Role for Reelin in neurotransmitter release.

Sabine Hellwig; Iris Hack; Janina Kowalski; Bianka Brunne; Joel Jarowyj; Andreas Unger; Hans H Bock; Dirk Junghans; Michael Frotscher

Role for Reelin in neurotransmitter release.

Standard

Role for Reelin in neurotransmitter release. / Hellwig, Sabine; Hack, Iris; Kowalski, Janina; Brunne, Bianka; Jarowyj, Joel; Unger, Andreas; Bock, Hans H; Junghans, Dirk; Frotscher, Michael.

in: J NEUROSCI, Jahrgang 31, Nr. 7, 7, 2011, S. 2352-2360.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hellwig, S, Hack, I, Kowalski, J, Brunne, B, Jarowyj, J, Unger, A, Bock, HH, Junghans, D & Frotscher, M 2011, 'Role for Reelin in neurotransmitter release.', J NEUROSCI, Jg. 31, Nr. 7, 7, S. 2352-2360. <http://www.ncbi.nlm.nih.gov/pubmed/21325502?dopt=Citation>

APA

Hellwig, S., Hack, I., Kowalski, J., Brunne, B., Jarowyj, J., Unger, A., Bock, H. H., Junghans, D., & Frotscher, M. (2011). Role for Reelin in neurotransmitter release. J NEUROSCI, 31(7), 2352-2360. [7]. http://www.ncbi.nlm.nih.gov/pubmed/21325502?dopt=Citation

Vancouver

Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A et al. Role for Reelin in neurotransmitter release. J NEUROSCI. 2011;31(7):2352-2360. 7.

Bibtex

@article{d1c5a8b0a20f41f1882d30e802e51746,

title = "Role for Reelin in neurotransmitter release.",

abstract = "The extracellular matrix molecule Reelin is known to control neuronal migration during development. Recent evidence suggests that it also plays a role in the maturation of postsynaptic dendrites and spines as well as in synaptic plasticity. Here, we aimed to address the question whether Reelin plays a role in presynaptic structural organization and function. Quantitative electron microscopic analysis of the number of presynaptic boutons in the stratum radiatum of hippocampal region CA1 did not reveal differences between wild-type animals and Reelin-deficient reeler mutant mice. However, additional detailed analysis showed that the number of presynaptic vesicles was significantly increased in CA1 synapses of reeler mutants. To test the hypothesis that vesicle fusion is altered in reeler, we studied proteins known to control transmitter release. SNAP25, a protein of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, was found to be significantly reduced in reeler mutants, whereas other SNARE complex proteins remained unaltered. Addition of recombinant Reelin to organotypic slice cultures of reeler hippocampi substantially rescued not only SNAP25 protein expression levels but also the number of vesicles per bouton area indicating a role for Reelin in presynaptic functions. Next, we analyzed paired-pulse facilitation, a presynaptic mechanism associated with transmitter release, and observed a significant decrease at CA1 synapses of reeler mutants when compared with wild-type animals. Together, these novel findings suggest a role for Reelin in modulating presynaptic release mechanisms.",

keywords = "Animals, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Animals, Newborn, Patch-Clamp Techniques, Mutation/genetics, Enzyme Inhibitors/pharmacology, Antibodies/pharmacology, Cell Line, Transformed, Transfection/methods, Green Fluorescent Proteins/genetics, Action Potentials/drug effects/genetics, Antigens, CD29/metabolism, CA1 Region, Hippocampal/cytology, Cell Adhesion Molecules, Neuronal/deficiency/immunology/pharmacology/*physiology, Clathrin/metabolism, Culture Media, Conditioned/pharmacology, Electron Microscope Tomography/methods, Excitatory Postsynaptic Potentials/drug effects/genetics, Extracellular Matrix Proteins/deficiency/immunology/pharmacology/*physiology, Gene Expression Regulation/drug effects/genetics, LDL-Receptor Related Proteins/genetics, Nerve Tissue Proteins/deficiency/genetics/immunology/metabolism/pharmacology/*physiology, Neurotransmitter Agents/*metabolism, Presynaptic Terminals/*metabolism/ultrastructure, R-SNARE Proteins/metabolism, Receptors, LDL/genetics, Serine Endopeptidases/deficiency/immunology/pharmacology/*physiology, Synapses/drug effects/metabolism/ultrastructure, Synaptic Vesicles/metabolism/ultrastructure, Synaptosomal-Associated Protein 25/metabolism, Animals, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Animals, Newborn, Patch-Clamp Techniques, Mutation/genetics, Enzyme Inhibitors/pharmacology, Antibodies/pharmacology, Cell Line, Transformed, Transfection/methods, Green Fluorescent Proteins/genetics, Action Potentials/drug effects/genetics, Antigens, CD29/metabolism, CA1 Region, Hippocampal/cytology, Cell Adhesion Molecules, Neuronal/deficiency/immunology/pharmacology/*physiology, Clathrin/metabolism, Culture Media, Conditioned/pharmacology, Electron Microscope Tomography/methods, Excitatory Postsynaptic Potentials/drug effects/genetics, Extracellular Matrix Proteins/deficiency/immunology/pharmacology/*physiology, Gene Expression Regulation/drug effects/genetics, LDL-Receptor Related Proteins/genetics, Nerve Tissue Proteins/deficiency/genetics/immunology/metabolism/pharmacology/*physiology, Neurotransmitter Agents/*metabolism, Presynaptic Terminals/*metabolism/ultrastructure, R-SNARE Proteins/metabolism, Receptors, LDL/genetics, Serine Endopeptidases/deficiency/immunology/pharmacology/*physiology, Synapses/drug effects/metabolism/ultrastructure, Synaptic Vesicles/metabolism/ultrastructure, Synaptosomal-Associated Protein 25/metabolism",

author = "Sabine Hellwig and Iris Hack and Janina Kowalski and Bianka Brunne and Joel Jarowyj and Andreas Unger and Bock, {Hans H} and Dirk Junghans and Michael Frotscher",

year = "2011",

language = "English",

volume = "31",

pages = "2352--2360",

journal = "J NEUROSCI",

issn = "0270-6474",

publisher = "Society for Neuroscience",

number = "7",

}

RIS

TY - JOUR

T1 - Role for Reelin in neurotransmitter release.

AU - Hellwig, Sabine

AU - Hack, Iris

AU - Kowalski, Janina

AU - Brunne, Bianka

AU - Jarowyj, Joel

AU - Unger, Andreas

AU - Bock, Hans H

AU - Junghans, Dirk

AU - Frotscher, Michael

PY - 2011

Y1 - 2011

N2 - The extracellular matrix molecule Reelin is known to control neuronal migration during development. Recent evidence suggests that it also plays a role in the maturation of postsynaptic dendrites and spines as well as in synaptic plasticity. Here, we aimed to address the question whether Reelin plays a role in presynaptic structural organization and function. Quantitative electron microscopic analysis of the number of presynaptic boutons in the stratum radiatum of hippocampal region CA1 did not reveal differences between wild-type animals and Reelin-deficient reeler mutant mice. However, additional detailed analysis showed that the number of presynaptic vesicles was significantly increased in CA1 synapses of reeler mutants. To test the hypothesis that vesicle fusion is altered in reeler, we studied proteins known to control transmitter release. SNAP25, a protein of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, was found to be significantly reduced in reeler mutants, whereas other SNARE complex proteins remained unaltered. Addition of recombinant Reelin to organotypic slice cultures of reeler hippocampi substantially rescued not only SNAP25 protein expression levels but also the number of vesicles per bouton area indicating a role for Reelin in presynaptic functions. Next, we analyzed paired-pulse facilitation, a presynaptic mechanism associated with transmitter release, and observed a significant decrease at CA1 synapses of reeler mutants when compared with wild-type animals. Together, these novel findings suggest a role for Reelin in modulating presynaptic release mechanisms.

AB - The extracellular matrix molecule Reelin is known to control neuronal migration during development. Recent evidence suggests that it also plays a role in the maturation of postsynaptic dendrites and spines as well as in synaptic plasticity. Here, we aimed to address the question whether Reelin plays a role in presynaptic structural organization and function. Quantitative electron microscopic analysis of the number of presynaptic boutons in the stratum radiatum of hippocampal region CA1 did not reveal differences between wild-type animals and Reelin-deficient reeler mutant mice. However, additional detailed analysis showed that the number of presynaptic vesicles was significantly increased in CA1 synapses of reeler mutants. To test the hypothesis that vesicle fusion is altered in reeler, we studied proteins known to control transmitter release. SNAP25, a protein of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, was found to be significantly reduced in reeler mutants, whereas other SNARE complex proteins remained unaltered. Addition of recombinant Reelin to organotypic slice cultures of reeler hippocampi substantially rescued not only SNAP25 protein expression levels but also the number of vesicles per bouton area indicating a role for Reelin in presynaptic functions. Next, we analyzed paired-pulse facilitation, a presynaptic mechanism associated with transmitter release, and observed a significant decrease at CA1 synapses of reeler mutants when compared with wild-type animals. Together, these novel findings suggest a role for Reelin in modulating presynaptic release mechanisms.

KW - Animals

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Animals, Newborn

KW - Patch-Clamp Techniques

KW - Mutation/genetics

KW - Enzyme Inhibitors/pharmacology

KW - Antibodies/pharmacology

KW - Cell Line, Transformed

KW - Transfection/methods

KW - Green Fluorescent Proteins/genetics

KW - Action Potentials/drug effects/genetics

KW - Antigens, CD29/metabolism

KW - CA1 Region, Hippocampal/cytology

KW - Cell Adhesion Molecules, Neuronal/deficiency/immunology/pharmacology/physiology

KW - Clathrin/metabolism

KW - Culture Media, Conditioned/pharmacology

KW - Electron Microscope Tomography/methods

KW - Excitatory Postsynaptic Potentials/drug effects/genetics

KW - Extracellular Matrix Proteins/deficiency/immunology/pharmacology/physiology

KW - Gene Expression Regulation/drug effects/genetics

KW - LDL-Receptor Related Proteins/genetics

KW - Nerve Tissue Proteins/deficiency/genetics/immunology/metabolism/pharmacology/physiology

KW - Neurotransmitter Agents/metabolism

KW - Presynaptic Terminals/metabolism/ultrastructure

KW - R-SNARE Proteins/metabolism

KW - Receptors, LDL/genetics

KW - Serine Endopeptidases/deficiency/immunology/pharmacology/physiology

KW - Synapses/drug effects/metabolism/ultrastructure

KW - Synaptic Vesicles/metabolism/ultrastructure

KW - Synaptosomal-Associated Protein 25/metabolism