer, 8 small cell lung cancer patients and 8 patients with indefinite cancer type), 7 patients with benign lung tumors and 28 healthy individuals by PicoGreen, TaqMan MicroRNA, and Caspase-Glo®3/7 assay, respectively. The data were correlated with the established risk factors for lung cancer progression. The concentrations of cell-free DNA (p = 0.0001), serum microRNAs (p = 0.0001) and caspase activities (p = 0.0001) significantly discriminated cancer patients from healthy individuals. Serum DNA, caspase activities and RNA levels could not distinguish between patients with benign lung disease and cancer patients. However, the levels of miR10b (p = 0.002), miR141 (p = 0.0001) and miR155 (p = 0.007) were significantly higher in lung cancer patients than those in patients with benign disease. As determined by the Spearman-Rho test, high levels of cell-free DNA significantly correlated with elevated circulating caspase activities (p = 0.0001). In lung cancer patients high serum miR10b values associated with lymph node metastasis (p <0.03) and elevated levels of TPA (tissue polypeptide antigen, p = 0.01), whereas high serum miR141 values associated with elevated levels of uPA (urokinase plasminogen activator, p = 0.02). The findings of our pilot study suggest that the assays for circulating DNA, microRNAs and caspase activities in blood might become novel minimally invasive diagnostic tools for detection and risk assessment of lung cancer, provided that their clinical utility can be confirmed in larger prospective trials.
KW - Adult
KW - Humans
KW - Aged
KW - Middle Aged
KW - Cell Line, Tumor
KW - Gene Expression Regulation, Neoplastic
KW - Gene Expression Profiling
KW - Caspases/blood
KW - DNA, Neoplasm/blood
KW - Lung Neoplasms/blood/diagnosis/enzymology/genetics
KW - Mass Screening
KW - MicroRNAs/genetics/metabolism
KW - Nucleic Acids/blood
KW - Adult
KW - Humans
KW - Aged
KW - Middle Aged
KW - Cell Line, Tumor
KW - Gene Expression Regulation, Neoplastic
KW - Gene Expression Profiling
KW - Caspases/blood
KW - DNA, Neoplasm/blood
KW - Lung Neoplasms/blood/diagnosis/enzymology/genetics
KW - Mass Screening
KW - MicroRNAs/genetics/metabolism
KW - Nucleic Acids/blood
U2 - 10.1016/j.molonc.2011.02.002
DO - 10.1016/j.molonc.2011.02.002
M3 - SCORING: Journal article
VL - 5
SP - 281
EP - 291
JO - MOL ONCOL
JF - MOL ONCOL
SN - 1574-7891
IS - 3
M1 - 3
ER -
