RGD-peptides for tissue engineering of articular cartilage.
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RGD-peptides for tissue engineering of articular cartilage. / Jeschke, Brigitte; Meyer, Jörg; Jonczyk, Alfred; Kessler, Horst; Adamietz, Peter; Meenen, Norbert; Kantlehner, Martin; Goepfert, Christiane; Nies, Berthold.
in: BIOMATERIALS, Jahrgang 23, Nr. 16, 16, 2002, S. 3455-3463.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - RGD-peptides for tissue engineering of articular cartilage.
AU - Jeschke, Brigitte
AU - Meyer, Jörg
AU - Jonczyk, Alfred
AU - Kessler, Horst
AU - Adamietz, Peter
AU - Meenen, Norbert
AU - Kantlehner, Martin
AU - Goepfert, Christiane
AU - Nies, Berthold
PY - 2002
Y1 - 2002
N2 - One keypoint in the development of a biohybrid implant for articular cartilage defects is the specific binding of cartilage cells to a supporting structure. Mimicking the physiological adhesion process of chondrocytes to the extracellular matrix is expected to improve cell adhesion of in vitro cultured chondrocytes. Our approach involves coating of synthetic scaffolds with tailor-made, cyclic RGD-peptides, which bind to specific integrin receptors on the cell surface. In this study we investigated the expression pattern of integrins on the cell surface of chondrocytes and their capability to specifically bind to RGD-peptide coated materials in the course of monolayer cultivation. Human chondrocytes expressed integrins during a cultivation period of 20 weeks. Receptors proved to be functionally active as human and pig chondrocytes attached to RGD-coated surfaces. A competition assay with soluble RGD-peptide revealed binding specificity to the RGD-entity. Chondrocyte morphology changed with increasing amounts of cyclic RGD-peptides on the surface.
AB - One keypoint in the development of a biohybrid implant for articular cartilage defects is the specific binding of cartilage cells to a supporting structure. Mimicking the physiological adhesion process of chondrocytes to the extracellular matrix is expected to improve cell adhesion of in vitro cultured chondrocytes. Our approach involves coating of synthetic scaffolds with tailor-made, cyclic RGD-peptides, which bind to specific integrin receptors on the cell surface. In this study we investigated the expression pattern of integrins on the cell surface of chondrocytes and their capability to specifically bind to RGD-peptide coated materials in the course of monolayer cultivation. Human chondrocytes expressed integrins during a cultivation period of 20 weeks. Receptors proved to be functionally active as human and pig chondrocytes attached to RGD-coated surfaces. A competition assay with soluble RGD-peptide revealed binding specificity to the RGD-entity. Chondrocyte morphology changed with increasing amounts of cyclic RGD-peptides on the surface.
KW - Humans
KW - Cells, Cultured
KW - Amino Acid Sequence
KW - Tissue Engineering/methods
KW - Cartilage, Articular/cytology
KW - Cell Adhesion/drug effects/physiology
KW - Chondrocytes/cytology/drug effects/physiology
KW - Oligopeptides/chemical synthesis/chemistry
KW - Humans
KW - Cells, Cultured
KW - Amino Acid Sequence
KW - Tissue Engineering/methods
KW - Cartilage, Articular/cytology
KW - Cell Adhesion/drug effects/physiology
KW - Chondrocytes/cytology/drug effects/physiology
KW - Oligopeptides/chemical synthesis/chemistry
M3 - SCORING: Journal article
VL - 23
SP - 3455
EP - 3463
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 16
M1 - 16
ER -
