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Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells.

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Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells. / Brundert, May; Heeren, Jörg; Merkel, Martin; Carambia, Antonella; Herkel, Johannes; Groitl, Peter; Dobner, Thomas; Ramakrishnan, Rajasekhar; Moore, Kathryn J; Rinninger, Franz.

in: J LIPID RES, Jahrgang 52, Nr. 4, 4, 2011, S. 745-758.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Brundert, M, Heeren, J, Merkel, M, Carambia, A, Herkel, J, Groitl, P, Dobner, T, Ramakrishnan, R, Moore, KJ & Rinninger, F 2011, 'Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells.', J LIPID RES, Jg. 52, Nr. 4, 4, S. 745-758. <http://www.ncbi.nlm.nih.gov/pubmed/21217164?dopt=Citation>

APA

Brundert, M., Heeren, J., Merkel, M., Carambia, A., Herkel, J., Groitl, P., Dobner, T., Ramakrishnan, R., Moore, K. J., & Rinninger, F. (2011). Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells. J LIPID RES, 52(4), 745-758. [4]. http://www.ncbi.nlm.nih.gov/pubmed/21217164?dopt=Citation

Vancouver

Brundert M, Heeren J, Merkel M, Carambia A, Herkel J, Groitl P et al. Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells. J LIPID RES. 2011;52(4):745-758. 4.

Bibtex

@article{2b45ca9a73b54701839e4b033e049ba7,
title = "Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells.",
abstract = "The mechanisms of HDL-mediated cholesterol transport from peripheral tissues to the liver are incompletely defined. Here the function of scavenger receptor cluster of differentiation 36 (CD36) for HDL uptake by the liver was investigated. CD36 knockout (KO) mice, which were the model, have a 37% increase (P = 0.008) of plasma HDL cholesterol compared with wild-type (WT) littermates. To explore the mechanism of this increase, HDL metabolism was investigated with HDL radiolabeled in the apolipoprotein (¹²?I) and cholesteryl ester (CE, [³H]) moiety. Liver uptake of [³H] and ¹²?I from HDL decreased in CD36 KO mice and the difference, i. e. hepatic selective CE uptake ([³H]¹²?I), declined (-33%, P = 0.0003) in CD36 KO compared with WT mice. Hepatic HDL holo-particle uptake (¹²?I) decreased (-29%, P = 0.0038) in CD36 KO mice. In vitro, uptake of ¹²?I-/[³H]HDL by primary liver cells from WT or CD36 KO mice revealed a diminished HDL uptake in CD36-deficient hepatocytes. Adenovirus-mediated expression of CD36 in cells induced an increase in selective CE uptake from HDL and a stimulation of holo-particle internalization. In conclusion, CD36 plays a role in HDL uptake in mice and by cultured cells. A physiologic function of CD36 in HDL metabolism in vivo is suggested.",
keywords = "Animals, Cells, Cultured, Mice, Mice, Knockout, Immunoblotting, Cell Line, Antigens, CD36/genetics/*metabolism, Biological Transport/genetics/physiology, Cholesterol Esters/metabolism, Hepatocytes/metabolism, Lipoproteins, HDL/*metabolism, Animals, Cells, Cultured, Mice, Mice, Knockout, Immunoblotting, Cell Line, Antigens, CD36/genetics/*metabolism, Biological Transport/genetics/physiology, Cholesterol Esters/metabolism, Hepatocytes/metabolism, Lipoproteins, HDL/*metabolism",
author = "May Brundert and J{\"o}rg Heeren and Martin Merkel and Antonella Carambia and Johannes Herkel and Peter Groitl and Thomas Dobner and Rajasekhar Ramakrishnan and Moore, {Kathryn J} and Franz Rinninger",
year = "2011",
language = "English",
volume = "52",
pages = "745--758",
journal = "J LIPID RES",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells.

AU - Brundert, May

AU - Heeren, Jörg

AU - Merkel, Martin

AU - Carambia, Antonella

AU - Herkel, Johannes

AU - Groitl, Peter

AU - Dobner, Thomas

AU - Ramakrishnan, Rajasekhar

AU - Moore, Kathryn J

AU - Rinninger, Franz

PY - 2011

Y1 - 2011

N2 - The mechanisms of HDL-mediated cholesterol transport from peripheral tissues to the liver are incompletely defined. Here the function of scavenger receptor cluster of differentiation 36 (CD36) for HDL uptake by the liver was investigated. CD36 knockout (KO) mice, which were the model, have a 37% increase (P = 0.008) of plasma HDL cholesterol compared with wild-type (WT) littermates. To explore the mechanism of this increase, HDL metabolism was investigated with HDL radiolabeled in the apolipoprotein (¹²?I) and cholesteryl ester (CE, [³H]) moiety. Liver uptake of [³H] and ¹²?I from HDL decreased in CD36 KO mice and the difference, i. e. hepatic selective CE uptake ([³H]¹²?I), declined (-33%, P = 0.0003) in CD36 KO compared with WT mice. Hepatic HDL holo-particle uptake (¹²?I) decreased (-29%, P = 0.0038) in CD36 KO mice. In vitro, uptake of ¹²?I-/[³H]HDL by primary liver cells from WT or CD36 KO mice revealed a diminished HDL uptake in CD36-deficient hepatocytes. Adenovirus-mediated expression of CD36 in cells induced an increase in selective CE uptake from HDL and a stimulation of holo-particle internalization. In conclusion, CD36 plays a role in HDL uptake in mice and by cultured cells. A physiologic function of CD36 in HDL metabolism in vivo is suggested.

AB - The mechanisms of HDL-mediated cholesterol transport from peripheral tissues to the liver are incompletely defined. Here the function of scavenger receptor cluster of differentiation 36 (CD36) for HDL uptake by the liver was investigated. CD36 knockout (KO) mice, which were the model, have a 37% increase (P = 0.008) of plasma HDL cholesterol compared with wild-type (WT) littermates. To explore the mechanism of this increase, HDL metabolism was investigated with HDL radiolabeled in the apolipoprotein (¹²?I) and cholesteryl ester (CE, [³H]) moiety. Liver uptake of [³H] and ¹²?I from HDL decreased in CD36 KO mice and the difference, i. e. hepatic selective CE uptake ([³H]¹²?I), declined (-33%, P = 0.0003) in CD36 KO compared with WT mice. Hepatic HDL holo-particle uptake (¹²?I) decreased (-29%, P = 0.0038) in CD36 KO mice. In vitro, uptake of ¹²?I-/[³H]HDL by primary liver cells from WT or CD36 KO mice revealed a diminished HDL uptake in CD36-deficient hepatocytes. Adenovirus-mediated expression of CD36 in cells induced an increase in selective CE uptake from HDL and a stimulation of holo-particle internalization. In conclusion, CD36 plays a role in HDL uptake in mice and by cultured cells. A physiologic function of CD36 in HDL metabolism in vivo is suggested.

KW - Animals

KW - Cells, Cultured

KW - Mice

KW - Mice, Knockout

KW - Immunoblotting

KW - Cell Line

KW - Antigens, CD36/genetics/metabolism

KW - Biological Transport/genetics/physiology

KW - Cholesterol Esters/metabolism

KW - Hepatocytes/metabolism

KW - Lipoproteins, HDL/metabolism

KW - Animals

KW - Cells, Cultured

KW - Mice

KW - Mice, Knockout

KW - Immunoblotting

KW - Cell Line

KW - Antigens, CD36/genetics/metabolism

KW - Biological Transport/genetics/physiology

KW - Cholesterol Esters/metabolism

KW - Hepatocytes/metabolism

KW - Lipoproteins, HDL/metabolism

M3 - SCORING: Journal article

VL - 52

SP - 745

EP - 758

JO - J LIPID RES

JF - J LIPID RES

SN - 0022-2275

IS - 4

M1 - 4

ER -