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Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion

Standard

Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion. / Arakawa, Hiroshi; Hauschild, Jessica; Buerstedde, Jean-Marie.

in: SCIENCE, Jahrgang 295, Nr. 5558, 15.02.2002, S. 1301-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Arakawa, H, Hauschild, J & Buerstedde, J-M 2002, 'Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion', SCIENCE, Jg. 295, Nr. 5558, S. 1301-6. https://doi.org/10.1126/science.1067308

APA

Arakawa, H., Hauschild, J., & Buerstedde, J-M. (2002). Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion. SCIENCE, 295(5558), 1301-6. https://doi.org/10.1126/science.1067308

Vancouver

Arakawa H, Hauschild J, Buerstedde J-M. Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion. SCIENCE. 2002 Feb 15;295(5558):1301-6. https://doi.org/10.1126/science.1067308

Bibtex

@article{c5f31189bfaa438490d1b7b0f825cee2,
title = "Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion",
abstract = "Three phenotypically distinct processes-somatic hypermutation, gene conversion, and switch recombination-remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of the AID complementary DNA. This demonstrates that the AID master gene controls all B cell-specific modifications of vertebrate Ig genes.",
keywords = "Alleles, Amino Acid Sequence, Animals, B-Lymphocytes, Cell Line, Chickens, Cloning, Molecular, Cytidine Deaminase, DNA, Complementary, Expressed Sequence Tags, Gene Conversion, Gene Expression, Gene Targeting, Genes, Immunoglobulin, Humans, Immunoglobulin Class Switching, Immunoglobulin Light Chains, Immunoglobulin M, Molecular Sequence Data, Mutation, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Somatic Hypermutation, Immunoglobulin, Transfection",
author = "Hiroshi Arakawa and Jessica Hauschild and Jean-Marie Buerstedde",
year = "2002",
month = feb,
day = "15",
doi = "10.1126/science.1067308",
language = "English",
volume = "295",
pages = "1301--6",
journal = "SCIENCE",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5558",

}

RIS

TY - JOUR

T1 - Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion

AU - Arakawa, Hiroshi

AU - Hauschild, Jessica

AU - Buerstedde, Jean-Marie

PY - 2002/2/15

Y1 - 2002/2/15

N2 - Three phenotypically distinct processes-somatic hypermutation, gene conversion, and switch recombination-remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of the AID complementary DNA. This demonstrates that the AID master gene controls all B cell-specific modifications of vertebrate Ig genes.

AB - Three phenotypically distinct processes-somatic hypermutation, gene conversion, and switch recombination-remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of the AID complementary DNA. This demonstrates that the AID master gene controls all B cell-specific modifications of vertebrate Ig genes.

KW - Alleles

KW - Amino Acid Sequence

KW - Animals

KW - B-Lymphocytes

KW - Cell Line

KW - Chickens

KW - Cloning, Molecular

KW - Cytidine Deaminase

KW - DNA, Complementary

KW - Expressed Sequence Tags

KW - Gene Conversion

KW - Gene Expression

KW - Gene Targeting

KW - Genes, Immunoglobulin

KW - Humans

KW - Immunoglobulin Class Switching

KW - Immunoglobulin Light Chains

KW - Immunoglobulin M

KW - Molecular Sequence Data

KW - Mutation

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Alignment

KW - Somatic Hypermutation, Immunoglobulin

KW - Transfection

U2 - 10.1126/science.1067308

DO - 10.1126/science.1067308

M3 - SCORING: Journal article

C2 - 11847344

VL - 295

SP - 1301

EP - 1306

JO - SCIENCE

JF - SCIENCE

SN - 0036-8075

IS - 5558

ER -