Salmonella phage ST64B encodes a member of the SseK/NleB effector family.
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Salmonella phage ST64B encodes a member of the SseK/NleB effector family. / Brown, Nat F; Coombes, Brian K; Bishop, Jenna L; Wickham, Mark E; Lowden, Michael J; Gal-Mor, Ohad; Goode, David L; Boyle, Colleen Erin; Sanderson, Kristy L; Finlay, B Brett.
in: PLOS ONE, Jahrgang 6, Nr. 3, 3, 2011, S. 17824.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Salmonella phage ST64B encodes a member of the SseK/NleB effector family.
AU - Brown, Nat F
AU - Coombes, Brian K
AU - Bishop, Jenna L
AU - Wickham, Mark E
AU - Lowden, Michael J
AU - Gal-Mor, Ohad
AU - Goode, David L
AU - Boyle, Colleen Erin
AU - Sanderson, Kristy L
AU - Finlay, B Brett
PY - 2011
Y1 - 2011
N2 - Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease associated with a significant mortality rate. Type III secreted effectors are major contributors to the pathogenesis of Salmonella infections. Genes encoding effectors are acquired via horizontal gene transfer, and a subset are encoded within active phage lysogens. Because the acquisition of effectors is in flux, the complement of effectors possessed by various Salmonella strains frequently differs. By comparing the genome sequences of S. enterica serovar Typhimurium strain SL1344 with LT2, we identified a gene with significant similarity to SseK/NleB type III secreted effector proteins within a phage ST64B lysogen that is absent from LT2. We have named this gene sseK3. SseK3 was co-regulated with the SPI-2 type III secretion system in vitro and inside host cells, and was also injected into infected host cells. While no role for SseK3 in virulence could be identified, a role for the other family members in murine typhoid was found. SseK3 and other phage-encoded effectors were found to have a significant but sparse distribution in the available Salmonella genome sequences, indicating the potential for more uncharacterised effectors to be present in less studied serovars. These phage-encoded effectors may be principle subjects of contemporary selective processes shaping Salmonella-host interactions.
AB - Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease associated with a significant mortality rate. Type III secreted effectors are major contributors to the pathogenesis of Salmonella infections. Genes encoding effectors are acquired via horizontal gene transfer, and a subset are encoded within active phage lysogens. Because the acquisition of effectors is in flux, the complement of effectors possessed by various Salmonella strains frequently differs. By comparing the genome sequences of S. enterica serovar Typhimurium strain SL1344 with LT2, we identified a gene with significant similarity to SseK/NleB type III secreted effector proteins within a phage ST64B lysogen that is absent from LT2. We have named this gene sseK3. SseK3 was co-regulated with the SPI-2 type III secretion system in vitro and inside host cells, and was also injected into infected host cells. While no role for SseK3 in virulence could be identified, a role for the other family members in murine typhoid was found. SseK3 and other phage-encoded effectors were found to have a significant but sparse distribution in the available Salmonella genome sequences, indicating the potential for more uncharacterised effectors to be present in less studied serovars. These phage-encoded effectors may be principle subjects of contemporary selective processes shaping Salmonella-host interactions.
KW - Animals
KW - Mice
KW - Protein Transport
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Base Sequence
KW - Sequence Homology, Amino Acid
KW - Flow Cytometry
KW - Cell Line
KW - DNA Primers
KW - Bacterial Proteins/chemistry/genetics/metabolism
KW - Genes, Viral
KW - Genome, Viral
KW - Salmonella/pathogenicity
KW - Salmonella Phages/genetics
KW - Virulence
KW - Animals
KW - Mice
KW - Protein Transport
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Base Sequence
KW - Sequence Homology, Amino Acid
KW - Flow Cytometry
KW - Cell Line
KW - DNA Primers
KW - Bacterial Proteins/chemistry/genetics/metabolism
KW - Genes, Viral
KW - Genome, Viral
KW - Salmonella/pathogenicity
KW - Salmonella Phages/genetics
KW - Virulence
M3 - SCORING: Journal article
VL - 6
SP - 17824
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 3
M1 - 3
ER -
