Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces.
Standard
Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces. / Briem, D; Strametz, S; Schröder, K; Meenen, Norbert; Lehmann, Wolfgang; Linhart, W; Ohl, A; Rueger, J M.
in: J MATER SCI-MATER M, Jahrgang 16, Nr. 7, 7, 2005, S. 671-677.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces.
AU - Briem, D
AU - Strametz, S
AU - Schröder, K
AU - Meenen, Norbert
AU - Lehmann, Wolfgang
AU - Linhart, W
AU - Ohl, A
AU - Rueger, J M
PY - 2005
Y1 - 2005
N2 - Polyetheretherketone (PEEK) is a synthetic polymer with suitable biomechanical and stable chemical properties, which make it attractive for use as an endoprothetic material and for ligamentous replacement. However, chemical surface inertness does not account for a good interfacial biocompatibility, and PEEK requires a surface modification prior to its application in vivo. In the course of this experimental study we analyzed the influence of plasma treatment of PEEK surfaces on the cell proliferation and differentiation of primary fibroblasts and osteoblasts. Further we examined the possibility of inducing microstructured cell growth on a surface with plasma-induced chemical micropatterning. We were able to demonstrate that the surface treatment of PEEK with a low-temperature plasma has significant effects on the proliferation of fibroblasts. Depending on the surface treatment, the proliferation rate can either be stimulated or suppressed. The behavior of the osteoblasts was examined by evaluating differentiation parameters. By detection of alkaline phosphatase, collagen I, and mineralized extracellular matrix as parameters for osteoblastic differentiation, the examined materials showed results comparable to commercially available polymer cell culture materials such as tissue culture polystyrene (TCPS). Further microstructured cell growth was produced successfully on micropatterned PEEK foils, which could be a future tool for bioartificial systems applying the methods of tissue engineering. These results show that chemically inert materials such as PEEK may be modified specifically through the methods of plasma technology in order to improve biocompatibility.
AB - Polyetheretherketone (PEEK) is a synthetic polymer with suitable biomechanical and stable chemical properties, which make it attractive for use as an endoprothetic material and for ligamentous replacement. However, chemical surface inertness does not account for a good interfacial biocompatibility, and PEEK requires a surface modification prior to its application in vivo. In the course of this experimental study we analyzed the influence of plasma treatment of PEEK surfaces on the cell proliferation and differentiation of primary fibroblasts and osteoblasts. Further we examined the possibility of inducing microstructured cell growth on a surface with plasma-induced chemical micropatterning. We were able to demonstrate that the surface treatment of PEEK with a low-temperature plasma has significant effects on the proliferation of fibroblasts. Depending on the surface treatment, the proliferation rate can either be stimulated or suppressed. The behavior of the osteoblasts was examined by evaluating differentiation parameters. By detection of alkaline phosphatase, collagen I, and mineralized extracellular matrix as parameters for osteoblastic differentiation, the examined materials showed results comparable to commercially available polymer cell culture materials such as tissue culture polystyrene (TCPS). Further microstructured cell growth was produced successfully on micropatterned PEEK foils, which could be a future tool for bioartificial systems applying the methods of tissue engineering. These results show that chemically inert materials such as PEEK may be modified specifically through the methods of plasma technology in order to improve biocompatibility.
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Surface Properties
KW - Cell Proliferation
KW - Materials Testing
KW - Hot Temperature
KW - Cell Culture Techniques/methods
KW - Tissue Engineering/methods
KW - Cell Survival
KW - Biocompatible Materials/analysis/chemistry
KW - Fibroblasts/cytology/physiology
KW - Gases/chemistry
KW - Ketones/analysis/chemistry
KW - Osteoblasts/chemistry/cytology
KW - Polyethylene Glycols/analysis/chemistry
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Surface Properties
KW - Cell Proliferation
KW - Materials Testing
KW - Hot Temperature
KW - Cell Culture Techniques/methods
KW - Tissue Engineering/methods
KW - Cell Survival
KW - Biocompatible Materials/analysis/chemistry
KW - Fibroblasts/cytology/physiology
KW - Gases/chemistry
KW - Ketones/analysis/chemistry
KW - Osteoblasts/chemistry/cytology
KW - Polyethylene Glycols/analysis/chemistry
M3 - SCORING: Journal article
VL - 16
SP - 671
EP - 677
JO - J MATER SCI-MATER M
JF - J MATER SCI-MATER M
SN - 0957-4530
IS - 7
M1 - 7
ER -
