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Sample displacement batch chromatography of proteins

Standard

Sample displacement batch chromatography of proteins. / Kotasinska, Marta; Richter, Verena; Kwiatkowski, Marcel; Schlüter, Hartmut.

in: Methods Mol Biol, Jahrgang 1129, 01.01.2014, S. 325-38.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kotasinska, M, Richter, V, Kwiatkowski, M & Schlüter, H 2014, 'Sample displacement batch chromatography of proteins', Methods Mol Biol, Jg. 1129, S. 325-38. https://doi.org/10.1007/978-1-62703-977-2_24

APA

Kotasinska, M., Richter, V., Kwiatkowski, M., & Schlüter, H. (2014). Sample displacement batch chromatography of proteins. Methods Mol Biol, 1129, 325-38. https://doi.org/10.1007/978-1-62703-977-2_24

Vancouver

Kotasinska M, Richter V, Kwiatkowski M, Schlüter H. Sample displacement batch chromatography of proteins. Methods Mol Biol. 2014 Jan 1;1129:325-38. https://doi.org/10.1007/978-1-62703-977-2_24

Bibtex

@article{000673f83c9b443087df981211731396,
title = "Sample displacement batch chromatography of proteins",
abstract = "In downstream processing large scale chromatography plays an important role. For its development screening experiments followed by pilot plant chromatography are mandatory steps. Here we describe fast, simple, and inexpensive methods for establishing a preparative chromatography for the separation of complex protein mixtures, based on sample displacement batch chromatography. The methods are demonstrated by anion-exchange chromatography of a human plasma protein fraction (Cohn IV-4), including the screening step and scaling up of the chromatography by a factor of 100. The results of the screening experiments and the preparative chromatography are monitored by SDS-PAGE electrophoresis. In summary we provide a protocol which should be easily adaptable for the chromatographic large scale purification of other proteins, in the laboratory as well as in industry for commercial manufacturing. For the latter these protocols cover the initial piloting steps for establishing a sample batch chromatography based on packed columns rather than batch chromatography.",
author = "Marta Kotasinska and Verena Richter and Marcel Kwiatkowski and Hartmut Schl{\"u}ter",
year = "2014",
month = jan,
day = "1",
doi = "10.1007/978-1-62703-977-2_24",
language = "English",
volume = "1129",
pages = "325--38",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Sample displacement batch chromatography of proteins

AU - Kotasinska, Marta

AU - Richter, Verena

AU - Kwiatkowski, Marcel

AU - Schlüter, Hartmut

PY - 2014/1/1

Y1 - 2014/1/1

N2 - In downstream processing large scale chromatography plays an important role. For its development screening experiments followed by pilot plant chromatography are mandatory steps. Here we describe fast, simple, and inexpensive methods for establishing a preparative chromatography for the separation of complex protein mixtures, based on sample displacement batch chromatography. The methods are demonstrated by anion-exchange chromatography of a human plasma protein fraction (Cohn IV-4), including the screening step and scaling up of the chromatography by a factor of 100. The results of the screening experiments and the preparative chromatography are monitored by SDS-PAGE electrophoresis. In summary we provide a protocol which should be easily adaptable for the chromatographic large scale purification of other proteins, in the laboratory as well as in industry for commercial manufacturing. For the latter these protocols cover the initial piloting steps for establishing a sample batch chromatography based on packed columns rather than batch chromatography.

AB - In downstream processing large scale chromatography plays an important role. For its development screening experiments followed by pilot plant chromatography are mandatory steps. Here we describe fast, simple, and inexpensive methods for establishing a preparative chromatography for the separation of complex protein mixtures, based on sample displacement batch chromatography. The methods are demonstrated by anion-exchange chromatography of a human plasma protein fraction (Cohn IV-4), including the screening step and scaling up of the chromatography by a factor of 100. The results of the screening experiments and the preparative chromatography are monitored by SDS-PAGE electrophoresis. In summary we provide a protocol which should be easily adaptable for the chromatographic large scale purification of other proteins, in the laboratory as well as in industry for commercial manufacturing. For the latter these protocols cover the initial piloting steps for establishing a sample batch chromatography based on packed columns rather than batch chromatography.

U2 - 10.1007/978-1-62703-977-2_24

DO - 10.1007/978-1-62703-977-2_24

M3 - SCORING: Journal article

C2 - 24648085

VL - 1129

SP - 325

EP - 338

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -